Cytotoxicity and toxicological characteristics of the new leukocytar polypeptide

The aim of the study is to assess the safety of a low molecular weight leukocyte protein-peptide complex (LPPC) at the stage of preclinical studies. The object of the study was an experimental sample of a new leukocyte protein-peptide complex (LPPK) obtained by ultrasonic treatment of human leukocytes in vitro. LPPK cytotoxicity was assessed on the cell line of pig embryonic kidney epithelial cells (SPEV) and in vitro MTT test on human tumor cell lines HCT 116 (colorectal carcinoma) and MS (melanoma). A proliferation index was used to assess the effect on the growth of cells of the leukocyte protein-peptide complex (LBPC). Experiments on the study of acute toxicity of LPPK were performed on white nonlinear mice, which were injected intraperitoneally. To assess the results used the method of determining the average lethal dose according to V. Prozorovsky. When studying cytotoxicity, it was found that the addition of an experimental sample of the leukocyte protein-peptide complex to the growth medium for culturing the SPEV cell line favorably affects cell growth. The proliferation index increased from the first to the fourth passage by 1.09 times, while in the control sample the increase in PI occurred by 1.03 times. A study of the cytotoxicity of LPPK showed that the sample did not exhibit a cytotoxic effect on tumor cells HCT 116 and MS, since the IC50 concentrations calculated for them in all cases were significantly lower than the reference drug Doxorubicin. The results of the assessment of acute toxicity in experimental animals showed that the test sample with intraperitoneal administration is low toxic according to the classification of GOST 12.1.007.-76 (LD50 3500.0 mg / kg). The article demonstrates the results of determining the toxicological and cytotoxic characteristics of the protein-peptide complex obtained from human leukocytes. It was shown that the studied protein-peptide complex did not exhibit toxic effects on the studied cell lines, as well as laboratory animals (mice).

cytotoxicity, SPEV, leukocyte protein-peptide complex, acute toxicity, mice