Dependence of the efficiency of vitamin D deficiency correction and its consequences in rats from supply with B group vitamins

Relevance. Multiple micronutrient deficiencies, the most freguent of which is a lack of vitamin D, then В vitamins, is typical for the population of Russia. Taking cholecalciferol without prescribing В vitamins, which are necessary for its transformation into the hormonal form, is recommended for correcting vitamin D deficiency The aim of the study is to compare the effectiveness of vitamin D deficiency correction in rats against the background of adeguate and insufficient supply of В vitamins. Material and methods. The combined deficiency of vitamins D and group В in male Wistar rats (n = 33) with body weight (69.5 ± 0.8 g) was caused d by a 5-fold decrease in the amount of vitamins in the vitamin mixture of a semi-synthetic diet for 23 days. During the next 7 days, in order to correct vitamin deficiency, 12 rats (group «-В +D») received a diet supplemented to 100% in vitamin D against the background of continuing deficiency of vitamins of group B, and 12 rats (group «+В +D») - a diet supplemented with all the missing vitamins. Animals of the control group (n = 9) received a full-fledged semi-synthetic diet. The content of calcium and iron in the freeze-dried liver and brain was determined on a Z 5300 atomic absorption spectrophotometer (Hitachi High-Technologies Corporation (HHC), Japan), biochemical parameters of blood and urine were determined on a KoneLab 200І biochemical analyzer (ThermoScientific, Finland). Results. Against the background of a deficiency of В vitamins, the addition of cholecalciferol to rats deficient in В and D vitamins did not fully restore the indicators of vitamin D supply (plasma level of 25(OH)D and osteocalcin) to the level in control animals and animals with all the missing vitamins added to the feed. Without correcting the deficiency of В vitamins, the addition of vitamin D did not eliminate the disturbances in calcium metabolism (increased levels in the blood plasma by 7.9% and in the brain by 14.5%, decreased urinary excretion by 47.8%). Against the background of a deficiency of vitamins of group B, increased blood levels of glucose, cholesterol, urea, iron associated with vitamin D deficiency persisted. Conclusions. Deficiency of В vitamins interferes with the restoration of adeguate vitamin D sufficiency. The expediency of the combined use of vitamin D with В vitamins has been substantiated.

combined deficiency of vitamins D and B group, correction of vitamin D deficiency, blood plasma, liver, brain, urine, rats