Innate and adaptive immunity during infection and immunization with inactivated tick-borne encephalitis vaccine
- Authors: Morozova O.V.1,2, Isaeva E.I.1
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Affiliations:
- Federal Research Clinical Center of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russia
- National Research Center of Epidemiology and Microbiology of N.F. Gamaleya of the Russian Ministry of Health
- Issue: Vol 19, No 6 (2021)
- Pages: 43-48
- Section: Articles
- URL: https://journals.eco-vector.com/1728-2918/article/view/113533
- DOI: https://doi.org/10.29296/24999490-2021-06-07
- ID: 113533
Cite item
Abstract
Introduction. Viral infections induce cellular and humoral immunity that provide virus elimination. Extracellular presentation of antigens of formaldehyde-inactivated vaccines causes mainly humoral immune response with risk of persistent infection. The aim of the study. comparison of dynamics of cytokines and antibodies IgMand IgG during infection and immunization of laboratory mice with inactivated vaccine against the tick-borne encephalitis. Methods: reverse transcription - real time PCR (RT2-PCR); ELISA to detect IgM and IgG antibodies. Results: All studied cytokine mRNA were found in the virus-infected mice whereas IL1fi, IL4 and TNFa RNA were missing after vaccination. Cytokine gene expression began in 1-2 days postinfection with maximal values in 4-8 days and subsequent decline, however, after vaccination cytokine RNA were revealed in 14 days only and were detected for 42 days of observations, probably, due to 3 subsequent injections. Antibodies in infected mice were also registered earlier, in 7-14 days postinfection, whereas after immunization later - in 28-42 days after first injection and antibody titers were higher. Conclusion: During viral infection cytokine profiles were more diversified, cytokine RNA transcription and IgM and IgG secretion took place earlier than for vaccination. Titers of antibodies were higher in immunized mice after 2-3 intramuscular injections compared to infection with long-lasting IgM circulation for 42 days.
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About the authors
Olga Vladimirovna Morozova
Federal Research Clinical Center of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russia; National Research Center of Epidemiology and Microbiology of N.F. Gamaleya of the Russian Ministry of Health
Email: omorozova2010@gmail.com
Leading researcher
Elena Ivanovna Isaeva
Federal Research Clinical Center of Physical-Chemical Medicine of the Federal Medical and Biological Agency of Russia
Email: immunol.lab@mail.ru
Head of the Laboratory of Immunology
References
- Морозова О., Бахвалова В. Вакцины против клещевого энцефалита: прошлое, настоящее и будущее. Lambert Academic Publishing (LAP), Saarbrucken, Germany, 2011. ISBN 978-3-8433-1159-5, 100 c.
- Игнатьев ГМ., Отрашевская Е.В., Воробьева М.С. Активность цитокинов при иммунизации вакциной против клещевого энцефалита в эксперименте. Вопросы вирусологии. 2003; 2: 22-5.
- Погодина В.В., Фролова М.П., Ерман Б.А. Хронический клещевой энцефалит Новосибирск: «Наука» Cибирское отделение, 1986; 232.
- Рекомендации ГИСК им. Тарасевича по контролю активности отечественных вакцин против КЭ. М., 2010.
- Морозова О.В., Бахвалова В.Н., Чичерина Г.С., Потапова О.Ф., Исаева Е.И. Cравнение экспрессии генов цитокинов у мышей, иммунизированных или зараженных вирусом клещевого энцефалита. Сборник научных статей к 80-летию академика РаМН Ф.И. Ершова. М., 2012; 461-5.
- Morozova O.V., Bakhvalova V.N., Potapova O.F., Grishechkin A.E., Isaeva E.I., Aldarov K.V., Klinov D.V., Vorovich M.F. Evaluation of immune response and protective effect of four vaccines against the tick-borne encephalitis virus. Vaccine. 2014; 32 (25): 3101-6. https://doi.org/10.1016/j.vaccine.2014.02.046.
- Marth E., Kleinhappl B. Albumin is a necessary stabilizer of TBE-vaccine to avoid fever in children after vaccination. Vaccine. 2002; 20: 532-7. https://doi.org/10.1016/s0264-410x(01)00329-2.
- Морозова О.В., Ткачев С.Е., Чудинов А.П. «Детекция РНК в вакцине «Энцевир» против вируса клещевого энцефалита», Вопросы вирусологии. 2006; 51 (3): 42-6.
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