DETERMINATION OF FcγRIIIb (CD16) EXPRESSION ON THE SURFACE OF BLOOD NEUTROPHILS IN ANTI-PLAGUE VACCINATED PEOPLE


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Aim. Comparative evaluation of FcγRIIIb (CD16) expression level on the surface of blood neutrophils in vaccinated and unvaccinated people before and after the interaction of cells in vitro with the plague microbe allergen (pestin). Materials and methods. Blood samples of 25 healthy donors not vaccinated against the plague were examined, as well as 40 volunteers who were vaccinated once and several times with the plague vaccine alive, before and during different periods (1, 6 months) after the vaccination. The expression of CD16 on the surface of neutrophil granulocytes was determined by flow cytometry before and after incubation of blood samples for 4 hours with pestin. Results. After vaccination in all subjects there was registered a significant increase in the expression level of the CD16 molecule on the surface of blood neutrophils. After in vitro contact with pestin, the expression level of the FcγRIIIb molecule (CD16) decreased on the surface of neutrophils only in blood samples of people vaccinated against the plague. Conclusion. The change in the neutrophil phenotype from CD16bright to CD16dim, revealed by in vitro interaction with blood pestin grafted against the plague of people, mimics the response of these cells in anaphylactic shock mediating with specific IgG, and apparently represent the result of the functioning of the FcγRIIIb (CD16) as a trigger for netosis.

Full Text

Restricted Access

About the authors

A. L Kravtsov

Russian Research Anti-Plague Institute «Microbe»

Email: rusrapi@microbe.ru
Saratov, Russian Federation

S. A Bugorkova

Russian Research Anti-Plague Institute «Microbe»

Email: rusrapi@microbe.ru
Saratov, Russian Federation

S. N Klyueva

Russian Research Anti-Plague Institute «Microbe»

Email: rusrapi@microbe.ru
Saratov, Russian Federation

V. A Kozhevnikov

Russian Research Anti-Plague Institute «Microbe»

Email: rusrapi@microbe.ru
Saratov, Russian Federation

O. M Kudryavtseva

Russian Research Anti-Plague Institute «Microbe»

