Effect of immobilization of glycoproteins of the baker’s yeast cell wall on their immunomodulatory activity

The aim of the study was to study the effect of chemical immobilization of yeast cell wall glycoproteins on their ability to activate cytokine production by human peripheral blood cells. Methods. A comparative analysis of the immunomodulatory activity of yeast cell wall glycoproteins in a free and immobilized state was carried out. The dynamics of the process of accumulation of proinflammatory cytokines (IL6, IL8, and TNFa) in the extracellular medium after contact of human blood cells with glycoproteins was studied. The source of glycoproteins was the cell lysate of Saccharomyces cerevisiae. The glycoproteins were immobilized on a polyacrylamide gel in a copolymerization reaction. The study of the effectiveness of immobilized and free glycoproteins was carried out in in vitro experiments on the whole blood of practically healthy donors. The effectiveness was evaluated by the increase in the concentration of cytokines in the blood plasma after contact with glycoproteins compared to the baseline level, as well as by comparing the effect of polyacrylamide gel without a ligand and simply incubating blood cells with saline solution. Results. It was found that immobilized glycoproteins, as well as free ones, in contact with human peripheral blood lead to the activation of the synthesis of pro-inflammatory cytokines (IL6, IL8, and TNFa) by blood cells. The study revealed the peculiarities of cytokine synthesis depending on the form of contact of blood cells with the glycoprotein. It is shown that the rate of IL8 and TNFa synthesis is higher after blood contact with immobilized glycoproteins than with free-form glycoproteins.

cytokines, glycoproteins, yeast, interleukin-6, immunomodulators