Shagen metabolism dynamics in tissues of rats with experimental diabetes on the background of chronic emotional stress


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Introduction. Developing of chronic emotional stress reduces adaptive capabilities of people ill with diabetes mellitus causing the condition of decompensation with significant elevation of counterinsular hormones production and further progressive metabolic disturbances in the body tissues. Wherein, studying metabolism of the main structural component of the connective tissue, - collagen, is of interest since qualitative and quantitative changes of the given biopolymer metabolism are considered to be one of the chief link of diabetes mellitus pathogenesis. The aim of the study. To study the indices of collagen metabolism in tissues of rats with experimental diabetes developing under prolonged emotional stress. Methods. Total collagen content, neutral salt-soluble collagen and free hydroxyproline were determined in the myocardium, the liver and diaphysis of the right femur in diabetic rats under prolonged influence of stress factors applying the colorimetric method. Listed above indices were studied in dynamics of the experiment on the 10th, 20th and 30th day. Results. Stress load in alloxan-induced animals with diabetes caused changings in collagen metabolism in the organs studied. Significant accumulation of total collagen was noted in the liver, mainly due to intensification processes of synthesis. In the myocardium it was noted speed increase of collagen synthesis on the 10th and 20th days of the experiment, activation of decay processes occurred in the last decade. In the bone tissue the content of total collagen in animals with combined impact did not differ from the indice in diabetic rats. Conclusion. Daily stress impacts on animals with alloxan diabetes stimulated the accumulation of total collagen in the liver, caused phase changings of collagen metabolism indices in the myocardium, but they did not aggravate the decrease of the total collagen content in the bone tissue, observed in experimental diabetes.

全文:

受限制的访问

作者简介

Natalia Savinova

Izhevsk State Medical Academy

Email: biochem2017@mail.ru
Assistant Professor

Olga Danilova

Izhevsk State Medical Academy

Email: danilova-stlab@yandex.ru
Assistant Professor

Svetlana Perevedentseva

Izhevsk State Medical Academy

Email: perevedenceva69@mail.ru
Assistant Professor

Saniya Trofimova

Izhevsk State Medical Academy

Email: saniyatr@yandex.ru
Assistant Professor

Natalia Naumova

Izhevsk State Medical Academy

Email: naumova_ng@mail.ru
Head of the Department of Biochemistry.

参考

  1. Hilliard M. E., Yi-Frazier J.P., Hessler D., Butler A. M., Anderson B.J., Jaser S. Stress and A1c Among People with Diabetes Across the Lifespan. Curr Diab Rep. 2016; 16 (8): 67. doi: 10.1007/s11892-016-0761-3
  2. Kitfbchi A. E., Umpierrez G. E., Miles J. M., Fisher J.N. Hyperglycemic Crises in Adult Patients with Diabetes. Diabetes Care. 2009; 32 (7): 1335-43. DOI: https://doi.org/10.2337/dc09-9032
  3. Пальчикова Н.А., Кузнецова Н.В., Кузминова О.И., Селятицкая В.П. Гормонально-биохимические особенности аллоксановой и стрептозотоциновой моделей экспериментального диабета. Бюллетень СО РАМН. 2013; 33 (6): 18-24.
  4. Тигранян РА. Метаболические аспекты проблемы стресса в космическом полете. Проблемы космической биологии. 1985; 52: 223.
  5. Резников А.Г. Методы определения гормонов. Киев: Наукова думка, 1980, 399.
  6. Шараев П.Н., Сахабутдинова Е.П., Лекомцева О.И., Кошикова С.В. Определение свободного и пептидосвязанного гидроксипролина в сыворотке крови. Клин. лаб. диагностика. 2009; 1: 7-9.
  7. Прошина Л.Я., Приваленко М.Н. Исследование фракционного состава коллагена в ткани печени. Вопросы мед химии. 1982; 1: 115-9.
  8. Спасов А.А., Соловьева О.А., Кузнецова В.А. Гликирование белков при сахарном диабете и возможности его фармакологической коррекции. Химикофармацевтический журнал. 2017; 51 (6): 3-7. DOI: https://doi.org/10.30906/0023-1134-2017-51-6-3-7
  9. Чехлов В.В., Абрамова А.Ю., Перцов С.С. Корреляция показателей ноцицепции и иммунных параметров у крыс с разной поведенческой активностью в условиях хронического стресса. Российский журнал боли. 2019; 17 (51): 26-7.
  10. Zhang Y., Yao X. Role of c-Jun N-terminal kinase and p38/activation protein-1 in interleukin-1β-mediated type I collagen synthesis in rat hepatic stellate cells. APMIS Journal of Pathology, Microbiology and Immunology. 2012; 120 (2): 101-7. DOI: https://doi.org/10.1111/j.1600-0463.2011.02816.x
  11. Marty M., Hiriart J.B., Vergniol J., Foucher J., De Ledinghen V, Gin H., Rigalleau V. Steatosis, Glycation and Liver Fibrosis in Patients with Diabetes. J. Diabetes Metab. 2015; 6: 12. doi: 10.4172/2155-6156.1000633
  12. Wei B., Zhu Z., Xiang M., Song L., Guo W., Lin H., Li G., Zeng R. Corticosterone suppresses IL-1β-induced mPGE2 expression through regulation of the 11β-HSD1 bioactivity of synovial fibroblasts in vitro. Experimental and Therapeutic medicine. 2017; 13 (5): 2161-8. doi: 10.3892/etm.2017.4238
  13. Jin L., Zhang J., Deng Z., Liu J., Han W., Chen G., Si Y., Ye P. Mesenchymal stem cells ameliorate myocardial fibrosis in diabetic cardiomyopathy via the secretion of prostaglandin E2. Stem Cell Res Ther 2020; 11: 122. doi: 10.1186/s13287-020-01633-7
  14. Nyman J.S., Even J.L., Jo C.H., Herbert E.G., Murry M.R., Cockrell G.E., Wahl E.C., Bunn R.C., Lumpkin C.K.Jr., Fowlkes J.L., Thrailkill K.M. Increasing duration of type 1 diabetes perturbs the strengthstructure relationship and increases brittleness of bone. Bone. 2011; 48 (4): 733-40. DOI: 10.1016/j. bone.2010.12.016
  15. Kalaitzoglou E., Popescu I., Bunn R.C., Fowlkes J.L., Thrailkill K.M. Effects of type 1 diabetes on osteoblasts, osteocytes and osteoclasts. Curr Osteoporos Rep. 2016; 14 (6): 310-9. doi: 10.1007/s11914-016-0329-9

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Russkiy Vrach Publishing House, 2021
##common.cookie##