Mechano-modified form of orotic acid in the correction of hepatopathy caused by hyperhomocysteinemia

Introduction. The therapeutic effect of orotic acid (OA) is limited by the low absorption of the drug from the gACTrointestinal tract, which can be overcome when the drug is processed by mechanical activation. The aim of the study is a comparative experimental research of the action of mechano-modified orotic acid in the conditions of hepatopathy caused by hyperhomocysteinemia. Methods. Preparations of orotic acid were administered in the initial form (IOA) and after mechanical activation (MOA) to white outbred rats with hyperhomocysteinemia. The blood was examined for liver enzymes, bilirubin, cytological blood analysis was performed. Histological research included calculation of density of cell location, the area of the functional parenchyma, the nuclear apparatus, the microvascular bed (100 μm2). Results. The use of MOA shows the more pronounced reduction of АЛТ - by 1.5 times; AP - 1.2 times; total bilirubin - 1.2 times (p<0.05), WBC - by 36.06%, PLT - by 11.4%. The administration of MOA increases the level of red blood cells and hemoglobin. Histological research shows MOA to provide a more pronounced positive effect on the total area of the functional parenchyma (higher than in IOA by 23.2%), the number of nuclei and the total area accounted for by the nuclear apparatus of hepatocytes (higher than in IOA by 1.44 times higher). Conclusion. The mechano-modified preparation of orotic acid has many advantages in comparison with the original analog, which allows us to offer its clinical testing as a therapeutic agent for various types of toxic liver damage.

mechano-modified form of orotic acid, homocysteine-induced hepatopathy, liver enzymes, hepatocytes