MOLECULAR MECHANISMS OF RETARDATION OF SKIN AGING UNDER INFLUENCE 0F HUMAN PLACENTA HYDROLYSATE


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Introduction. Application of placenta hydrolysate in cosmetology opens a new page in anti-aging therapy. Numerous studies have confirmed the antioxidant, anti-inflammatory, anti-pigmental properties of placenta hydrolysate preparations, as well as activation of trophic function and optimization of cellular metabolism. The difference between preparation Melsmon and its analogs is the maximum safety of the drug since it does not include hormones, growth factors, enzymes, it is completely free from toxic substances. Purpose of the study. To evaluate the possibility of using the preparation Malsmon as a regulatory and geroprotective agent for the prevention of skin aging of human fibroblasts during their senescence in vitro Methods. The expression of proteins was compared by immunocytochemistry in cell cultures of human skin fibroblasts at 7th and 14th passages with the administration of preparation Melsmon and in the control group. Results. When analyzing the data of the relative area of expression of the studied markers in the «young culture» of human fibroblasts, an increase in the expression indices of Ki-67, calreticulin, sirtuin-6 andsyntaxin under the influence of the Melsmon preparation was found after the 3rd passage. Whereas in the «old culture» on the 14th passage, when placental hydrolysate (Melsmon) was added, activation of the expression of ki-67, sirtuins 1 and 6, to the same level as in the young (control) culture was observed. Conclusion. The Melsmon preparation has pronounced geroprotective properties with respect to human skin cells, which gives arguments for its further development as a means of stimulating regenerative processes in the skin and preventing its aging.

全文:

受限制的访问

作者简介

I. Kvetnoy

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St.Petersburg University

Saint Petersburg, Russian Federation

A. Drobintseva

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University

Email: anna.drobintseva@gmail.com
Saint Petersburg, Russian Federation

T. Kleimenova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University

Saint Petersburg, Russian Federation

V. Polyakova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St.Petersburg University

Saint Petersburg, Russian Federation

K. Turkadze

Sechenov University

Moscow, Russian Federation

参考

  1. Banerjee K.K., Bishayee A., Chatterjee M. Anti-inflammatory effect of human placental extract: a biochemical mechanistic approach. European Review for Medical and Pharmacological Sciences. 1992; 14 (6): 361-6.
  2. Watanabe S., Togashi S.I., Takahashi N. et al. L-tryptophan as an antioxidant in human placenta extract. J. of Nutritional Science and Vitaminology 2002; 48 (1): 36-9.
  3. Tamura T. A study on the anti-fatigue of the placental extract (Melsmon) and its hormone-like application. Pharmacology and Treatment. 1978; 6 (10): 31-6.
  4. Nordlund J.J., Halder R. An analysis of published and other available data. Dermatologica. 1990; 181 (1): 1-4.
  5. Kim J.H., Choi S.Y., Kim S.O. et al. Effect of local placental extract injection on pigmentary disorders. Abstracts of the Korean J. of Dermatology 2005; 43 (2): 175.
  6. Kim H.J., Lee J.W., Kim Y.I., Lee M.H. The effect of placental extract on the expression of tyrosinase, TRP-1 and TRP-2 in SK30 melanoma cells. Korean J. of Dermatology 2003; 41 (12): 1612-8.
  7. http://melsmon.ru/melsmon/istoriya-sozdaniya/
  8. Коваленко И.И., Аталян А.В. Опыт применения гидролизата плаценты у женщин с климактерическим синдромом в перименопаузе. Гинекология. 2016; 18 (5): 20-5.
  9. Измайлова Т.Д. Персонализированные протоколы метаболической коррекции как основа anti-age программ. Инъекционные методы в косметологии. 2016; 1: 24-37.
  10. Измайлова Т.Д. Персонализированная ПРП-терапия. Алгоритм подготовки пациента. Инъекционные методы в косметологии. 2016; 2: 19-23.
  11. Родионов А.Н., Смирнова И.О., Корнишева В.Г. Дерматология для косметологов. Наука и Техника. 2014; 767.
  12. Ноздрин В.И., Горелова М.В., Белоусова Т.А. Возрастные изменения эпидермиса кожи волосистой части головы у мужчин. Морфология. 2011; 139 (1): 74-81.
  13. Ольховский И.А., Горбенко А.С., Столяр М.А. и соавт. Определение мутации в гене кальретикулина у пациентов с подозрением на хронические миелопролиферативные неоплазии. Гематол. и трансфузиол. 2014; 59 (3): 12-5.
  14. Daugas E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. J. Exp. Med. 2000; 192 (4): 571-80.
  15. Sharma S. et al. Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively Nucleic Acids Res. 2013; 41 (19): 9062-76.
  16. Baur J.A. Resveratrol, sirtuins, and the promise of a DR mimetic. Mech Ageing Dev. 2010; 131: 261-9.
  17. McCord R.A., Michishita E., Hong T, Berber E., Boxer L.D., Kusumoto R., Guan S., Shi X., Gozani O., Burlingame A.L., Bohr V.A., Chua K.F. SIRT6 stabilizes DNA-dependent protein kinase at chromatin for DNA double-strand break repair. Aging (Albany NY). 2009; 1 (1): 109-21.
  18. Mao Z., Hine C., Tian X., Van Meter M., Au M., Vaidya A., Seluanov A., Gorbunova V. SIRT6 promotes DNA repair under stress by activating PARP1. Science. 2011; 332 (6036): 1443-6.
  19. Michishita E. et al. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins. Mol. Biol. Cell. 2005; 16 (10): 4623-35.
  20. McBurney M.W., Yang X., Jardine K. et al. The mammalian SIR2alpha protein has a role in embryogenesis and gametogenesis. Mol. Cell Biol. 2003; 23 (1): 38-54.
  21. Yang S., Liu T., Li S., Zhang X. et al. Comparative proteomic analysis of brains of naturally aging mice. Neuroscience. 2008; 154 (3): 1107-20.
  22. Jung J.J., Inamdar S.M., Tiwari A., Choudhury A. Regulation of intracellular membrane trafficking and cell dynamics by syntaxin-6. Biosci. Rep. 2012; 32: 383-91.

补充文件

附件文件
动作
1. JATS XML
下载
##common.cookie##