Tissue-engineered substitution urethroplasty based on decellularized vascular matrix and autologous cells of the buccal mucosa: the first experience


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Urethral strictures and anomalies remain a challenging urological problem. Reconstructive plastic surgery has been shown to be the most effective way to treat them. There are two main types of urethroplasty: anastomosis (anastomotic urethroplasty) and expansion of the urethral lumen using of flaps and grafts (substitution urethroplasty). Currently the ideal material for substitution urethroplasty does not exist. Tissue engineering of the urethra seems to be one of the most promising approaches to address this problem. Various tissues-engineering techniques were proposed for substitution urethroplasty. In this study, tissue-engineering design was based on the decellularized cadaveric arterial wall. The study results demonstrated the feasibility of creating stable tissue-engineered structures with autologous cultured epithelial cells of the buccal mucosa and decellularized matrix from human cadaveric arterial wall (DMCAW). There was a complete engraftment of tissue-engineering design based on DMCAW and buccal mucosa cells, used for substitution urethroplasty in a patient with the bulbar urethral stricture. Postoperatively (within 4 months after surgery) no complications and/or adverse events were observed. However, in the late postoperative period (12 months) there was recurrence of urethral stricture in the middle of the tissue-engineering design and the native urethra that warranted another surgery. Tissue-engineering design based on DMCAW and autologous buccal mucosa is safe as a material for substitution urethroplasty. Further research is required to ascertain the effectiveness of the method.

Толық мәтін

Рұқсат жабық

Авторлар туралы

P. Glybochko

Sechenov First Moscow State Medical University

Ju. Aljaev

Research Institute of Uronephrology and Human Reproductive Health

V. Nikolenko

Sechenov First Moscow State Medical University

A. Shehter

Research Institute of Molecular Medicine

A. Vinarov

Research Institute of Uronephrology and Human Reproductive Health

L. Istranov

Research Institute of Molecular Medicine

E. Istranova

Research Institute of Molecular Medicine

R. Abojanc

Research Institute of Molecular Medicine

A. Ljundup

Research Institute of Molecular Medicine

M. Danilevskij

Research Institute of Uronephrology and Human Reproductive Health

A. Guller

Research Institute of Molecular Medicine

P. Elistratov

Research Institute of Molecular Medicine

D. Butnaru

Research Institute of Uronephrology and Human Reproductive Health

D. Kantimerov

Sechenov First Moscow State Medical University

Email: kantimeroff@gmaiI.com

G. Mashin

Sechenov First Moscow State Medical University

A. Titov

Sechenov First Moscow State Medical University

A. Proskura

Sechenov First Moscow State Medical University

K. Kudrichevskaja

Sechenov First Moscow State Medical University

Әдебиет тізімі

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