Anthocyanin profile and effect at glaucoma Vaccinium arctostaphylos and Vaccinium myrtillus fruits

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Introduction. We may use the sources of anthocyanins for prevention of glaucoma in the medicine practice. Pharmacopoeia source of anthocyanins is fruits of bilberry. Related species such as Vaccinium arctostaphyllos may be additional source of anthocyanins along with Vaccinium myrtillus.

Aim – the comparative investigation of anthocyanin profile of Vaccinium arctostaphylos and V. myrtillus fruits and its effects at the progress of glaucoma.

Material and methods. We prepared two types of extracts: without and with addition of hydrochloric acid. We got anthocyanins profile by high-performance liquid chromatography with UV-detector. Glaucoma model was prepared by injection of polystyrene microballons in anterior chamber of rats with subsequent measurement of activity of succinate dehydrogenase and concentration of thiobarbituric acid reactive products (TBARS) in the rat’s brain tissues.

Results. Water-alcohol extracts with addition of hydrochloric acid from bilberry fruits contain three anthocyanins: delphinidin, cyanidin, mixture peonidin and malvidin. Cyanidin is the major components in the both types of fruits. Water-alcohol extracts without hydrochloric acid content nine substances: delphinidin 3-galactoside, delphinidin 3-glucoside, cyanidin 3-galactoside in mixture with delphinidin 3-arabinoside, cyanidin 3-glucoside, petunidin 3-glucoside, peonidin 3-glucoside, malvidin 3-glucoside. Malvidin 3-glucoside and delphinidin 3-glucoside are the main glycosides in the V. arctostaphylos fruits, cyaniding 3-glucoside and glycosides of delphinidin – in the V. myrtillus fruits. Water-alcohol extracts with addition of hydrochloric acid had more effective as neuroprotective agent by increasing concentration of succinate dehydrogenase and decreasing amount of TBARS in the brain.

Conclusion. Extracts from bilberry fruits have neuroprotective effect by presence of total amount of anthocyanins and may be used for prevention of glaucoma.

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Kh. Ibaeva

Volgograd State Medical University Ministry of Health of Russian Federation

编辑信件的主要联系方式.
Email: ibaeva.hadizhat@mail.ru
ORCID iD: 0009-0002-7900-4559
SPIN 代码: 6678-9776

Post-graduate Student, Department of Pharmacognosy, Botany and Technology of Phytopreparations; Pyatigorsk Medical-Pharmaceutical Institute 

俄罗斯联邦, Kalinina Avenue, 11, Pyatigorsk,357532

A. Shamilov

Volgograd State Medical University Ministry of Health of Russian Federation

Email: shamilovxii@yandex.ru
ORCID iD: 0000-0002-6730-9518
SPIN 代码: 5834-3249

Dr.Sc. (Pharm.), Associate Professor, Professor of Department of Pharmacognosy, Botany and Technology of Phytopreparations; Pyatigorsk Medical-Pharmaceutical Institute 

俄罗斯联邦, Kalinina Avenue, 11, Pyatigorsk,357532

V. Tashlitsky

M.V. Lomonosov Moscow State University

Email: tashlitsky@belozersky.msu.ru
ORCID iD: 0000-0003-4100-2419
SPIN 代码: 2590-8903

Ph.D. (Chem.), Senior Research Scientist, Department of Chemistry of Natural Compounds, Chemical Faculty

俄罗斯联邦, Leninskie Gory, GSP-1, building 40, Moscow, 119991

D. Pozdnyakov

Volgograd State Medical University Ministry of Health of Russian Federation

Email: pozdniackow.dmitry@yandex.ru
ORCID iD: 0000-0003-0889-7855
SPIN 代码: 6764-0279

Ph.D. (Pharm.), Head of Department of Pharmacology with course of Clinical Pharmacology; Pyatigorsk Medical-Pharmaceutical Institute 

