The development of reporter system for the investigation of molecular mechanisms of ecdysone response

Cover Page

Abstract


To study the mechanisms of transcriptional regulation, a convenient experimental approach is to use the artificial chimeric constructs, carrying the regulatory elements of interest. In the present work, we describe the creation and characterization of a novel genetic construct, which makes possible to study the transcriptional regulation of the early-late gene of the ecdysone cascade. Using the data of genome-wide experiments, we have isolated the main regulatory region of the hr4 gene, which was successfully used to create a chimeric reporter construct expressing a fluorescent protein upon the treatment with the ecdysone hormone. This reporter system can be used to study the mechanisms of the ecdysone response, both in cell culture and in tissues, at various stages of the Drosophila development.


About the authors

M. Yu. Mazina

Institute of Gene Biology of the Russian Academy of Sciences

Email: nvorobyova@gmail.com

Russian Federation, 34/5, Vavilova street, Moscow, 119334

A. N. Krasnov

Institute of Gene Biology of the Russian Academy of Sciences

Email: nvorobyova@gmail.com

Russian Federation, 34/5, Vavilova street, Moscow, 119334

P. G. Georgiev

Institute of Gene Biology of the Russian Academy of Sciences

Email: nvorobyova@gmail.com

Russian Federation, 34/5, Vavilova street, Moscow, 119334

Academician of the Russian Academy of Sciences

N. E. Vorobyeva

Institute of Gene Biology of the Russian Academy of Sciences

Author for correspondence.
Email: nvorobyova@gmail.com

Russian Federation, 34/5, Vavilova street, Moscow, 119334

References

  1. Mitchell N., Cranna N., Richardson H., Quinn L. // Development. 2008. V. 135. P. 2707-2716.
  2. Krasnov A. N., Mazina M. Y., Nikolenko J. V., Vorobyeva N. E. // Cell Biosci. 2016. V. 6. P. 15.
  3. Mazina M. Y., Kovalenko E. V., Derevyanko P. K., Nikolenko J. V., Krasnov A. N., Vorobyeva N. E. // Biochim. Biophys. Acta. 2018. V. 1861. P. 178-189.
  4. Antoniewski C., Laval M., Lepesant J.-A. // Insect Biochem. Mol. 1993. V. 23 P. 105-114.
  5. Shlyueva D., Stelzer C., Gerlach D., Yáñez-Cuna J.O., Rath M., Boryń Ł. M., Arnold C. D., Stark A. // Mol. Cell. 2014. V. 54. P. 180-192.
  6. Li X., Zhao X., Fang Y., Jiang X., Duong T., Fan C., Huang C. C., Kain S. R. // J. Biol. Chem. 1998. V. 273. P. 34 970-34 975.

Statistics

Views

Abstract - 199

PDF (Russian) - 121

PlumX


Copyright (c) 2019 Russian academy of sciences

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies