Email this article The development of reporter system for the investigation of molecular mechanisms of ecdysone response
- Authors: Mazina M.Y.1, Krasnov A.N.1, Georgiev P.G.1, Vorobyeva N.E.1
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Affiliations:
- Institute of Gene Biology of the Russian Academy of Sciences
- Issue: Vol 485, No 4 (2019)
- Pages: 515-518
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/14267
- DOI: https://doi.org/10.31857/S0869-56524854515-518
- ID: 14267
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Abstract
To study the mechanisms of transcriptional regulation, a convenient experimental approach is to use the artificial chimeric constructs, carrying the regulatory elements of interest. In the present work, we describe the creation and characterization of a novel genetic construct, which makes possible to study the transcriptional regulation of the early-late gene of the ecdysone cascade. Using the data of genome-wide experiments, we have isolated the main regulatory region of the hr4 gene, which was successfully used to create a chimeric reporter construct expressing a fluorescent protein upon the treatment with the ecdysone hormone. This reporter system can be used to study the mechanisms of the ecdysone response, both in cell culture and in tissues, at various stages of the Drosophila development.
Keywords
About the authors
M. Yu. Mazina
Institute of Gene Biology of the Russian Academy of Sciences
Email: nvorobyova@gmail.com
Russian Federation, 34/5, Vavilova street, Moscow, 119334
A. N. Krasnov
Institute of Gene Biology of the Russian Academy of Sciences
Email: nvorobyova@gmail.com
Russian Federation, 34/5, Vavilova street, Moscow, 119334
P. G. Georgiev
Institute of Gene Biology of the Russian Academy of Sciences
Email: nvorobyova@gmail.com
Academician of the Russian Academy of Sciences
Russian Federation, 34/5, Vavilova street, Moscow, 119334N. E. Vorobyeva
Institute of Gene Biology of the Russian Academy of Sciences
Author for correspondence.
Email: nvorobyova@gmail.com
Russian Federation, 34/5, Vavilova street, Moscow, 119334
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