Induction of hyperglycemia and accompanying biochemical and genotoxic changes in mice of different strains by tyloxapol
- Authors: Kachalov K.S.1, Solomina A.S.1, Rodina A.V.1, Kulakova A.V.1, Zhanataev A.K.1, Durnev A.D.1
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Affiliations:
- Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies
- Issue: Vol 22, No 3 (2024)
- Pages: 45-52
- Section: Reviews
- URL: https://journals.eco-vector.com/1728-2918/article/view/633669
- DOI: https://doi.org/10.29296/24999490-2024-03-07
- ID: 633669
Cite item
Abstract
Introduction. Hyperglycemia inducers, the «classic» diabetogens streptozotocin and alloxan have a pronounced effect and are therefore limited suitable for modeling mild hyperglycemia in an experiment. Tyloxapol (Triton WR1339) was chosen as an alternative agent for modeling a moderate increase in blood glucose concentration in experimental animals based on literature data.
The purpose of the study. The aim of the work was to investigate the possibility of tyloxapol to induce hyperglycemia and concomitant biochemical and genotoxic changes in mice of the common C57BL/6, CBA/lac and ICR lines.
Methods. Hyperglycemia was modeled by single or multiple administration of tiloxapol at a dose of 400 mg/kg intraperitoneally. 24 hours after the last injection of tyloxapol, glucose and lipid levels were determined in mouse blood samples, DNA damage was assessed in organs by the DNA comet method, and chromosomal aberrations were recorded in bone marrow cells.
Results. It was found that tyloxapol at a dose of 400 mg/kg with different modes of administration to mice of different lines similarly causes moderate hyperglycemia and a significant increase in triglycerides, total cholesterol and low-density lipoproteins, as well as an increase in DNA damage, which is considered as a trigger for many complications in diabetes.
Conclusion. The data obtained in mice are consistent with the literature data on the hyperglycemic activity of tyloxapol, previously established in rats, and demonstrate characteristic biochemical and genotoxic effects in mice of different lines, with less severity in animals of the ICR line. In general, the data obtained indicate the possibility of using tiloxapol to model experimental diabetes in mice.
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About the authors
Kirill S. Kachalov
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies
Author for correspondence.
Email: kkachalov@mail.ru
ORCID iD: 0000-0003-1340-5034
graduate student, junior researcher Laboratory of Genetic and Reproductive Toxicology of the Drug Toxicology Department
Russian Federation, Baltiyskaya street, 8, Moscow, 125315Anna S. Solomina
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies
Email: annsolomina@yandex.ru
ORCID iD: 0000-0001-7048-4993
leading researcher of Laboratory of Genetic and Reproductive Toxicology of the Drug Toxicology Department, Candidate of Biological Sciences
Russian Federation, Baltiyskaya street, 8, Moscow, 125315Anastasia V. Rodina
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies
Email: an.vl.rodina@gmail.com
ORCID iD: 0009-0002-4238-050X
junior researcher of Laboratory of Genetic and Reproductive Toxicology of the Department of the Drug Toxicology Department
Russian Federation, Baltiyskaya street, 8, Moscow, 125315Alla V. Kulakova
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies
Email: allakulak@mail.ru
ORCID iD: 0000-0002-6959-2150
senior researcher of Laboratory of Genetic and Reproductive Toxicology of the Department of the Drug Toxicology Department, Candidate of Biological Sciences
Russian Federation, Baltiyskaya street, 8, Moscow, 125315Alii K. Zhanataev
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies
Email: zhanataev_ak@academpharm.ru
ORCID iD: 0000-0002-7673-8672
head of the laboratory of Laboratory of Genetic and Reproductive Toxicology of the Department of the Drug Toxicology Department, Candidate of Biological Sciences
Russian Federation, Baltiyskaya street, 8, Moscow, 125315Andrey D. Durnev
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies
Email: durnev@academpharm.ru
ORCID iD: 0000-0003-0218-8580
head of Drug Toxicology Department, Dr. Sci (Med.), professor, corresponding member RAS
Russian Federation, Baltiyskaya street, 8, Moscow, 125315References
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