Vol 27, No 1 (2024)

The purpose of the research conducted was to obtain a cell culture of Hedysarum alpicum, as a producer of xanthone mangiferin, by achieving the tasks of obtaining a cell culture of H. alpium growing in deep-cultivation conditions and studying its physiological characteristics.

Material and methods. The object of the study was a cell culture of H. alpine obtained from the cotyledon of the germination, strain H. alpinum (C) 2016 of the collection of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants» and the native plant grown on the pharmacopeial site of the Botanical Garden of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants», harvest of 2013.

Results. It has been established that growth regulators kinetin and 2.4D stimulate growth processes of H. alpium (C) cell culture 2016 in deep cultivation conditions. The duration of growth is 20–23 days. The maximum increase corresponds to 16 g/l on the 20^th day of growth. The maximum consumption of oxygen by the cell culture is 30·10^-4 mol/l·h on the 16^th day of growth, when the cells are in the regular growth phase.

Conclusion. As a result of the work carried out, a stable cell strain of H. alpium was obtained, growing on the surface of the agarized nutrient medium, which has the potential for the synthesis of mangiferin and work has been carried out on studying its physiological characteristics.

Introduction. The use of phenolic compounds as agents for preventing the risk of developing various cardiovascular diseases, as well as the prospect of creating new modern highly effective drugs based on them, is of current practical interest.

The aim of the study is to identify the hypolipidemic activity of the phytocomplex of the herb Phacelia tanacetifolia Benth., and the possibility of its use in herbal preparations for the treatment of diseases of the cardiovascular system.

Material and methods. The object of research was a herb of phacelia tanacetifolia, grown in the Neftekumsky region. The herb was collected during flowering and dried by air-shadowing method. The study of hypolipidemic activity was carried out on 24 white female Wistar rats weighing 270-290 g. In the experiment, a Tween model of hyperlipidemia was created and used: a single intraperitoneal injection of Tween-80 at a dose of 250 mg / 100 g of body weight. Lipid profile analysis was carried out on a biochemical analyzer RAL CLIMA MC-15 using reagents from DiaSys, manufactured in Germany.

Results. Based on the level of triglycerides and very low-density lipoproteins, it was found that the phytocomplex significantly exceeded the values of the reference drug (fenofibrate-canon).

Conclusions. In conditions of twin hyperlipidemia, therapeutic and prophylactic administration of the Phacelia tanacetifolia phytocomplex at a dose of 300 mg/kg helps to normalize blood serum lipid metabolism.

Relevance. In many countries, plants have long been used in folk medicine as a source of medicines, as they are well tolerated, gradual development of therapeutic effect and a mild effect on the body. Due to these features such medicines are being safely used in the treatment and prevention of exacerbations of chronic diseases: of cardiovascular system, respiratory system, digestive system, pathologies of the urinary tract, etc., and also as a rehabilitation therapy after past diseases.

The purpose of the study is to systematize and generalize the data of world literature concerning the general health-improving and tonic properties study of plants growing in the Republic of Buryatia.

Material and methods. In this work used publication materials from the PubMed and е-library databases, search.rsl. The keyword search: restorative, tonic effect, herbal medicines, medicinal plants of Buryatia, biologically active substances, Astragalus membranaceus, Saposhnikovia divaricatа, Scutellaria baicalensis, Sedum roseum, Crataegus sangunea. The survey comprises the data of foreign and national articles, published on the topic during last 20 years.

Conclusions. All the plants listed in this review are used for thousands of years in the Tibetan medicine. Based on the analysis of the presented literature data, their range of medicinal use is much wider, then the applications in modern clinical practice. The information reported in this review may be the basis for the development of new herbal medicines, including officinal mixture, with specified pharmacological properties.

Introduction. Butein ‒ 2',4',3,4-tetrahydroxychalcone is found in a number of plants that have a pronounced antimicrobial and antifungal effect: Butea monosperma Lam., hairy string ‒ Bidens pilosa L., tripartite string ‒ Bidens tripartita L., hydrophytum ‒ Hydnophytum formicarum Jack., early thyme ‒ Thymus praecox. Butein is not the dominant representative of the flavonoid class in plants, but it can serve as a marker compound for the analysis of raw materials containing this chalcone.

The purpose of the study is to synthesize and analyze a working standard sample of butein, as well as to develop a method for its quantitative determination in Cosmos bipinnate raw materials using solid-phase extraction.

Material and methods. Butein synthesis was carried out using the Claisen‒Schmidt reaction, the yield was 44,4%. Identification of the obtained butein was carried out using UV, IR and Mass spectroscopy. Quantitative determination of butein in the raw materials of Cosmos doubly persita was carried out by HPLC in combination with solid-phase extraction.

Results. The results obtained confirm that the test sample is butein (2',4',3,4-tetrahydroxychalcone). To determine butein in plant raw materials, a standard sample of this compound should be available, and therefore the substance of this chalcone was synthesized and on its basis, according to the state pharmacopoeia, a working standard sample was obtained (butein content in it is 99,62%). Identification of butein by HPLC was carried out by the retention time, which in the chromatogram of the test solution is 160,70 min, in the chromatogram of the standard solution – 160,07 min ± 2%. The butein content in the flowers of the cosmos bipinnate we studied in terms of dry raw materials is 0,043% ± 2%.

