Influence of κ- and λ-carrageenans from Chondrus armatus on colon cancer cells proliferation RKO and HCT-116

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Introduction. To this date the search for new substances with antitumor activity is an urgent task of pharmacology and medicine. Carrageenans are sulfated galactans of red algae, they are promising substances for the basis of future drugs, as well as for t he creation of preventive antitumor agents. In this work, we investigated the antitumor effect of carrageenans, different in structure and molecular weight, isolated from the algae Chondrus armatus on the cell lines by colon carcinomas and colorectal carcinoma. Aim of the study: comparative study of the antitumor effect of κ- and λ-carrageenans from the red alga Chondrus armatus and their oligosaccharides on tumor cell lines RKO and HCT-116. Material and methods. Carrageenans κ- and λ- and their oligosaccharide derivatives (κ- and λ-) were derived, characterized by structure (IR spectrometry) and molecular weight (Choromatography), and tested on RKO colon carcinoma and HCT-116 colorectal carcinoma cell lines. The antitumor effect was assessed using the MTT assay-cell proliferation assay and using fluorescent cell dyes of vivo Hoechst staining and propidium iodide to stain living and dead cells. Results and discussion. According to these data, carrageenans and their oligosaccharides exhibit a pronounced inhibitory effect on the proliferation of intestinal carcinoma tumor cells. Carrageenans κ- and λ- had a significant antitumor effect at a maximum concentration of 400 μg / ml during incubation for 24 and 48 hours in the investigated cell lines of intestinal carcinomas RKO and HCT-116. Conclusion. It was shown that oligosaccharides κ- and λ-carrageenans, isolated from the red alga Chondrus armatus, exhibit a pronounced inhibitory effect on the proliferation of human intestinal cancer tumor cells, without increasing the percentage of dead cells. Consequently, these carrageenans exhibit a pronounced antitumor effect blocking cell growth

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作者简介

Vladlena Tiasto

Far Eastern Federal University

编辑信件的主要联系方式.
Email: tiasto_va@dvfu.ru
research assistant scientist in laboratory of Pharmacology and Biotesting, School of biomedicine

参考

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2. Fig. 1. IR spectra: κ-carrageenan, κo-carrageenan, λ-carrageenan and λo-carrageenan isolated from the red alga Chondrus armatus. The numbers in the graphs indicate the absorption bands

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3. Fig. 2. Chromotograms of κ-, κо, λ-, λо-carrageenans. The graphs show the peaks and molecular weights of the substances

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4. Fig. 3. Proliferation of RKO and HCT-116 cell lines after treatment with κ, κо-, λ, λо-carrageenans for 24 and 48 h at concentrations (50, 100, 400 μg/ml) according to the MTT test results. Results are expressed as mean and standard error of the mean (* – significant difference from negative control p <0,05)

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5. Fig. 4. Viability of RKO and HCT-116 cell lines after treatment with κ, κо-, λ, λо-carrageenans for 24 and 48 h at concentrations of 50, 100 and 400 μg / ml according to the results of the viability test. The results are indicated as the number of living and dead cells

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