Effect of the S-nitrosoglutathione nitric oxide donor on the relative amount of P-selectin in vitro
- Authors: Korotkova N.V.1, Kalinin R.E.1, Suchkov I.A.1, Abalenikhina Y.V.1, Mzhavanadze N.D.1, Nikiforov A.A.1, Fomina D.I.1
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Affiliations:
- Ryazan State Medical University
- Issue: Vol 23, No 3 (2025)
- Pages: 101-107
- Section: Original research
- URL: https://journals.eco-vector.com/1728-2918/article/view/688986
- DOI: https://doi.org/10.29296/24999490-2025-03-13
- ID: 688986
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Abstract
Introduction. Studying the effect of the nitric oxide (II) donor, S-nitrosoglutathione, on the relative amount of selectin P in HUVEC cell lysates in vitro may contribute to fundamental understanding of the effect of the nitric oxide system on the adhesive function of the endothelium.
Objective: To evaluate the effect of nitric oxide (II) donor S-nitrosoglutathione (GSNO) on the activity of mitochondrial endotheliocyte oxidoreductases, the relative amount of selectin P and nitric oxide metabolites in HUVEC cell lysates, and the absolute amount of sP-selectin (soluble) in a conditioned medium.
Material and methods. The mitochondrial dehydrogenase activity of HUVEC cells was evaluated using an MTT test. The relative amount of P-selectin in endotheliocyte lysates was determined by the Western blot method, the absolute amount of the soluble selectin fragment (sP) in the cellular supernatant was determined by the sandwich ELISA method, the level of persistent nitric oxide (II) metabolites was determined by the method based on the reduction of nitrates to nitrites in the presence of vanadium chloride and subsequent spectrophotometric detection using Griss reagent.
Results. The study found that upon joint incubation of the primary HUVEC cell line with nitrosoglutathione solutions in various concentrations, the development of nitrosative stress is noted, as evidenced by an increase in its markers – persistent nitric oxide (II) metabolites in cell lysates. Under conditions of nitrosative stress, there is a decrease in the activity of mitochondrial endotheliocyte oxidoreductases, which may be due to the negative effects of excess nitric oxide or its toxic products. With a three-hour exposure of nitrosoglutathione to endotheliocytes at a low concentration of 10 mkM, there is a statistically significant increase in the relative amount of P-selectin; at high concentrations of 100-500 mkM, there is a statistically significant decrease in the relative amount of P-selectin.
Conclusion. The results obtained in this study indicate the effect of nitric oxide (II) on the functional response of the endothelium by quantifying the cell adhesion molecule P-selectin.
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About the authors
Natalya V. Korotkova
Ryazan State Medical University
Author for correspondence.
Email: fnv8@yandex.ru
ORCID iD: 0000-0001-7974-2450
SPIN-code: 3651-3813
Associate Professor, аsociate Professor of the Department of Biological Chemistry, Senior researcher at the Central Research laboratory
Russian Federation, Vysokovoltnaya str., 9, Ryazan, 390026Roman E. Kalinin
Ryazan State Medical University
Email: r.kalinin@rzgmu.ru
ORCID iD: 0000-0002-0817-9573
SPIN-code: 5009-2318
Doctor of Medical Sciences, Professor in cardiovascular surgery, Head of the Department of cardiovascular, endovascular surgery, and diagnostic radiology
Russian Federation, Vysokovoltnaya str., 9, Ryazan, 390026Igor A. Suchkov
Ryazan State Medical University
Email: i.suchkov@rzgmu.ru
ORCID iD: 0000-0002-1292-5452
SPIN-code: 6473-8662
Doctor of Medical Sciences, Professor in cardiovascular surgery, Professor of the Department of cardiovascular, endovascular surgery, and diagnostic radiology
Russian Federation, Vysokovoltnaya str., 9, Ryazan, 390026Yulia V. Abalenikhina
Ryazan State Medical University
Email: abalenihina88@mail.ru
ORCID iD: 0000-0003-0427-0967
Doctor of Medical Sciences, PhD in biochemical sciences, Professor of the Department of Biological Chemistry, Leading researcher at the Central Research laboratory
Russian Federation, Vysokovoltnaya str., 9, Ryazan, 390026Nina D. Mzhavanadze
Ryazan State Medical University
Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0001-5437-1112
SPIN-code: 7757-8854
Doctor of Medical Sciences, PhD in cardiovascular surgery, Professor the department of cardiovascular, endovascular surgery and diagnostic radiology, Leading researcher at the central research laboratory
Russian Federation, Vysokovoltnaya str., 9, Ryazan, 390026Alexander A. Nikiforov
Ryazan State Medical University
Email: a.nikiforov@rzgmu.ru
ORCID iD: 0000-0001-9742-4528
SPIN-code: 8366-5282
PhD in pharmaceutical sciences, Associate professor of the department of pharmacology, Head of the Central Research laboratory
Russian Federation, Vysokovoltnaya str., 9, Ryazan, 390026Darya I. Fomina
Ryazan State Medical University
Email: fdarya18@yandex.ru
ORCID iD: 0009-0008-5532-6303
5th year student at the Faculty of Medicine
Russian Federation, Vysokovoltnaya str., 9, Ryazan, 390026References
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