Expression of inhibitor of cyclindependent kinases р16ink4a in patients with endometrial hyperplasia and secondary infertility associated with external genital endometriosis and uterine myoma

Introduction. The endometrial hyperplasia is a current problem in connection with the high frequency in reproductiveaged patients with infertility associated with external genital endometriosis and uterine myoma. The aim ofthe study was the comparative evaluation of sex steroids expression, inflammatory markers and an inhibitor of cyclindependent kinases p16ink4a in the endometrial hyperplasia. Methods. The study included 54patients with endometrial hyperplasia. 1st group: 30patients with secondary infertility and external genital endometriosis degree I-II, 2nd group: 24patients with secondary infertility and uterine myoma (intramural and subserous form, the diameter of the nodes does not exceed of 3.0 cm). The expression of ER and PgR receptors, inflammatory markers (CD8+, CD20+, CD4+, SD138+) and inhibitor of cyclindependent kinase p16ink4a was studied by the immunohistochemical and immunofluorescent methods. Results. Patients with endometrial hyperplasia showed the same distribution of ER and PR expression in both endometrial histogenetic structures, a high incidence of chronic endometritis, and positive expression of the inhibitor of cyclindependent kinases p16ink4a in the endometrial glands and stroma. Conclusion. The high frequency of chronic endometritis and positive expression of the inhibitor of cyclindependent kinases p16ink4a in the glands and the stroma of the endometrium indicates a violation of the cell cycle of endometrial cells against the background of a chronic inflammatory process and activation of proliferative processes.

endometrial hyperplasia, external genital endometriosis, uterine myoma, estrogen, progesterone, inhibitor of cyclindependent kinases p16