Essential oils content in sage medicinal plants as affected by artificial light spectral composition

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Relevance. Sage is a valuable medicinal plant. To obtain ecologically safe products on the background of environmental pollution, as well as climatic zoning restrictions in outdoor cultivation, cultivation of plants can be organized in the controlled environment with artificial lighting. In this case, application of the light-emitting diodes (LEDs) and high-pressure sodium (HPS) lamps requires the development of special light-growing regimes ("light recipes").

The aim of the research. To study the effects of different spectral composition on the qualitative and quantitative composition of sage essential oil components to optimize its cultivation in the controlled environment.

Material and methods. Plants of common sage (Salvia officinalis L.) variety Kubanets were grown in a vegetative experiment under controlled conditions with artificial lighting in the absence of natural light. HPS lamp (reference treatment), white LEDs, as well as narrow-band LEDs with radiation in red and blue regions of PAR were used in the experiment. The amount and component composition of the essential oil in the plant biomass were studied by gas chromatography-mass spectrometry (GC-MS).

Results. Evaluation of the qualitative and quantitative composition of sage extractive substances showed significant differences in the dynamics of component accumulation during the observation period. Thus, the content of 1,8-cineole increased from day 58 to day 110, and the content of α- and β-tuon decreased (exception: the treatment with red light). The content of camphor from day 58 to 110 decreased under all sources of irradiation.

Conclusions. The qualitative and quantitative composition of the target essential oil components depends not only on the age of sage plants, but also depends on the spectral composition of light sources. Along with high operating and economic indicators of LEDs (long life, low heat dissipation, reduced energy costs per unit of biomass), it allows to increase the profitability of the basil cropping in the controlled environment, in particular - when growing in vertical farms.

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作者简介

A. Ivanitskikh

Federal Scientific Agroengineering Center VIM

编辑信件的主要联系方式.
Email: alinena@yandex.ru

Junior Research Scientist

俄罗斯联邦, Moscow

I. Tarakanov

Russian State Agrarian University – Moscow Timiryazev Agricultural Academy

Email: alinena@yandex.ru

Dr.Sc. (Biol.), Professor, Department of Plant Physiology, Institute of Agrobiotechnologies

俄罗斯联邦, Moscow

参考

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  2. Perry N., Anderson R., Brennan N., Douglas M., Heaney A., McGimpsey J., Smallfield B. Essential oils from Dalmatian sage (Salvia officinalis L.): variations among individuals, plant parts, seasons, and sites. Agric Food Chem. 1999; 47(5): 2048–2054.
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  4. Croteau R., Karp F. Biosynthesis of monoterpenes: hydrolysis of bornyl pyrophosphate, an essential step in camphor biosynthesis, and hydrolysis of geranyl pyrophosphate, the acyclic precursor of camphor, by enzymes from sage (Salvia officinalis). Arch Biochem Biophys. 1979; 198(2): 523–532.
  5. Croteau R., Karp F. Biosynthesis of monoterpenes: preliminary characterization of bornyl pyrophosphate synthetase from sage (Salvia officinalis) and demonstration that Geranyl pyrophosphate is the preferred substrate for cyclization. Arch Biochem Biophys. 1979 б; 198(2): 512–522.
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2. Fig. 1. Spectra of optical radiation of the irradiators used in the experiment: a – LLD, b – SDb, c – SDk+s, d – SDk

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3. Fig. 2. Sage plants of the Kubanets variety at the age of 58 days in the cultivation options: 1 - SDK, 3 - NLVD, 4 - SDB, 5 - SDK + s

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4. Fig. 3. Chromatogram with the release times of components of the essential oil of sage officinalis grown under irradiation with SDK + s at the age of 110 days: 4.411 - α-pinene, 4.625 - camphene, 5.011 - β-pinene, 5.135 - β-myrcene, 5.723 - limonene, 5.775 - 1,8-cineol (eucalyptol), 6.592 - linalool, 6.873 - α-thujone, 7.028 - β-thujone, 7.474 - camphor, 7.765 - borneol, 9.451 - bornyl acetate, 9.732 - pinocarviol acetate, 10.211 - bourbonene, 11. 316 - caryophyllene , 11.479 - veridiflorol, 11.680 - humulene, 11.753 - eicosan, 12.785 - silinene, 13.334 - hexadecane (internal standard), 13.460 - humulene oxide

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