The possibilities of anomalous viscosity time dependence method for determination of cell radiosensitivity

The initial state of an organism and its initial reactivity are the main prognostic criteria for the individual radiosensitivity evaluation. Particulars of the DNA structure and chromatin conformation determine in many respects the cell radiosensitivity. The method of the anomalous viscosity time dependence (AVTD) allows to research the chromatin conformational state, as well as to evaluate its reactivity based on the response to hyperthermia (heat shock). The DNA damage degree after irradiation under the test dose is a general criterion for the cell radiosensitivity evaluation. The goal of research. The work is aimed to analyse the radiation-induced DNA damage dependence on the initial chromatin conformational state and reactivity. Material and methods. The radiobiological studies were performed using the cells of white outbred male rats in vitro. Using the AVTD method, with respect to each animal, it was determined the initial chromatin conformational state and reactivity. After gamma radiation exposure of 60Со at the dose of 3,0 Gy it was determined the DNA damage degree using DNA-comet assay. Results. In a group of animals in general, the irradiation led to a considerable increase of the DNA damage degree relative to the spontaneous level (р0,001). It was found the meaningful positive correlative dependence (r=0,37; p0,05) of the individual radiationinduced DNA damage degree on the initial chromatin reactivity to heat shock.

blood cells, chromatin, anomalous viscosity time dependence method, heat shock, DNA damage, comet assay, radiosensitivity