Vol 24, No 4 (2021)

Currently, under the various adverse factors influence, metabolic disorders are increasingly occurring in the population. It can lead to various chronic diseases, to the occurrence of a pre-diabetic state and non-insulin dependent diabetes (type 2 diabetes). As a means of these disorders occurrence preventing, herbal preparations are used due to their milder effect on the body, low toxicity and pronounced hypoglycemic effect of some of them. The fermentation method is widely used in their manufacture, and therefore, increasing of this method efficiency is an urgent task of biotechnology and pharmaceuticals. Literature data studying and analysis about using fermentation method in the plant drugs and dietary supplements production. On the presented review basis, a method for increasing the traditional fermentation efficiency by using Lactobacillus probiotic microorganisms is proposed. It makes it possible to enrich the product with the lactobacteria metabolism substances and obtain a product that has a synergistic effect on the human body.

Relevance. Due to the active growth of the pharmaceutical industry in the Russian Federation, one of the important tasks is quality control and standardization of manufactured pharmaceutical products. During the synthesis of pharmaceutical substances, both in laboratory conditions and at large pharmaceutical enterprises, various organic solvents are used. In accordance with the requirements of ND, the determination of the content of organic solvents is a mandatory requirement in the quality control of medicinal products, thus, the development of methods for the determination of residual organic solvents is relevant for modern pharmaceutical science. The aim of the study. To develop a method for the quantitative determination of residual organic solvents in the pharmaceutical substance of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsulfamoyl)-phenyl]-benzamide Material and methods. Investigation was carried out using gas chromatograph "Kristall 2000M" on 5 laboratory series of the substance of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsulfamoyl)-phenyl]-benzamide. For the analysis of acetic acid, a packed glass column FIP-FFAP was used (length 1 m, diameter. Stationary liquid phase - FFAP in an amount of 15% of the solid support, particle size 0.255 - 0.350 mm, acetic acid peak retention time 4.5. For the analysis of the residual DMF content, we used chromatographic column M (type 11) "Chromatek" made of stainless steel with a length of 2 m and an inner diameter of 3 mm. FFAP was used as a stationary liquid phase (in an amount of 10% of the solid carrier, particle size from 0.255 to 0.350 mm, retention time of the DMF peak 5.55 min, in both cases headspace analysis was used. Results. It was found that the average content of residual solvents per 1 g of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsamoyl)-phenyl]-benzamide is 34.59±1.02 mg for acetic acid and 38.99±1.70 mg for dimethylformamide. Proposed method has sufficient validation characteristics as linearity, precision and trueness. Conclusions. The proposed method makes it possible to determine the residual solvents in the substance of 2-benzoylamino-N-[4-(4,6-dimethylpyrimidin-2-ylsulfamoyl)-phenyl]-benzamide and can be included in the draft regulatory document for its pharmaceutical substance.

Relevance. Multiple micronutrient deficiencies, the most freguent of which is a lack of vitamin D, then В vitamins, is typical for the population of Russia. Taking cholecalciferol without prescribing В vitamins, which are necessary for its transformation into the hormonal form, is recommended for correcting vitamin D deficiency The aim of the study is to compare the effectiveness of vitamin D deficiency correction in rats against the background of adeguate and insufficient supply of В vitamins. Material and methods. The combined deficiency of vitamins D and group В in male Wistar rats (n = 33) with body weight (69.5 ± 0.8 g) was caused d by a 5-fold decrease in the amount of vitamins in the vitamin mixture of a semi-synthetic diet for 23 days. During the next 7 days, in order to correct vitamin deficiency, 12 rats (group «-В +D») received a diet supplemented to 100% in vitamin D against the background of continuing deficiency of vitamins of group B, and 12 rats (group «+В +D») - a diet supplemented with all the missing vitamins. Animals of the control group (n = 9) received a full-fledged semi-synthetic diet. The content of calcium and iron in the freeze-dried liver and brain was determined on a Z 5300 atomic absorption spectrophotometer (Hitachi High-Technologies Corporation (HHC), Japan), biochemical parameters of blood and urine were determined on a KoneLab 200І biochemical analyzer (ThermoScientific, Finland). Results. Against the background of a deficiency of В vitamins, the addition of cholecalciferol to rats deficient in В and D vitamins did not fully restore the indicators of vitamin D supply (plasma level of 25(OH)D and osteocalcin) to the level in control animals and animals with all the missing vitamins added to the feed. Without correcting the deficiency of В vitamins, the addition of vitamin D did not eliminate the disturbances in calcium metabolism (increased levels in the blood plasma by 7.9% and in the brain by 14.5%, decreased urinary excretion by 47.8%). Against the background of a deficiency of vitamins of group B, increased blood levels of glucose, cholesterol, urea, iron associated with vitamin D deficiency persisted. Conclusions. Deficiency of В vitamins interferes with the restoration of adeguate vitamin D sufficiency. The expediency of the combined use of vitamin D with В vitamins has been substantiated.

The aim of the study. To study the effect of physical activity and additional administration of zinc on the level of zinc and selenium in rats. Material and Methods. The study was performed on 32 males of the Wistar line were divided into four groups: a control group, exposed to physical activity, receiving zinc (15 mg/kg of weight in the form of zinc asparaginate), exposed to physical activity and receiving zinc. The content of zinc and selenium in organs and tissues was evaluated by inductively coupled plasma mass spectrometry (ICP-MS). Results. Physical activity significantly reduced the concentration of zinc in skeletal muscle, kidneys, and wool, and selenium in the liver, heart muscle, skeletal muscle, and kidneys of rats. At the same time, physical activity led to an increase in the content of zinc in the liver, and selenium - in the blood serum and hair of rats. The additional administration of zinc reduced the increased zinc content in the liver, kidneys, heart muscle, and fur in the rats exposed to physical exertion. With the additional introduction of zinc, selenium reserves began to increase in the group of animals with physical activity. Conclusions. The additional administration of zinc had a positive effect on the body of rats during physical exertion. This may be due not only to changes in zinc metabolism, but also to the regulation of selenium metabolism, as well as their biological effects.