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Vol 24, No 4 (2021)


Perspectives for production plant biomass biologically active product by the microbial fermentation method (review)

Khaibullin R.G., Volkova L.V.


Currently, under the various adverse factors influence, metabolic disorders are increasingly occurring in the population. It can lead to various chronic diseases, to the occurrence of a pre-diabetic state and non-insulin dependent diabetes (type 2 diabetes). As a means of these disorders occurrence preventing, herbal preparations are used due to their milder effect on the body, low toxicity and pronounced hypoglycemic effect of some of them. The fermentation method is widely used in their manufacture, and therefore, increasing of this method efficiency is an urgent task of biotechnology and pharmaceuticals. Literature data studying and analysis about using fermentation method in the plant drugs and dietary supplements production. On the presented review basis, a method for increasing the traditional fermentation efficiency by using Lactobacillus probiotic microorganisms is proposed. It makes it possible to enrich the product with the lactobacteria metabolism substances and obtain a product that has a synergistic effect on the human body.
Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):3-8
pages 3-8 views

Assessment of bile activity of multicomponent plant extract «PENTAFIT»

Ferubko E.V., Nikolaev S.M., Dargaeva T.D.


In the structure of morbidity and mortality of the population, liver lesions occupy a leading place due to an increase in the number of uncontrolled large-scale use of drugs, pollution of the environment with chemical compounds, alcoholic intoxications. Promising for the development of pharmacological correction methods for these conditions are multicomponent agents of plant origin, characterized by the breadth of therapeutic action, low toxicity and associated long-term use without the risk of developing side reactions. The advantage of multicomponent drugs is the mutual enhancement of the beneficial pharmacological properties of each incoming ingredient, the correspondence of the multivalence of the pathogenesis of the disease, the effect on the patient's body as a whole. The article presents the results of studying the bile activity of the multicomponent plant extract, under the conditional name «Pentafit»obtained from the following types of plant raw materials: of the roots and rhizomes of Inula helenium L. - 250 g, the herb of Centaurium ery-thraea Rafn. -150 g, theflowers of Tanacetum vulgare L. -100 g, thefruits of Rosa sp. - 275 g, the fruits of Crataegus sp. 225 g under carbon tetrachloride hepatitis model conditions. It was found that course administration per os «Pentafit» at a dose of 300 mg/kg to white nonlinear rats with carbon tetrachloride liver damage increases simultaneously the bile and bile function of the liver in animals. The bile effect of the extract in toxic liver damage is due to the presence in it of a complex of biologically active substances and, above all, phenolic compounds. The obtained research results argue the feasibility of using a multicomponent plant extract «Pentafit» containing biologically active substances of phenolic nature, in the complex treatment and prevention of liver diseases.
Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):9-14
pages 9-14 views

Determination of residual organic solvents in 2-benzoylamino-n-[4-(4,6-dimethylpyrimidin-2- ylsulfamoil)-phenyl]-benzamide by gas chromatography

Borovskiy B.V., Koval’ N.O., Kompancev V.A., Kodonidi I.P., Larskiy M.V., Rasskazov A.G.


Relevance. Due to the active growth of the pharmaceutical industry in the Russian Federation, one of the important tasks is quality control and standardization of manufactured pharmaceutical products. During the synthesis of pharmaceutical substances, both in laboratory conditions and at large pharmaceutical enterprises, various organic solvents are used. In accordance with the requirements of ND, the determination of the content of organic solvents is a mandatory requirement in the quality control of medicinal products, thus, the development of methods for the determination of residual organic solvents is relevant for modern pharmaceutical science. The aim of the study. To develop a method for the quantitative determination of residual organic solvents in the pharmaceutical substance of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsulfamoyl)-phenyl]-benzamide Material and methods. Investigation was carried out using gas chromatograph "Kristall 2000M" on 5 laboratory series of the substance of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsulfamoyl)-phenyl]-benzamide. For the analysis of acetic acid, a packed glass column FIP-FFAP was used (length 1 m, diameter. Stationary liquid phase - FFAP in an amount of 15% of the solid support, particle size 0.255 - 0.350 mm, acetic acid peak retention time 4.5. For the analysis of the residual DMF content, we used chromatographic column M (type 11) "Chromatek" made of stainless steel with a length of 2 m and an inner diameter of 3 mm. FFAP was used as a stationary liquid phase (in an amount of 10% of the solid carrier, particle size from 0.255 to 0.350 mm, retention time of the DMF peak 5.55 min, in both cases headspace analysis was used. Results. It was found that the average content of residual solvents per 1 g of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsamoyl)-phenyl]-benzamide is 34.59±1.02 mg for acetic acid and 38.99±1.70 mg for dimethylformamide. Proposed method has sufficient validation characteristics as linearity, precision and trueness. Conclusions. The proposed method makes it possible to determine the residual solvents in the substance of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsulfamoyl)-phenyl]-benzamide and can be included in the draft regulatory document for its pharmaceutical substance.
Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):15-23
pages 15-23 views

The preclinical study on the safety of adaptogenic herbal remedy

Razuvaeva Y.G., Toropova A.A., Banzaraksheev V.G., Ayusheeva V.V.


The purpose of this work is to determine chronic toxicity, as well as the possible local irritating and mutagenic effect of a complex herbal remedy. Material and methods. The studies were performed on white Wistar rats. The effect of an extract of a dry complex herbal remedy on the morphofunctional state of internal organs was assessed after its three-month administration {per os) at doses of 100 and 500 mg/kg. The possible locally irritating and mutagenic effect of the investigational agent was determined with its single administration at doses of 100 and 1000 mg/kg. Results. It was found that long-term administration of the investigational agent promotes an increase in the research activity of animals, exhibits a diuretic effect, and also reduces the level of cholesterol, the content of urea and creatinine in the serum of white rats. A single administration of the test agent in doses of 100 and 1000 mg/kg does not have a local irritating effect on the mucous membrane of the digestive tract and does not exhibit a mutagenic effect. Conclusions. The investigated agent, with long-term administration, does not have a toxic effect on the morphofunctional state of internal organs, with a single administration-locally irritating and mutagenic effects.
Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):24-29
pages 24-29 views

Dependence of the efficiency of vitamin D deficiency correction and its consequences in rats from supply with B group vitamins

Kodentsova V.M., Leonenko S.N., Beketova N.A., Kosheleva O.V., Vrzhesinskaya O.A., Sokolnikov A.A., Shevyakova L.V., Risnik D.V., Zorin S.N.


Relevance. Multiple micronutrient deficiencies, the most freguent of which is a lack of vitamin D, then В vitamins, is typical for the population of Russia. Taking cholecalciferol without prescribing В vitamins, which are necessary for its transformation into the hormonal form, is recommended for correcting vitamin D deficiency The aim of the study is to compare the effectiveness of vitamin D deficiency correction in rats against the background of adeguate and insufficient supply of В vitamins. Material and methods. The combined deficiency of vitamins D and group В in male Wistar rats (n = 33) with body weight (69.5 ± 0.8 g) was caused d by a 5-fold decrease in the amount of vitamins in the vitamin mixture of a semi-synthetic diet for 23 days. During the next 7 days, in order to correct vitamin deficiency, 12 rats (group «-В +D») received a diet supplemented to 100% in vitamin D against the background of continuing deficiency of vitamins of group B, and 12 rats (group «+В +D») - a diet supplemented with all the missing vitamins. Animals of the control group (n = 9) received a full-fledged semi-synthetic diet. The content of calcium and iron in the freeze-dried liver and brain was determined on a Z 5300 atomic absorption spectrophotometer (Hitachi High-Technologies Corporation (HHC), Japan), biochemical parameters of blood and urine were determined on a KoneLab 200І biochemical analyzer (ThermoScientific, Finland). Results. Against the background of a deficiency of В vitamins, the addition of cholecalciferol to rats deficient in В and D vitamins did not fully restore the indicators of vitamin D supply (plasma level of 25(OH)D and osteocalcin) to the level in control animals and animals with all the missing vitamins added to the feed. Without correcting the deficiency of В vitamins, the addition of vitamin D did not eliminate the disturbances in calcium metabolism (increased levels in the blood plasma by 7.9% and in the brain by 14.5%, decreased urinary excretion by 47.8%). Against the background of a deficiency of vitamins of group B, increased blood levels of glucose, cholesterol, urea, iron associated with vitamin D deficiency persisted. Conclusions. Deficiency of В vitamins interferes with the restoration of adeguate vitamin D sufficiency. The expediency of the combined use of vitamin D with В vitamins has been substantiated.
Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):30-37
pages 30-37 views

Development of ethanol-induced working memory impairment model in Wistar rats: behavioral and neurochemical studies

Konkov V.G., Kasabov K.A., Naplekova P.L., Narkevich V.B., Kudrin V.S., Kolik L.G.


