Tamparing of cytokine content in serum and platelet soluble preparations, produced in different ways

Introduction. Human platelets maintain big reparative and regenerative potential. The biochemical content of platelet preparations seriously depends on methodic features. The aim of the study. To study cytokine content in different types of platelet soluble preparations and serum, harvested from donors-volunteers. Methode. Conserved donors' blood was performed into 5 types of preparations: platelet-rich plasma (PRP), platelet-rich plasma with a high concentration of leucocytes (L-PRP), platelet-poor plasma (PPP), passively sedimented plasma (PasSP), platelet-rich suspension in non-plasmatic solution (PRS). We also harvested non-conserved blood for serum extraction. Platelet lysates were produced by cell cryodestruction at -40/-80С, followed by defrosting at +2/+4°С during 12 hours and 3000g centrifugation. Results. Lysates of PRP, L-PRP, and PRS were shown to have an intensively higher level of growth factors, comparing to serum. Curiously, in PPP concentration of IL 1alfa, VEGF and TGFa were higher, than in PRP. The presence of leukocytes in PRP enhanced the level of PDGF and pro-inflammatory cytokines IL 6, IL 8 in lysates, whereas the level of VEGF in L-PRP was significantly decayed. In PasSP-lysates concentration of VEGF and FGF was similar to serum values and was rather lower, than in PRP and PPP. Platelet re-washing from plasma led to 2,3-4,9 times rise of PDGF, EGF, VEGF, FGF concentrations in PRS-lysates. We found a strong correlation between PDGF and EGF, VEGF, and TNFa in lysates, PDGF and EGF level also correlated with the concentration of platelets with granules in samples before cryodestruction. Common platelet concentration in PRP did not correlate with any one of the cytokines in the lysate. Conclusion. Growth factors could be both elevated in PRP and PPP. Platelet rewashing significantly enhanced the volume of growth factors in the lysate.

platelets, granules, cytokines, growth factors, concentration