Set of enzymatic bioassays for assessment of soil pollution

Cover Page

Abstract


A concept of the comprehensive assessment of soil contamination is proposed. In this concept, the conclusion regarding the presence of toxic substances in a sample, which is being analyzed, is connected with the inhibition of enzymatic reactions that are responsible for various functions of a live organism, such as luminescence, respiration, etc., these functions are taken as test functions in classical bioassays, which are using live objects (luminous bacteria, daphnia, algae, etc.). The regularities of the impact of different classes of toxicants on the activity of individual enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects - markers of pollution. The set of enzymatic bioassays consists of three enzyme systems with maximum sensitivity to different classes of toxicants: butyrylcholinesterase, NAD(P)H:FMN‑oxidoreductase + luciferase and lactate dehydrogenase + NAD(P)H:FMN‑oxidoreductase + luciferase. The possibility of using enzymes instead of living organisms in the bioassay of natural complex systems is shown.


About the authors

Е. М. Kolosova

Siberian Federal University

Email: OSutormin@sfu-kras.ru

Russian Federation, 79, Svobodny avenue, Krasnoyarsk, 660041

О. S. Sutormin

Siberian Federal University

Author for correspondence.
Email: OSutormin@sfu-kras.ru

Russian Federation, 79, Svobodny avenue, Krasnoyarsk, 660041

Е. N. Esimbekova

Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences; Siberian Federal University

Email: OSutormin@sfu-kras.ru

Russian Federation, 50, Akademgorodok, Krasnoyarsk, 660036; 79, Svobodny avenue, Krasnoyarsk, 660041

V. I. Lonshakova-Mukina

Siberian Federal University

Email: OSutormin@sfu-kras.ru

Russian Federation, 79, Svobodny avenue, Krasnoyarsk, 660041

V. А. Kratasyuk

Siberian Federal University; Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences

Email: OSutormin@sfu-kras.ru

Russian Federation, 79, Svobodny avenue, Krasnoyarsk, 660041; 50, Akademgorodok, Krasnoyarsk, 660036

References

  1. Terekhova V., Gladkova M. // Eurasian Soil Sci. 2013. V. 46. P. 1203-1210.
  2. Есимбекова Е. Н. Биолюминесцентные биотесты: современное состояние и перспективы: монография / Есимбекова Е.Н., Кратасюк В. А., Немцева Е. В., Кудряшева Н. С., Медведева С. Е., Кириллова М. А. Красноярск: Сиб. фед. ун-т, 2018. 256 с.
  3. Esimbekova E., Kratasyuk V., Shimomura O. Application of Enzyme Bioluminescence in Ecology // Biochem. Eng. Biotechnol. 2014. V. 144. P. 67-109.
  4. Сутормин О. С., Колосова Е. М., Немцева Е. В. и др. // Цитология. 2018. Т. 60. № 10. С. 826-829.
  5. Nunes B. // Rev. Environ. Contam. Toxicol. 2011. V. 212. P. 29-55.
  6. Семенов А. М., Соколов М. С. // Агрохимия. 2016. № 1. С. 3-16.
  7. Van Gestel C. A.M. // Zookeys. 2012. V. 176. P. 275-296.
  8. Bierkens J., Klein G., Corbisier P., et al. // Chemosphere. 1998. V. 37. № 14/15. P. 2935-2947.

Statistics

Views

Abstract - 113

PDF (Russian) - 89

PlumX


Copyright (c) 2019 Russian academy of sciences

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies