The effect of the hormonal composition of the nutrient medium and endogenous polyphenols on the formation of callus tissue Ipomoea batatas (L.)
- Authors: Kalashnikova E.A.1, Kirakosyan R.N.1, Abubakarov H.G.1, Zaitseva S.M.1
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Affiliations:
- Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazeva
- Issue: Vol 25, No 11 (2022)
- Pages: 46-58
- Section: Articles
- URL: https://journals.eco-vector.com/1560-9596/article/view/113010
- DOI: https://doi.org/10.29296/25877313-2022-11-08
- ID: 113010
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Abstract
Relevance. Ipomoea batatas (L.) is a valuable agricultural crop, in the tubers of which inulin accumulates, a natural polysaccharide that has no synthetic analogues. Market analysis shows that, as a rule, in the production of functional food products, Russian manufacturers use imported inulin. Currently, globalization, the information revolution, as well as the sanctions policy have exacerbated significant problems of the world economy, which increases the relevance of import substitution of a number of goods and products. It is known that cold stress is one of the main environmental factors limiting agricultural production. Low positive temperatures have a negative impact on the growth, development, productivity and yield of I. batatas (L.) plants. It is possible to create such plants using cell biotechnology methods, in particular, in vitro cell selection, which is carried out on a callus culture.
Purpose of the study. To study the effect of the hormonal composition of the nutrient medium and endogenous polyphenols on the formation of the callus tissue of sweet potatoes (I. batatas (L.)) in vitro.
Material and methods. The object of the study were three varieties of I. batatas (L.) (Purple, Jewel, Porto Rico). Callus tissue was obtained from segments of leaf blades and stem internodes, which were isolated from aseptic sweet potato plants. Explants were cultured on MS nutrient medium containing BAP 0.5 mg/l and 1 mg/l NAA/IAA/2,4-D. The localization of phenolic compounds was studied in leaves, stems, and apical buds of microclones. In addition, localization was studied in the callus tissue obtained on different auxins. Histochemical methods were used for this. For the amount of phenolic compounds, the material was stained with 0.08% Fast Blue reagent raster. To study the localization of flavans (catechins and proanthocyanidins), a reaction with a vanillin reagent in hydrochloric acid vapors was used.
Results. It was found that the auxins used had a significant effect on the intensity of callus tissue formation, its consistency and color. A well-proliferating callus tissue of light yellow color was obtained on a medium with NAA, a dense, green rhizogenic callus tissue was formed on a medium with IAA, and a dark brown tissue was formed on a medium with 2,4-D, which died during cultivation. As a rule, callus tissue was formed in those places where the formation and localization of phenolic compounds was insignificant.
Conclusion. It was found that in callus cultures initiated from leaf plates and grown on a nutrient medium with NAA, the content of cells with phenolic compounds was less than that of callus obtained from leaf plates as well, but on a medium with 2,4-D.
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About the authors
E. A. Kalashnikova
Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazeva
Author for correspondence.
Email: kalash0407@mail.ru
Dr.Sc. (Biol.), Professor, Department of Biotechnology
Russian Federation, MoscowR. N. Kirakosyan
Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazeva
Email: mia41291@mail.ru
Ph.D. (Biol.), Associate Professor, Department of Biotechnology
Russian Federation, MoscowH. G. Abubakarov
Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazeva
Email: khrpo95@mail.ru
Post-graduate Student, Associate Professor, Department of Biotechnology
Russian Federation, MoscowS. M. Zaitseva
Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazeva
Email: smzaytseva@yandex.ru
Ph.D. (Biol.), Associate Professor, Department of Biotechnology
Russian Federation, MoscowReferences
- Franck A. Technological functionality of inulin and oligofructose. Br. J. Nutr. 2002; 87: S287–S291.
- Kumar J., Rani K., Datt C. Molecular link between dietary fibre, gut microbiota and health. Mol. Biol. Rep. 2020; 47: 6229–6237.
- Sabater-Molina M., Larqué E., Torrella F., Zamora S. Dietary fructooligosaccharides and potential benefits on health. J. Physiol. Biochem. 2009; 65: 315–328.
- Ahmed W., Rashid S. Functional and therapeutic potential of inulin: A comprehensive review. Crit. Rev. Food Sci. Nutr. 2019; 59: 1–13.
- Saeed F., Pasha I., Arshad M.U., Anjum F.M., Hussain S., Rasheed R., Nasir M.A., Shafique B. Physiological and nutraceutical perspectives of fructan. Int. J. Food Prop. 2015; 18: 1895–1904.
- Shoaib M., Shehzad A., Omar M., Rakha A., Raza H., Sharif H.R., Shakeel A., Ansari A., Niazi S. Inulin: Properties, health benefits and food applications. Carbohydr. Polym. 2016; 147: 444–454.
- Shang H.M., Zhou H.Z., Yang J.Y., Li R., Song H., Wu H.X. In vitro and in vivo antioxidant activities of inulin. PLoS ONE. 2018; 13: 19–22.
- Namanda S., Gibson R.W., Kirimi S. Sweet potato seed systems in Uganda, Tanzania and Rwanda. Journal of Sustainable Agriculture. 2011; 35: 870–884.
- Ogero K.O., Gitonga N.M., Mwangi M., Ombori O., Ngugi M. A low-cost medium for sweet potato micro propagation. African Crop Science Conference Proceedings. 2011; 10: 57–63.
- Chaum S., Kirdmanee C. Diseases free production of sugarcane varieties (Saccharum officinarum L.) using in vitro meristem culture. Biotechnology. 2006; 5(4): 443–448.
- Doliñski R., Olek O. Micropropagation of sweet potato (Ipomoea batatas (L.) Lam.) from node explants. Acta Sci Pol., Hortorum Cultus. 2013; 12(4): 117–127.
- Liu Q.C., Zhai H., Wang Y., Zhang D.P. Efficient plant regeneration from embryonic suspension cultures of sweet potato. In Vitro Cell Developmental Biology-Plant. 2001; 37: 564–567.
- Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962; 15: 473–497.
- Soukupova J., Cvikrova M., Albrechtova J., Rock B.N., Eder J. Histochemical and Biochemical Approaches to the Study of Phenolic Compounds and Peroxidases in Needles of Norway Spruce (Picea abies). New Phytol. 2000; 146: 403–414.
- Lakin G.F. Biometrija: ucheb. posobie dlja biol. spec. vuzov. M.: Vysshaja shkola. 1990. 352 s.
- Dubravina G.A., Zajceva S.M., Zagoskina N.V. Izmenenija v obrazovanii i lokalizacii fenol'nyh soedinenij pri dedifferenciacii tkanej tissa jagodnogo i tissa kanadskogo v uslovijah in vitro. Fiziologija rastenij. 2005; 52: 755–762.
- Zaprometov M.N. Fenol'nye soedinenija i ih rol' v zhizni rastenija. LVI Timirjazevskie chtenija. M.: Nauka. 1996. 45 s.
- Tulupova E.S., Ostrozhenkova E.G., Slepjan., Sakanjan E.I. Vlijanie razlichnyh citokininov na rost selektivnyh shtammov Panax ginseng S.A.Meu i Panax quinquefolius L. s germatranom Lx 5 i soderzhanie v nih glikozidov. Biotehnologija. 2002; 3: 30–36.
- Kalashnikova, E.A., Zajceva S.M., Doan Thu Thui, Kirakosjan R.N. Vlijanie reguljatorov rosta na morfogeneticheskuju aktivnost' jeksplanotov Dioscorea nipponica Makino i obrazovanie polifenolov. Mezhdunarodnyj nauchno-issledovatel'skij zhurnal. 2020; 6-2(96): 6–11.
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