Conclusions. The synthesis of chalcone ‒ butein was carried out by alkaline condensation of resacetophenone with protocatechuic aldehyde. The yield of butein is 5,95 ± 0,0195 g (44,4%). A working standard sample was obtained based on the butein substance, the authenticity and purity of which was confirmed by melting point, as well as using HPLC, mass spectroscopy, UV and IR spectroscopy. The resulting standard working sample was further used to develop a method for the quantitative determination of butein in Cosmos bipinnate flowers using solid-phase extraction. The butein content in terms of dry raw materials is 0,043%±3%.

Introduction. In quality control of pharmaceutical formulations, there are methodological approaches based on the preliminary separation of sample components and approaches without preliminary separation. The use of solid-phase extraction (SPE) allows to separate analytes during sample preparation, with the separation selectivity determining the analysis selectivity as a whole. Currently, sorbents that provide selectivity in interaction with the analyte are of interest.

Aim. Evaluation of the metrological characteristics of benzocaine quantitation by UV-spectrophotometry in certain pharmaceutical formulations using hypromellose sorbent for solid-phase extraction.

Material and methods. The sorbent using a method developed by us was obtained. Benzocaine (FS.2.1.0634, Russia State Pharmacopoeia XV ed.) to form active binding sites in sorbent structure was applied. The sorbent structure is polycyanoacrylate matrix with hypromellose fragments, the surface area is 255.50 m²/g, pore volume is 0.1433 cm³, pore diameter is 5.32 nm. The sorption capacity of hypromellose sorbent for benzocaine was 12.2±0.8 μg/g. The SPE technique is proposed. SPE includes the stages: conditioning, sample addition and step-by-step elution with purified water and hydrochloric acid solution 0.1 mol/L. The spectrophotometer SF-56 was used to measure the absorbance at an analytical wavelength of 286 nm. To evaluate the selectivity (specificity) of sample preparation during chromatographic (SPE) separation of pharmaceutical formulations, absorption spectra of eluates in the wavelength range of 200-400 nm were scanned. The benzocaine identification in the eluate was carried out based on absorption peaks at 220 and 286 nm. The benzocaine calibration curve in the range of 1-20 μg/ml. To determination the metrological characteristics of benzocaine quantitation method, 11 parallel determinations of samples of each pharmaceutical formulation were made.

Results. When realizing the benzocaine quantitation technique for measuring absorbance, it is advisable to use only the first portions of eluate (a solution of hydrochloric acid) in a volume of 5-10 ml were obtained. The relative error of benzocaine average concentration in the pharmaceutical formulations ranged from 1.28 to 1.34% for technique that included the SPE stage, and from 1.86 to 2.02% for technique that did not include it. A comparison of the modifications of the spectrophotometric quantitation of benzocaine in pharmaceutical formulation using hypromellose sorbent and technique without the SPE did not reveal a statistically significant difference in reproducibility. For the modification technique without SPE, an increase of benzocaine concentration and in determination relative error by an average of 48.0% were observed.

Conclusion. The possibility of using a selective hypromellose sorbent for sample preparation for benzocaine quantitation determination in pharmaceutical formulations has been showed. The use of a sorbent for SPE at the sample preparation stage reduces the systematic error of benzocaine spectrophotometry quantitation for the analyzed pharmaceutical formulations by an average of 79.2%.

The purpose of the study is to study the component composition of the essential oil in the aerial part of the endemic Krasnodar specimen Thymus pulchellus S. A. Mey., transplanted to the Tsudakhar experimental base of the Mountain Botanical Garden of the Dagestan Federal Research Center of the Russian Academy of Sciences (CEB, 1000 m above sea level, Dagestan, Russia ) to search for plants with a high yield of essential oil with a maximum content of valuable biologically active components and expand the growing area of this type of thyme.

Material and methods. The aerial part of the Thymus pulchellus introduced at the CEB was collected in the flowering phase. The essential oil was obtained by hydrodistillation (Clevenger method) for 2 hours from dry raw materials prepared by conventional methods. Component qualitative and quantitative analysis of the isolated essential oil samples was carried out by chromatography-mass spectrometry on a Shimadzu GCMSQP2010plus on a Supelco SLB TM-5 ms column (30 m × 0.25 mm × 0.25 μm) in split mode.

Results. The EF yield was 0.26% in terms of air-dry raw materials. Using chromatography-mass spectrometry, 56 components were isolated from EF samples, of which 97.67% of compounds were identified.

Conclusions. For the first time, data are presented on the study of the composition of the essential oil from the aerial part of the introduced Dagestan sample of Thymus pulcellus. The resulting essential oil consists mainly of the sesquiterpene fraction (84.12%). Compared to other species, the essential oil of the studied pretty thyme showed a completely different set of main components, among which the main ones are limonene, γ-cadinene, α-muurolol, epicubenol, which allows us to assert the isolation of this species. The results obtained allow us to characterize the essential oil of Thymus pulchellus as a source of valuable components, and the plant itself as a promising medicinal raw material.