Study of iridoid accumulation in the vitex agnus-castus fruits different places of growth
- Authors: Adamov G.V1, Saybel O.L1, Dargaeva T.D1, Logvinenko L.A2, Shevchuk O.M2
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Affiliations:
- Federal State Budgetary Institution «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants»
- Federal State Budgetary Institution «Nikitsky Botanical Garden- National Scientific Center of the Russian Academy of Sciences»
- Issue: Vol 23, No 7 (2020)
- Pages: 3-8
- Section: Articles
- URL: https://journals.eco-vector.com/1560-9596/article/view/112759
- DOI: https://doi.org/10.29296/25877313-2020-07-01
- ID: 112759
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Abstract
Relevance. At present, the search for new types of medicinal plants and the creation on their basis of effective medicines remains one of the relevant areas of domestic pharmaceutical science. A promising research object in the direction of creating a domestic drug for the correction of female reproductive system disorders is the chaste tree (Vitex agnus-castus L.) The aim of this work was to study the accumulation of iridoids in the chaste tree fruits to identify differences in the content of this group of biologically active substances depending on the location of the plant. Material and methods. The object of the study was the dried chaste tree fruits, harvested in Macedonia, Greece, Abkhazia, and the Republic of Crimea. The determination of the amount of iridoids was carried out by spectrophotometry according to the reaction of complexation with the Trim-Hill reagent. The agnuside content was evaluated by HPLC-UV. Results. As a result of the research, a study was made of the accumulation of iridoids and agnuside in the chaste tree fruits, harvested in various places where the plant grows. The data obtained allow us to establish a norm of the content of the amount of iridoids in the chaste tree fruits not less than 1%. Conclusion. The developed and validated methodology for assessing the content of the amount of iridoids can be used as a quantitative determination method for standardizing the chaste tree fruits, and the selected conditions of the HPLC analysis of agnuside are used to confirm the authenticity of this raw material. The data obtained will be used in the development of regulatory documentation for a new type of medicinal plant material «Vitex agnus-castus fruit».
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About the authors
G. V Adamov
Federal State Budgetary Institution «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants»
O. L Saybel
Federal State Budgetary Institution «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants»
Email: olster@mail.ru
T. D Dargaeva
Federal State Budgetary Institution «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants»
L. A Logvinenko
Federal State Budgetary Institution «Nikitsky Botanical Garden- National Scientific Center of the Russian Academy of Sciences»
O. M Shevchuk
Federal State Budgetary Institution «Nikitsky Botanical Garden- National Scientific Center of the Russian Academy of Sciences»
References
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