The induction of the synthesis of interferon, CD4 and CD8 t lymphocytes in blood and spleen of mice after oral vaccination with the “early” protein HPV16 E2

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Abstract

Very high contents of the interferon, CD4 and CD8 T lymphocytes were found in blood and spleen cells after the oral vaccination of mice with the “early” protein of high-risk oncogenic papillomavirus HPV16 E2 by using immunoassays analyses: ELISPOT for the detection of the content of interferon, Western-blot hybridization and enzyme immunoassays for analyses of СD4 and CD8 T lymphocytes. Both blood cells and splenocytes showed high content of alive mononuclear cells during ELISA and ELISPOT analyses that had very characteristic features for T lymphocytes. The ratio of CD4/CD8 was very close to 1 after the quanttitive determination by the enzyme immunoassays with appropriate antibodies. It was concluded that the “early” protein HPV16 E2 participated in the activation of the immune system during pathogenesis and neoplasia.

About the authors

R. K. Salyaev

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences; The Irkutsk Scientific Center, Siberian Branch of the Russian Academy of Sciences

Author for correspondence.
Email: salyaev@sifibr.irk.ru

Corresponding Member of Russian Academy of Sciences

Russian Federation, 132, Lermontova street, Irkutsk, 664033; 134, Lermontova street, Irkutsk, 664033

N. I. Rekoslavskaya

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences; The Irkutsk Scientific Center, Siberian Branch of the Russian Academy of Sciences

Email: salyaev@sifibr.irk.ru
Russian Federation, 132, Lermontova street, Irkutsk, 664033; 134, Lermontova street, Irkutsk, 664033

A. S. Stolbikov

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences; The Irkutsk Scientific Center, Siberian Branch of the Russian Academy of Sciences

Email: salyaev@sifibr.irk.ru
Russian Federation, 132, Lermontova street, Irkutsk, 664033; 134, Lermontova street, Irkutsk, 664033

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