Email this article LH2 complexes (B800-850 AND B800-830) are assembled in the cells of sulfur bacterium Thiorhodospira sibirica, strain Kir 3, without any carotenoids
- Authors: Bolshakov M.A.1, Ashikhmin A.A.1, Makhneva Z.K.1, Moskalenko A.A.1
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Affiliations:
- Institute of Basic Biological Problems of the Russian Academy of Sciences
- Issue: Vol 487, No 6 (2019)
- Pages: 701-705
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/15982
- DOI: https://doi.org/10.31857/S0869-56524876701-705
- ID: 15982
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Abstract
It has been studied the result of assembling the light-harvesting complexes in the cells of purple sulfuric bacterium Thiorhodospira (T.) sibirica, strain Kir‑3, while suppressing biosynthesis of carotenoids by diphenylamine (DPA). LH2 complexes (B800-850 and B800-830) with different carotenoids’ composition were isolated from the cells obtained. Maximal inhibition of carotenoid biosynthesis (~90% of the control) was achieved at the inhibitor concentration of 53,25 mM (9 mg/l). It has been established that changes in qualitative and quantitative composition of carotenoids do not affect the assembling of B800-830 and B800-850 complexes. It is assumed that in the population of DPA-LH2 complexes from T. sibirica, strain Kir‑3, both carotenoidless complexes and the complexes, containing one or two carotenoid molecules, can be assembled. These results support a hypothesis that carotenoids are not required for assembling B800-850 and B800-830 complexes.
About the authors
M. A. Bolshakov
Institute of Basic Biological Problems of the Russian Academy of Sciences
Author for correspondence.
Email: lfbv22@gmail.com
Russian Federation, 2, Institutskaya street, Pushchino, Moscow Region, 142290
A. A. Ashikhmin
Institute of Basic Biological Problems of the Russian Academy of Sciences
Email: AshikhminAA@gmail.com
Russian Federation, 2, Institutskaya street, Pushchino, Moscow Region, 142290
Z. K. Makhneva
Institute of Basic Biological Problems of the Russian Academy of Sciences
Email: lfbv22@gmail.com
Russian Federation, 2, Institutskaya street, Pushchino, Moscow Region, 142290
A. A. Moskalenko
Institute of Basic Biological Problems of the Russian Academy of Sciences
Email: lfbv22@gmail.com
Russian Federation, 2, Institutskaya street, Pushchino, Moscow Region, 142290
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