Molecular markers of endometrial carcinoma and associated clinicopathological features

Ekaterina Igorevna Koroleva; Королёва Екатерина Игоревна; Aram Ashotovich Musaelyan; Мусаелян Арам Ашотович; Vladimir Dmitrievich Nazarov; Назаров Владимир Дмитриевич; Sergej Vladimirovich Lapin; Лапин Сергей Владимирович; Sergey Leonidovich Vorobev; Воробьев Сергей Леонидович; Olga Lvovna Sharova; Шарова Ольга Львовна; Evgeniya Sergeevna Kozorezova; Козорезова Евгения Сергеевна; Inna Mihaylovna Korablina; Кораблина Инна Михайловна; Vladimir Leonidovich Emanuel; Эмануэль Владимир Леонидович; Sergey Vladimirovich Orlov; Орлов Сергей Владимирович

doi:10.29296/24999490-2022-03-06

Molecular markers of endometrial carcinoma and associated clinicopathological features

Authors: Koroleva E.I.¹, Musaelyan A.A.¹^,2, Nazarov V.D.¹, Lapin S.V.¹, Vorobev S.L.³, Sharova O.L.³, Kozorezova E.S.³, Korablina I.M.³, Emanuel V.L.¹, Orlov S.V.¹^,2
Affiliations:
1. P. Pavlov First Saint-Petersburg State Medical University
2. Federal State Budget Research Institution "Research Institute of Medical Primatology"
3. National Center for Clinical Morphological Diagnostics
Issue: Vol 20, No 3 (2022)
Pages: 34-40
Section: Articles
URL: https://journals.eco-vector.com/1728-2918/article/view/113753
DOI: https://doi.org/10.29296/24999490-2022-03-06
ID: 113753

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Abstract

Introduction. In 2013 The Cancer Genome Atlas research group divided the molecular aberrations in endometrial cancer into four subtypes: POLE mutations, microsatellite instability, copy-number low and copy-number high. These subtypes have different prognosis and could probably mean different therapeutical options. The aim of the study. To estimate the prevalence of different molecular aberrations and to find out associated clinicopathological features. Methods. The study included 45 samples of endometrial carcinoma. Pathological structure and molecular features of each sample were evaluated. Immunohistochemical testing was used to find out the level of p53 expression. Genetic testing was carried out by PCR for MSI testing and by sequencing POLE exon 9 and exon 13 for point mitations. Results. Nor POLE mutations, nor abnormal p53 expression level were found in studied samples. The prevalence was 22% for MSI tumors. No significant difference in clinicopathological features was found between MSI and MSS tumors except for mitotic index that was higher in MSI tumors. Similarly, MSI tumors were less differentiated but the difference was not statistically significant. Conclusion. POLE mutations and abnormal p53 level are rare in low-grade endometrial carcinomas. Nevertheless, MSI tumors are common in this group and further research of their prognostic significance is important.

Keywords

POLE, MSI, p53, endometrial cancer, molecular classification, POLE, MSI, p53

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