俄罗斯联邦, Kalinina Avenue, 11, Pyatigorsk,357532

参考

  1. Zaa C.A., Marcelo Á.J., An Z. et al. Anthocyanins: Molecular Aspects on Their Neuroprotective Activity. Biomolecules. 2023; 13(11): 1598. doi: 10.3390/biom13111598.
  2. Tribble J.R., Hui F., Quintero H. et al. Neuroprotection in glaucoma: Mechanisms beyond intraocular pressure lowering. Mol Aspects Med. 2023; 92: 101193. doi: 10.1016/j.mam.2023.101193.
  3. Panchal S.K., John O.D., Mathai M.L. et al. Anthocyanins in Chronic Diseases: The Power of Purple. Nutrients. 2022; 14(10): 2161. doi: 10.3390/nu14102161.
  4. Sim R.H., Sirasanagandla S.R., Das S. et al. Treatment of Glaucoma with Natural Products and Their Mechanism of Action: An Update. Nutrients. 2022; 14(3): 534. doi: 10.3390/nu14030534.
  5. Фидарова А.Ч., Бериев О.Г. Распространение, биологические особенности и экологическое значение черники кавказской (Vaccinium arctostaphylos L.) в Республике Северная Осетия-Алания. Вестник МАНЭБ. 2008; 14(3): 74–76. [Fidarova A.Ch., Beriev O.G. Ditribution, biological features and ecological importance of Vaccinium arctostaphylos L. of Republic of North Ossetia-Alania. Vestnik MANEB. 2008; 14(3):74-76. (In Russ.)].
  6. Ayaz F.A., Hayirlioglu-Ayaz S., Gruz J. et al. Separation, characterization, and quantitation of phenolic acids in a little-known blueberry (Vaccinium arctostaphylos L.) fruit by HPLC-MS. J Agric Food Chem. 2005; 53(21): 8116–22. doi: 10.1021/jf058057y.
  7. Lätti A.K., Kainulainen P.S., Hayirlioglu-Ayaz S. et al. Characterization of anthocyanins in caucasian blueberries (Vaccinium arctostaphylos L.) native to Turkey. J Agric Food Chem. 2009; 57(12): 5244–5249. doi: 10.1021/jf9005627.
  8. Jooyandeh H., Noshad M., Khamirian R.A. Modeling of ultrasound-assisted extraction, characterization and in vitro pharmacological potential of polysaccharides from Vaccinium arctostaphylos L. Int J Biol Macromol. 2018; 107(Pt A): 938–948. doi: 10.1016/j.ijbiomac.2017.09.077.
  9. Kianbakht S., Abasi B., Dabaghian F.H. Anti-hyperglycemic effect of Vaccinium arctostaphylos in type 2 diabetic patients: a randomized controlled trial. Forsch Komplementmed. 2013; 20(1): 17–22. doi: 10.1159/000346607.
  10. Kianbakht S., Hashem-Dabaghian F. Antihypertensive efficacy and safety of Vaccinium arctostaphylos berry extract in overweight/obese hypertensive patients: A randomized, double-blind and placebo-controlled clinical trial. Complement Ther Med. 2019; 44: 296–300. doi: 10.1016/j.ctim.2019.05.010.
  11. Cásedas G., González-Burgos E., Smith C. et al. Regulation of redox status in neuronal SH-SY5Y cells by blueberry (Vaccinium myrtillus L.) juice, cranberry (Vaccinium macrocarpon A.) juice and cyanidin. Food Chem Toxicol. 2018; 118: 572–580. doi: 10.1016/j.fct.2018.05.066.
  12. Руководство по методам контроля качества и безопасности биологически активных добавок к пище. М.: Федеральный центр госсанэпиднадзора Минздрава России. 2004; 240 с. [Method Guide of quality control and safety of biological active food supplements. M.: federal Centre of gossanepidnadzor of Ministry of of Health. 2004; 240 p.]
  13. Patko E., Szabo E., Vaczy A. et al. Protective Effects of Pituitary Adenylate-Cyclase-Activating Polypeptide on Retinal Vasculature and Molecular Responses in a Rat Model of Moderate Glaucoma. Int J Mol Sci. 2023; 24(17): 13256. doi: 10.3390/ijms241713256.
  14. Nickavar B., Amin Gh. Anthocyanins from Vaccinium arctostaphylos berries. Pharmaceutical Biology. 2004; 42(4-5): 289–291. doi: 10.1080/13880200490511819.
  15. Shamilov A.A., Olennikov D.N., Pozdnyakov D.I. et al. Caucasian Blueberry: Comparative Study of Phenolic Compounds and Neuroprotective and Antioxidant Potential of Vaccinium myrtillus and Vaccinium arctostaphylos Leaves. Life (Basel). 2022; 12(12): 2079. doi: 10.3390/life12122079.

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2. Fig.1. Chromatograms of the extract from the fruits of Vaccinium arctostaphyllos (а) and Vaccinium myrtillus (б) (solvent – 70% ethyl alcohol with a content of concentrated hydrochloric acid 1%)

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3. Fig. 2. Chromatograms of the extract from the fruits of Vaccinium arctostaphyllos (а) and Vaccinium myrtillus (б) (solvent – 70% ethyl alcohol)

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4. Fig. 3. Chromatogram of the extract from frozen fruits Vaccinium myrtillus (solvent – 70% ethyl alcohol)

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5. Fig. 4. Succinate dehydrogenase activity in the brain tissue of animals in different groups: ЧК – animals receiving the extract from Vaccinium arctostaphyllos fruits; ЧО – animals receiving the extract from Vaccinium myrtillus fruits; НК – negative control group; intact – intact animals; * – significant relative to the НК group of rats (Newman–Keuls test, p<0.05); # – significant relative to intact rats (Newman–Keuls test, p<0.05)

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6. Fig. 5. Content of TBARS in the brain of animals of different groups: ЧК – animals receiving extract from Vaccinium arctostaphyllos fruits; ЧО – animals receiving extract from Vaccinium myrtillus fruits; НК – negative control group; intact – intact animals; * – significantly relative to intact rats (Newman–Keuls test, p<0.05)

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