Effect of modulation of nitrogen oxide (II) synthesis under chronic normobaric hypoxia on the isoenzyme spectrum of rat epididimis lactate dehydrogenase

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Abstract

Lactate dehydrogenase have a key role in providing energy to cells under physiological and hypoxic conditions. Changes in the activity of the enzyme can be facilitated by nitric oxide (II), regulating the expression of individual fractions of lactate dehydrogenase.

Aim. To study changes in the isoenzyme spectrum of rat epididymis lactate dehydrogenase under conditions of hypoxia and modulation of nitric oxide (II) synthesis.

Material and methods. Male rats (32) were divided into 4 groups (n=8): 1) chronic normobaric hypoxia; 2) control to group 1; 3) hypoxia together with modeling of nitric oxide deficiency; 4) hypoxia against the background of induction of NO synthesis. The mitochondrial fraction and non-mitochondrial cytoplasm of the head and tail of the epididymis were isolated for laboratory research. The zymogram was obtained by electrophoresis in 7% gel followed by detection by the precipitation reaction of the reduction of nitro blue tetrazolium. The overall activity of lactate dehydrogenase was assessed and the percentage of isoenzyme fractions was calculated. Fractions 1 and X of lactate dehydrogenase were analyzed, the results were considered statistically significant if p<0.05 when comparing two independent samples, and p<0.0167 when comparing three independent samples.

Results. Chronic normobaric hypoxia leads to a decrease in lactate dehydrogenase activity compared to the control group. At the same time, the share of the X-fraction of the enzyme and lactate dehydrogenase 1 increases. Modulation of nitric oxide (II) deficiency against the background of hypoxia increased the activity of the enzyme in the head of the epididymis, where a decrease in the studied fractions of lactate dehydrogenase was observed compared to the group of animals subjected only to hypoxia. Stimulation of the synthesis of nitric oxide (II) during hypoxia led to a decrease in the X-fraction, but an increase in the 1st fraction of lactate dehydrogenase in the head of the epididymis, in the tail of the epididymis, opposite changes were observed.

Conclusion. Hypoxia causes a change in the ratio of lactate dehydrogenase isoenzymes towards an increase in lactate dehydrogenase B and lactate dehydrogenase X, while a deficiency of nitric oxide (II) contributes to a decrease in lactate dehydrogenase X synthesis.

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About the authors

Y. A. Marsyanova

Ryazan State Medical University named after academician I.P. Pavlov

Author for correspondence.
Email: yuliyamarsyanova@yahoo.com
ORCID iD: 0000-0003-4948-4504
SPIN-code: 4075-3169

Assistant, Department of Biological Chemistry with the Course of CLD FAPE

Russian Federation, Ryazan

V. I. Zvyagina

Ryazan State Medical University named after Academician I.P. Pavlov

Email: vizvyagina@yandex.ru

Ph.D., Associate Professor, Department of Biological Chemistry with the Course of CLD FAPE

Russian Federation, Ryazan

D. A. Solovykh

Ryazan State Medical University named after Academician I.P. Pavlov

Email: solovykh1234@mail.ru

Student

Russian Federation, Ryazan

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2. Figure. An example of a lactate dehydrogenase zymogram; the separation of fractions was carried out under conditions of non-denaturing electrophoresis in 7% PAAG; the coloration was obtained in the reaction of electron transfer from the substrate-donor sodium lactate to the substrate-acceptor nitrosine tetrazolium, catalyzed by LDH; on the right - the scale in% and graphical interpretation based on the calculation of the optical density of each individual fraction using the program "Microsoft Office Excel 2013"

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