Determination of total triterpene saponins in a combined drug based on jacob’s ladder, motherwort herbs and hawthorn fruit by spectrophotometry
- Authors: Hajjar F.1, Potanina O.G1
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Affiliations:
- People's Friendship University of Russia
- Issue: Vol 24, No 12 (2021)
- Pages: 41-45
- Section: Articles
- URL: https://journals.eco-vector.com/1560-9596/article/view/112916
- DOI: https://doi.org/10.29296/25877313-2021-12-06
- ID: 112916
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Abstract
Purpose: The purpose was to evaluate analytical procedures for the quantitative determination of the total triterpene saponins in a combined herbal medicine based on motherwort herb, hawthorn fruit and jacob's ladder herb by ultraviolet-visible spectrophotometry. The article presents determining the amount of triterpene saponins in a combined sedative drug based on spissum extracts of pharma-copoeial medicinal plant raw materials of motherwort herb, hawthorn fruits and jacob's ladder herb, using spectrophotometry. In according to requirements of state pharmacopoeia th14, General Pharmacopoeia Monograph.1.2.1.1.0003.15 "Spectrophotometry in the UV and visible regions" using a spectrophotometric method for determining the content of the sum of triterpene saponins in terms of β-escin. The conditions for the analysis were taken from the monograph 1.2.5.0039.15 of state pharmacopoeia th14. The method was validated according to requirements of General Pharmacopoeia Monograph 1.1.0012.15 "Validation of analytical methods" in terms of the parameters: specificity, linearity, correctness, repeatability and precision of solutions. The data obtained can be used in the development of regulatory documents for the developed combined drug in the form of a spissum extract.
Keywords
Herba Polemonii, Polemonium caeruleum L, Herba Leonuri, Leonurus quinquelobatus Gilib, Leonurus cardiac L, Fructus Crataegi, Crataegus spp, Herba Polemonii, Polemonium caeruleum L, Herba Leonuri, Leonurus quinquelobatus Gilib, Leonurus cardiac L, Fructus Crataegi, Crataegus spp, jacob's ladder, herba leonuri, Hawthorn, spissum extract, triterpene saponins, UV-spectrophotometry
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About the authors
Fadi Hajjar
People's Friendship University of Russia
Email: phfadyhajj@gmail.com
Post-gratuate Student
O. G Potanina
People's Friendship University of RussiaDr.Sc. (Pharm.), Professor
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