Neurofilament (Nf-L) in the rat cerebral ischemia experiment

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Introduction. Additionally to electrophysiological and clinical methods the neurofilament light chains (Nf-L) concentration in blood plasma is considered as a marker for predicting functional outcomes.

The aim of the study. Assessment of neurofilament light chains (Nf-L) level in plasma in 21 days after experimental stroke.

Methods. A randomized controlled trial was conducted on mature male rats of the SHR lines (n = 20) and WKY (n = 20) weighing 250 ± 50 g. Cerebral ischemia was performed by monofilament occlusion of the left middle cerebral artery under anesthesia (Zoletil +Xylazine) at a dose of 0,88 ml/kg.

Results. On Day 1 post- MCA occlusion, WKY animals had a Garcia score 6 and SHR rats had a score 5 out of 18. WKY testing data on days 3, 5, and 7 revealed decreased muscle strength (MCAo: 2.0 ± 0.1 N; Sham: 3.0 ± 0.2N; p < 0.05). SHR testing data on on days 3, 5 revealed a decrease (MCAo: 1.9 ± 0.2N; Sham: 3.0 ± 0.2N, p < 0.05). At the same postoperative time, the Nf-L concentration in SHR animals was 2.7-fold higher compared to controls and different from WKY animals.

Conclusion. The change in Nf-L concentration in rats after SHR stroke is more pronounced than in WKY rats. A change in the concentration of Nf-L in blood plasma indicates the predominant course of destructive processes in the nervous tissue. Stroke in SHR rats is accompanied by a more pronounced and prolonged decrease in muscle strength and impaired coordination.

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作者简介

Maria Kolpakova

Pavlov Institute of Physiology, Russian Academy of Sciences

编辑信件的主要联系方式.
Email: kolpakovame@infran.ru
ORCID iD: 0000-0003-3013-5582

Senior Researcher, Candidate of medical sciences, Associate Professor

俄罗斯联邦, Makarova Emb., 6, St-Petersburg, 199034

Anastasia Yakovleva

First Pavlov State Medical University

Email: biomed.1spbgmu@yandex.ru
ORCID iD: 0000-0002-1889-5928

Researcher, Candidate of biological sciences

俄罗斯联邦, L. Tolstoy str., 6–8, St-Petersburg, 197022

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2. Fig. 1. Garcia score in WKY (1, ) and SHR (1, б) rats

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3. Fig. 2. Muscle strength test for right forelimb in WKY rats

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4. Fig. 3. Muscle strength test for right forelimb in SHR rats

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5. Fig. 4. Changes in plasma Nf-L concentration

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