DEVELOPMENT OF THE QUANTITATIVE ANALYSIS METHOD OF THE FLAVONOIDS IN A SOFT DOSAGE FORM WITH PEONY EXTRACT OF GARDEN VARIETIES BASED ON TIZOL GEL

Varieties of garden peonies are of interest in the field of creating phytopreparations for the treatment of dermatological diseases due to its rich chemical composition. An important component in drug development is the assessment of product guality at all stages using methods that give reliable data. In this regard, the article is devoted to the development of a method for guantifying one of the main groups of biologically active substances, such as flavonoids, in a soft dosage form with peony extract of garden varieties dry in order to apply this method at various stages of guality control of this dosage form. For this purpose method of spectrophotometric determination of sum of flavonoids per rutin in extract of peony of garden varieties dry is modified. Integrated researches on the selection of the optimal solvent, the ratio of the recovery volumes and 2% aluminum chloride solution and the stability time of the flavonoid complex with the chromogenic reagent have been carried out since the analysis of the soft dosage form may be problematic due to the need to extract analysable substances from the ointment base. As a result of carried out studies extragent-alcohol ethyl 70%, ointment suspension 1.0 g, aliguot part of extraction of 2 ml, ratio of aliguot to chromogenic reagent 1:1 is chosen, with the time of sustainable complex of 30 minutes. In these conditions, the content of flavonoids in ointments with peony extract of garden varieties of dry 0,338 ± 0,015% is obtained, which is as close as possible to the content of this group of biologically active substances in the extract.

Peony garden varieties, dry extract, Tizol, flavonoids