Biological features of Hedysarum alpinum L. cell culture when grown in vitro
- Authors: Savin P.S.1, Tsybulko N.S.1, Myasnikova S.B.1
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Affiliations:
- All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
- Issue: Vol 27, No 1 (2024)
- Pages: 62-68
- Section: Biological chemistry
- URL: https://journals.eco-vector.com/1560-9596/article/view/625795
- DOI: https://doi.org/10.29296/25877313-2024-01-08
- ID: 625795
Cite item
Abstract
The purpose of the research conducted was to obtain a cell culture of Hedysarum alpicum, as a producer of xanthone mangiferin, by achieving the tasks of obtaining a cell culture of H. alpium growing in deep-cultivation conditions and studying its physiological characteristics.
Material and methods. The object of the study was a cell culture of H. alpine obtained from the cotyledon of the germination, strain H. alpinum (C) 2016 of the collection of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants» and the native plant grown on the pharmacopeial site of the Botanical Garden of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants», harvest of 2013.
Results. It has been established that growth regulators kinetin and 2.4D stimulate growth processes of H. alpium (C) cell culture 2016 in deep cultivation conditions. The duration of growth is 20–23 days. The maximum increase corresponds to 16 g/l on the 20th day of growth. The maximum consumption of oxygen by the cell culture is 30·10-4 mol/l·h on the 16th day of growth, when the cells are in the regular growth phase.
Conclusion. As a result of the work carried out, a stable cell strain of H. alpium was obtained, growing on the surface of the agarized nutrient medium, which has the potential for the synthesis of mangiferin and work has been carried out on studying its physiological characteristics.
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About the authors
P. S. Savin
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Author for correspondence.
Email: savin-pavel@list.ru
Ph.D. (Biol.)
Russian Federation, MoscowN. S. Tsybulko
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: savin-pavel@list.ru
Ph.D. (Pharm.)
Russian Federation, MoscowS. B. Myasnikova
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
Email: savin-pavel@list.ru
Research Scientist
Russian Federation, MoscowReferences
- Vichkanova S.A. Jeffektivnost' alpizarina pri gerpesvirusnyh zabolevanijah u detej i vzroslyh. Praktiches-kaja fitoterapija. 2000; 1: 34–39.
- Ljuboshenko T.M. Kliniko-immunologicheskaja harakteristika i lechenie pacientov s gerpeticheskoj infekciej. JeNI Zabajkal'skij medicinskij vestnik. 2015; 1: 17–23.
- Matkowski A. Kuś P., Góralska E., Woźniak D. Mangiferin – a bioactive xanthonoid, not only from mango and not just antioxidant. Mini Rev Med Chem. 2013; 13(3): 439–455.
- Muruganadan S., Gupta J., Lal P.K. Immunotherapeutic effect of mangiferin mediated by the inhibition of oxidative stress to activated lymphocytes neutrophilis and macrophages. Toxicology. 2005; 215: 57–68.
- Jagetia G.C., Venkatesha V.A. Mangiferin protects human peripheral blood lymphocytes against γ-radiation-induced DNA strand breaks: a fluorescence analysis of DNA unwinding assay. Nutrition Reserch. 2006; 26: 303–311.
- Gol'dberg E.D., Zueva E.P. Preparaty iz rastenij v kompleksnoj terapii zlokachestvennyh novoobrazovanij. Tomsk. 2000; 129 s.
- Lovkova M.Ja., Rabinovich A.M., Ponamoreva S.M., Buzuk G.N., Sokolova S.M. Pochemu rastenija lechat. M., 1989. 255 s.
- Tihonova E.D., Shemerjankina T.B., Sokol'skaja T.A. Alpizarin – sovremennyj preparat protivovirusnogo dejstvija, usovershenstvovanie tehnologii poluchenija preparata. Zhurnal nauchnyh statej «Zdorov'e i obrazovanie v XXI veke». 2008; 10(3): 395.
- Kukushkina T.A., Zinner N.S., Vysochina G.I., Sviridova T.P. Soderzhanie ksantonov v nadzemnoj chasti rastenij Hedysarum theinum krasnob. i H. alpinum L. (Fabaceae) pri vyrashhivanii v sibirskom botanicheskom sadu. Himija rastitel'nogo syr'ja. 2011; 3: 113–116.
- Gosudarstvennaja farmakopeja RF. XIII izd. M. 2015. T. 3. S. 402–403.
- Kunah V.A., Levenko B.A. Modifikacija metoda davlenyh preparatov dlja izuchenija hromosom v kletkah kul'tury tkanej rastenij. Citologija i genetika. 1975; 9(1): 56–58.
- Lebedev S.I. Fiziologija rastenij. M.: Agropromizdat, 1988. 544 s.
- Poljarograficheskoe opredelenie kisloroda v biologicheskih ob’ektah: materialy ІІ Vsesojuznogo simpoziuma. Kiev,1972. Kiev: Naukova dumka, 1974. 292 s.
- Malahova L.A., Amel'chenko V.P., Kataeva T.N. Citogeneticheskie issledovanija redkih rastenij tomskoj oblasti v SibBS – metodicheskaja osnova sohranenija ih bioraznoobrazija. Vestnik tomskogo gosudarstvennogo universiteta: Biologija. 2008; 2: 73–82.