LEVEL OF DNA FRAGMENTATION IN HUMAN SPERM CELLS IN VARICOCELE AND PROSTATITIS


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Varicocele and prostatitis are the most common andrological diseases, which may be accompanied by a decrease in the production of sperm cells, the deterioration of their quality and increased risk of infertility. This work was aimed to the evaluation of sperm DNA fragmentation index (DFI) and main indices of sperm fertility (concentration, motility and morphology), and the relationship between these parameters in the men of active reproductive age suffering from prostatitis or varicocele. Assessment of sperm DNA fragmentation was performed by SCSA (sperm chromatin structure assay) using flow cytometry; sperm parameters were evaluated according to WHO recommendations. It was shown that men with prostatitis (n = 9) and varicocele (n = 22) had significantly higher DFI compared with men in the control group (n = 22). Negative influence of these diseases on the concentration and the percentage of motile sperm cells in the ejaculate was revealed. These data suggest that the deterioration in the quality of semen in varicocele and prostatitis may be caused not only by pathospermia, but also, at least partially, by violation of the integrity of the sperm DNA. Evaluation of sperm DNA fragmentation can be recommended for use in laboratory diagnostics for prediction of fertility in infertile men.

Толық мәтін

Рұқсат жабық

Авторлар туралы

L. Osadchuk

FSBIS “Institute of Cytology and Genetics”, Siberian Branch of the Russian Academy of Sciences

Email: losadch@bionet.nsc.ru
MD, Professor, Leading Researcher, Endocrinological Genetics sector Novosibirsk

A. Erkovich

Novosibirsk State Medical University

Department of Urology Novosibirsk

D. Tataru

LLC “Krasnoyarsk Center for Reproductive Medicine”

Krasnoyarsk

E. Markova

LLC “Krasnoyarsk Center for Reproductive Medicine”

Krasnoyarsk

A. Svetlakov

LLC “Krasnoyarsk Center for Reproductive Medicine”

Krasnoyarsk

Әдебиет тізімі

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