Email: rusrapi@microbe.ru
Saratov, Russian Federation

References

  1. Нестерова И.В., Колесникова Н.В., Чудилова Г.А., Ломтатидзе Л.В., Ковалева С.В., Евглевский А.А. Нейтрофильные гранулоциты: новый взгляд на «старых игроков» на иммунологическом поле. Иммунология. 2015; 36 (4): 257-65.
  2. Eisele N., Lee-Lewis H., Besch-Williford C., Brown C.R., Anderson D.M. Chemokine receptor CXCR2 mediates bacterial clearance rather than neutrophil recruitment in a murine model of pneumonic plague. Am. J. Pathol. 2011; 178 (3): 1190-200. https://doi.org/10.1016/j.ajpath.2010.11.067.
  3. МсDonald B., Urrutia R., Yipp B.G., Jenne C.N., Kubes P. Intravascular neutrophil extracellular traps capture bacteria from bloodstream during sepsis. Cell Host Microbe. 2012; 12 (3): 324-33. https://doi. org/10.1016/j.chom.2012.06.011.
  4. Aleman O.R., Mora N., Cortes-Vieyra R., Uribe-Querol E., Rosales C. Differential use of human Fcy-receptors for inducing neutrophil extracellular traps formation. J. Immunology Research. 2016; 17 pages, ID 2908034, http:// dx.doi.org./10.1155/2016/2908034.
  5. Williams T.E., Nagavajan S., Selvaraj P, Zhu C. Concurrent and independent binding of Fcy receptors IIa and IIIb to surface-bound IgG. Biophysical J. 2000; 79 (4): 1867-75.
  6. Elghetany M.T. Surface antigen changes during normal neutrophilic development: a critical review. Blood Cell. Mol. Dis. 2002; 28: 260-74.
  7. Нестерова И.В., Чудилова Г.А., Ломтатидзе Л.В., Ковалева С.В., Колесникова Н.В., Авдеева М.Г., Русинова Е.В. Дифференцированность вариантов субпопуляций трансформированного фенотипа CD16+CD11b+ нейтрофильных гранулоцитов при острой вирусной и острой бактериальной инфекциях. Иммунология. 2016; 37 (4): 199-204. https://doi. org/10.18821/0206-4952-2016-37-4-199-204.
  8. Dudte S.C., Hinnebusch B.J., Shannon J.G. Characterization of Yersinia pestis interactions with human neutrophils in vitro. Front Cell Infect. Microbiology. 2017; 7: 358. https://doi.org/10.3389/fcimb.2017.00358.
  9. Landoni V.I., Chiarella P, Martire-Greco D., Schierloh P, van-Rooijen N., Rearte B., Palermo M.S., Isturiz M.A., Fernandez G.C. Tolerance to lipopolysaccharide promotes an enhanced neutrophil extracellular traps formation leading to a more efficient bacterial clearance in mice. Clinical and Experimental Immunology. 2012; 168: 153-63. https://doi. org/10.1111/j.1365-2249.2012.04560.x.
  10. Фрадкин В.А. Диагностика аллергии реакциями нейтрофилов крови. М.: Медицина, 1985; 176.
  11. Коробкова Е.И. Кожная аллергическая реакция как показатель иммунитета при чуме. Журнал микробиологии, эпидемиологии и иммунобиологии. 1955; 4: 40-7.
  12. Бугоркова С.А., Щуковская Т.Н., Микшис Н.И., Клюева С.Н., Кудрявцева О.М., Кравцов А.Л., Гончарова А.Ю., Кожевников В.А., Санджиев Д.Н., Конушева С.В., Савченко С.П., Щербакова С.А., Кутырев В.В. Комплексное иммунологическое исследование вакцинированных живой чумной вакциной лиц, проживающих на территории Прикаспийского песчаного очага чумы в Республике Калмыкия. Эпидемиология и вакцинопрофилактика. 2018; 17 (3): 38-50. https://doi. org/10.31631/2073-3046-2018-17-3-38-50.
  13. Tosi M.F., Berger M. Functional differences between the 40 kDa and 50 to 70 kDa IgG Fc-receptors on human neutrophils revealed by elastase treatment and antireceptor antibodes. J. Immunology. 1988; 141 (6): 2097-103.
  14. Pham C.T. Neutrophil serine proteases fine-tune the inflammatory response. Int. J. Biochem. Cell Biol. 2008; 40 (6-7): 1317-33.
  15. Papayannopoulos V., Metzier K.D., Hakkim A., Zychlinsky A. Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps. J. Cell Biol. 2010; 191: 677-91.
  16. Khodoun M.V., Strait R., Armstrong L., Yanase N., Finkelman F.D. Identification of markers that distinguish IgE- from IgG-mediated anaphylaxis. PNAS. 2011; 108 (30): 12413-8. https:/doi.org/10.1073/pnas. 1105695108.
  17. Branzk N., Lubojemska A. Hardison S.E., Wang Q., Guttierrez M.G., Broun G.D., Papayannopoulos V. Neutrophil sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nat. Immunol. 2014; 15 (11): 1017-25. https://doi.org/10.1038/ni.2987.
  18. Monteseirin J., Bonilla I., Camacho M.J., Chason P, Vega A., Chaparro A., Conde J., Sobrino F. Specific allergens enhance elastase release in stimulated neutrophils from asthmatic patients. Int. Arch. Allergy Immunology. 2003; 131 (3): 174-81.
  19. Кравцов А.Л., Шмелькова Т.П., Щуковская Т.Н. Влияние противочумной вакцинации на функциональную активность клеток врожденного иммунитета человека. Проблемы особо опасных инфекций. 2011; 107: 77-80.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2020 Russkiy Vrach Publishing House

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies