Resistance of THP-1 leukemia cells infected with cytomegalovirus to anti-tumor antibiotic doxorubicin and restoration the sensitivity by inhibitors of the molecular way PI3K/AKT/mTOR

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Abstract

Results obtained showed that infection with HCMV prevented the death of THP‑1 cells treated with DOX in both active and latent forms of infection. In the presence of mTOR inhibitors (rapamycin and Torin2), the sensitivity of the infected cells to DOX was restored. Rapamycin inhibited the expression of HCMV protein IE1-p72 and increased sensitivity to DOX. Molecular targets for the creation of new drugs for the treatment of leukemia in patients infected with HCMV have been determined.

About the authors

Ya. Yu. Chernoryzh

Gamaleya National Research Center for Epidemiology and Microbiology, the Ministry of Health of the Russian Federation

Email: vitallku@mail.ru
Russian Federation, 18, Gamalei street, Moscow, 123098

N. E. Fedorova

Gamaleya National Research Center for Epidemiology and Microbiology, the Ministry of Health of the Russian Federation

Email: vitallku@mail.ru
Russian Federation, 18, Gamalei street, Moscow, 123098

K. I. Yurlov

Gamaleya National Research Center for Epidemiology and Microbiology, the Ministry of Health of the Russian Federation

Email: vitallku@mail.ru
Russian Federation, 18, Gamalei street, Moscow, 123098

R. A. Simonov

Gamaleya National Research Center for Epidemiology and Microbiology, the Ministry of Health of the Russian Federation

Email: vitallku@mail.ru
Russian Federation, 18, Gamalei street, Moscow, 123098

A. B. Kornev

Institute of Problem of Chemical Physics of the Russian Academy of Science

Email: vitallku@mail.ru
Russian Federation, 1, Acad. Semenov av., Chernogolovka, Moscow region, 142432

D. S. Karpov

Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences

Email: vitallku@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991

N. F. Zakirova

Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences

Email: vitallku@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991

A. V. Ivanov

Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences

Email: vitallku@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991

A. A. Kushch

Gamaleya National Research Center for Epidemiology and Microbiology, the Ministry of Health of the Russian Federation

Author for correspondence.
Email: vitallku@mail.ru
Russian Federation, 18, Gamalei street, Moscow, 123098

A. L. Gintsburg

Gamaleya National Research Center for Epidemiology and Microbiology, the Ministry of Health of the Russian Federation; The First Sechenov Moscow State Medical University under the Ministry of Health of the Russian Federation

Email: vitallku@mail.ru

Academician of the Russian Academy of Sciences

Russian Federation, 18, Gamalei street, Moscow, 123098; 8-2, Trubetskaya street, Moscow, 119992

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