Introduction. Alcohol withdrawal is an important factor that may lead to cognitive impairment. It is known that an alcohol withdrawal leads to significant memory and learning deficits in rats in various tests. Taking into account the need to develop novel ways of pharmacological correction of alcohol dependence, the goal of this work was to develop and validate an experimental model of alcohol induced impairments associated with the function of non-spatial hippocampal-dependent working memory. Material and methods. The experiments were performed on mature male Wistar rats weighing 275-300 g. (n = 16) Wistar rats were randomly divided into control (n=8) and experimental (n=8) groups. Animals from the experimental group were injected with ethanol at intervals of 2 hours at a dose of 3 g / kg per day, intragastrically, for 7 days, animals from the control group received distilled water in the same mode at the rate of 0.1 ml/100 g of body weight. 24 hours after the last injection of the ethanol solution, the animals behavior was evaluated in the "new object recognition" test. After completing behavioral studies, the animals were decapitated, brain structures (anterior cingulate cortex, insular cortex, nucleus accumbens, and hippocampus) were extracted on ice, and frozen in liguid nitrogen. The determination of neurotransmitter monoamines and amino acids was performed by HPLC. Results. There was a decrease in dopamine turnover in the hippocampus and anterior cingulate cortex, a decrease in the activity of the serotonergic system in the insular cortex. Conclusion. Alcohol at a dose of 3 g / kg with subchronic administration per os impairs the working non-spatial memory of Wistar rats.
Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):38-44
pages 38-44 views

The relationship of physical activity with the indicators of zinc and selenium metabolism in experimental animals

Skalny A.A.


The aim of the study. To study the effect of physical activity and additional administration of zinc on the level of zinc and selenium in rats. Material and Methods. The study was performed on 32 males of the Wistar line were divided into four groups: a control group, exposed to physical activity, receiving zinc (15 mg/kg of weight in the form of zinc asparaginate), exposed to physical activity and receiving zinc. The content of zinc and selenium in organs and tissues was evaluated by inductively coupled plasma mass spectrometry (ICP-MS). Results. Physical activity significantly reduced the concentration of zinc in skeletal muscle, kidneys, and wool, and selenium in the liver, heart muscle, skeletal muscle, and kidneys of rats. At the same time, physical activity led to an increase in the content of zinc in the liver, and selenium - in the blood serum and hair of rats. The additional administration of zinc reduced the increased zinc content in the liver, kidneys, heart muscle, and fur in the rats exposed to physical exertion. With the additional introduction of zinc, selenium reserves began to increase in the group of animals with physical activity. Conclusions. The additional administration of zinc had a positive effect on the body of rats during physical exertion. This may be due not only to changes in zinc metabolism, but also to the regulation of selenium metabolism, as well as their biological effects.
Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):45-50
pages 45-50 views

Vitamin D availability in therapeutic and dental patients

Boldyreva Y.V., Kurlovich N.A., Lebedev I.A., Bragin A.V., Shikhshabekova W.A., Zaitkhanova K.E.


In the world, the participation of D3 in biochemical processes is being studied. It is proved that the role of D3 is not only the regulation of calcium-phosphorus metabolism. The paper studied the level of D3 of patients observed in one of the polyclinics in Tyumen; 730 medical records were studied. To evaluate and analyze the relationship between the level of D3 among patients of therapeutic and dental profile, observed in the consultative and diagnostic polyclinic named After E. M. Niginsky in Tyumen with: age and gender, place of residence, reason for seek ing medical care, the presence of concomitant pathology. The D3 content in the blood was determined by the immunological method of guantitative determination in vitro. We used the method of enzyme immunoassay using the 25-OH Vitamin D ELISA kit (Euroimmun, Germany). Most individuals have a D3 deficiency, which can be the result of many reasons. These individuals are a risk group for the development of pathology of processes controlled by D3. It should be remembered that every doctor can face them. In this regard, the issues of di agnosis and prevention of D3 deficiency should be known by doctors of all specialties.
Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):51-54
pages 51-54 views

Nikolay L'vovich Shimanovskiy (k 70-letiyu so dnya rozhdeniya)

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Problems of Biological Medical and Pharmaceutical Chemistry. 2021;24(4):55-55
pages 55-55 views

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