Investigation of phenolic compounds of the jerusalem artichoke herbs (Helianthus tuberosum L.)


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Abstract

Relevance. Jerusalem artichoke (Helianthus tuberosum L.), is a valuable food crop grown in many countries of the world and has multiple uses in the food industry and animal husbandry. The main raw material of Jerusalem artichoke is tubers, when harvesting the grass, as a rule, is utilized. The herb has a significant biomass and contains phenolic compounds. The aim of the study was to analyze the phenolic complex of Jerusalem artichoke herb and assess the prospects for its use as a medicinal plant material. Material and Methods. As a result of the studies carried out by HPLC-UV-MS/MS, 18 compounds related to hydroxycinnamic acids and flavonoids were identified. The dominant substances are chlorogenic, isochlorogenic A and isochlorogenic C acids. Results. Using the method of direct spectrophotometry, a method has been developed for the quantitative determination of the sum of phenolic compounds in terms of chlorogenic acid. The content of these compounds ranges from 4.88 ± 0.22 % to 7.47 ± 0.35 %, the accumulation is carried out mainly in the leaves. Conclusion. The highest content of chlorogenic acid is also observed in Jerusalem artichoke leaves and is 2.65 ± 0.08 %. Thus, Jerusalem artichoke herb harvested at the end of the growing season is of interest for further research and creation on pharmaceutical substances. At the same time, this will make it possible to purposefully process the secondary raw materials formed during the cultivation of tubers and ensure the comprehensive use of Jerusalem artichoke.

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About the authors

O. L Saybel

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Author for correspondence.
Email: info@eco-vector.com

Ph.D. (Pharm.)

Russian Federation, Moscow, Russia

References

  1. Зеленков В.Н. Топинамбур: агробиологический портрет и перспективы инновационного применения. Монография. М.: РГАУ-МСХА, 2012. 161 с.
  2. Государственный реестр селекционных достижений, допущенных к использованию. Т.1. «Сорта растений» (официальное издание). М.: ФГБНУ «Росинформагротех», 2021. 719 с
  3. Xiao Yong Ma, Li Hua Zhang, Hong Bo Shao et al. Jerusalem artichoke (Helianthus tuberosus L.) a medicinal salt-resistant plant has high adaptability and multiple-use values Journal of medicinal plantresearch. 2011; 5(8): Р. 1272-1279. Published Online https://www.researchgate.net/pub-lication/228464875_Jerusalem_artichoke_Helianthus_tube-rosus_a_medicinal_salt-resistant_plant_has_high_adaptabi-lity_and_multipleuse_values
  4. Ярошевич М.И., Вечер Н.Н. Топинамбур (Helianthus tuberosus L.) - перспективная культура многоцелевого использования. Труды БГУ. 2010; 4(2). Режим доступа: http://www.bio.bsu.by/proceedings/articles/2009-4-2-198-208.pdf.
  5. Stimbirys A., Bartkiene E., Siugzdaite Ju., Augeniene D., Vidmantiene D., Juodeikiene G., Maruska A., Stankevicius M., Cizeikiene D. Safety and quality parameters of ready-to-cook minced pork meat products supplemented with Helian-thus tuberosus L. tubers fermented by BLIS producing lactic acid bacteria. J. Food Sci. Technol. 2015; 52(7): 4306-4314.
  6. Зеленков В.Н. Средство лечебной косметики, содержащее экстракты топинамбура. Патент РФ № 2138247. 02.12.1997 г.
  7. Реестр изобретений Российской Федерации [Электронный ресурс]. Режим доступа: https://www1.fips.ru/regis-ters-web/action?acName=clickRegister®Name=RUPAT (дата обращения 06.01.2022 г.).
  8. Song Y., Wi S.G., Kim H.M., Bae H.J. Cellulosic bioethanol production from Jerusalem artichoke (Helianthus tuberosus L.) using hydrogen peroxide-acetic acid (HPAC) pretreatment. Bioresour echnol. 2016 Aug; 214: 30-36. doi: 10.1016/j.biortech.2016.04.065.
  9. Gunnarssona I.B., Svenssonb S.-E., Johanssonc E., Kara-kasheva D., Angelidakia I. Potential of Jerusalem artichoke (Helianthus tuberosus L.) as a biorefinery crop. Industrial Crops and Products. 2014; 56: 231240. doi: 10.1016/j.indcrop.2014.03.010.
  10. Белоусова А.Л., Саенко С.А., Зяблицева Н.С. Фотохимическое исследование травы топинамбура. Материалы регион. конф. по фармации, фармакологи и подготовке кадров. Пятигорск: ПятГФА, 2001; 12-13.
  11. Fujia Chen, Xiaohua Long, Zhaopu Liu, Hongbo Shao, Ling Liu. Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry. Scientific World Journal. 2014; 2014: 568043. Published online 2014 Aug 5. doi: 10.1155/2014/568043.
  12. Carazzone C., Mascherpa D., Gazzani G., Papetti A. Identification of phenolic constituents in red chicory salads (Cichori-um intybus) by high-performance liquid chromatography with diode array detection and electrospray ionisation tandem mass spectrometry. Food Chem. 2013 Jun 1; 138(2-3): 10621071. doi: 10.1016/j.foodchem.2012.11.060.
  13. Clifford M., Zheng W., Kuhnert N. Profiling the chlorogenic acids of aster by HPLC-MSn. Phytochemical analysis. 2006; 17(6): 384-393. doi: 10.1002/pca.935.
  14. Jaiswal R., et al. Profiling and characterization by LC-MS n of the chlorogenic acids and hydroxycinnamoylshikimate esters in mate (Ilex paraguariensis). Journal of Agricultural and Food Chemistry. 2010; 58(9): 5471-5484. doi: 10.1021/jf904537z.
  15. Jaiswal R., Kiprotich J., Kuhnert N. Determination of the hy-droxycinnamate profile of 12 members of the Asteraceae family. Phytochemistry. 2011; 72(8): 781-790. doi: 10.1016/j.phytochem.2011.02.027.
  16. Clifford M. N., Marks S., Knight S., Kuhnert N. Characterization by LC-MS n of four new classes of p-coumaric acid-containing diacyl chlorogenic acids in green coffee beans. Journal of Agricultural and Food Chemistry. 2006; 54(12): 4095-4101. doi: 10.1021/jf060536p.

Supplementary files

Supplementary Files
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1. JATS XML
2. Fig. 1. HPLC-UV chromatogram of water-alcohol extraction from Jerusalem artichoke grass (330 nm): 1 – chlorogenic acid; 2 - quercetin–glucuronide; 3 - isochlorogenic A acid; 4 – luteolin/kaempferol-glucuronide; 5 – isochlorogenic C acid

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3. Fig. 2. UV absorption spectrum of water-alcohol extraction from Jerusalem artichoke grass (1) and chlorogenic acid (2)

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