Comparative influence of hyaluronic acid on proliferation and apoptosis of skin cells in middle-aged women


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Abstract

Introduction. Hyaluronic acid in combination with other biology active molecules and ions is an efficient drug for the function restoration and normalizing an external appearance of the skin during aging. However, the comparative investigation of hyaluronic acid biological activity in combination with other additives for bio arming with molecular methods using wasn’t applying yet. The aim of the study is to investigate the influence of hyaluronic acid in combination with various ions and other additives on skin cell proliferation and apoptosis in middle-aged women. Methods. Skin biopsies from middle-aged women were used after bio arming with applying hyaluronic acid drugs in combination with other biological active additives. The investigation of Ki67 and p53 proteins expression in skin cells was done by immunofluorescence confocal methods with quantitative analysis of the data by morphometric method. Results. Injections of hyaluronic acid without additives increased Ki67 proliferative protein expression in skin cells of middle-aged women by 2.9 times and didn’t’ changed on p53 proapoptosis protein expression. Injections of hyaluronic acid with magnesium and silicium ions increased Ki67 expression in skin cells of middle-aged women by 5.3 and 4.3 times and p53 expression - by 3.1 and 3.3 times. Conclusion. The most effective way of skin functional activity increasing is injections of hyaluronic acid for bio arming procedure in middle-aged women. Injections of hyaluronic acid with magnesium and silicon ions stimulate proliferation and apoptosis of skin cells and can be applying for young women’s skin revitalization.

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About the authors

V. N Khabarov

Hyaluronic acid Research and Development Center

Email: miayy@yandex.ru
Komsomolsky pr., 38/16-84, Moscow, 119146, Russian Federation

I. M Kvetnoy

Saint Petersburg Institute of Bioregulation and Gerontology; St. Petersburg University; Saint Petersburg Research Institute of Phthisiopulmonology

Email: miayy@yandex.ru
Dynamo pr., 3, Saint Petersburg, 197110, Russian Federation; Universitetskaya emb., 7/9, Saint Petersburg, 199034, Russian Federation; Ligovsky prospekT., 2-4, Saint Petersburg, 191036, Russian Federation

N. S Linkova

Saint Petersburg Institute of Bioregulation and Gerontology; Academy of postgraduate education of the Federal Medical Biological Agency of Russia; Belgorod State University

Email: miayy@yandex.ru
Dynamo pr., 3, Saint Petersburg, 197110, Russian Federation; Volokolamskoe Shosse, 91, Moscow, 125371, Russian Federation; Pobedy str., 85, Belgorod, 308015, Russian Federation

M. A Paltsev

Lomonosov Moscow State University Center of Immunology and Molecular Biomedicine

Email: miayy@yandex.ru
Faculty of Biology Leninskie Gory, d 1, page 12, Moscow, 119234, Russian Federation

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