Phenotypic characterization of Burkholderia mallei mutants defected in the synthesis of extracellular proteases
- 作者: Ageeva NP1, Merinova LK1, Viktorov DV1, Plekhanova NG1, Ageeva NP1, Merinova LK1, Victorov DV1, Plehanova NG1
-
隶属关系:
- 期: 卷 2, 编号 2 (2005)
- 页面: 11-16
- 栏目: Articles
- URL: https://journals.eco-vector.com/1994-9480/article/view/117670
- ID: 117670
如何引用文章
全文:
详细
Strains of B. mallei, defective in production of extracellular enzymes were isolated using transposone mutagenesis method. Mutants were been characterized by activity of extracellular enzimes, virulence and some other properties. The method of electrophoresis in SDS - PAAG and immunoblotting were imployed for analysing of protein profiles. It was revealed that composition and quantity of protein accounted for changes of proteolytic activity.
参考
- Агеева Н.П., Меринова Л.К. // Генетика и биохимия вирулентности возбудителей особо опасн. инф.: матер. Росс. научн. конф. Волгоград, 1992. С. 26-27.
- Беляков В.Д., Ряпис Л.А., Илюхин В.И. Псевдомонады и псевдомонозы. М.: Медицина, 1990. 224 с.
- Илюхин В.И. // Журн. микробиол., эпидемиол., иммунобиол. 1985. № 2. С. 110-112.
- Попов С.Ф., Мельников Б.И., Курилов В.Я. и др. // Взамиодействие возбудителя мелиоидоза с фагоцитами хозяина: сб. научн. работ. Волгоград, 1990. Вып. 4. С. 184-190.
- Bach-Toan-Vinh. // Ann. Inst. Past. 1965. Vol. 109, № 3. P. 460-468.
- Burtnick M., Bolton A., Brett P., et al. // J. Micro-biol. 2001. Vol. 147. P. 111-120.
- De Shazer D., Brett P.J., Burtnick M.W. et al. // J. Bacteriol. 1999. Vol. 181. P. 4661-4664.
- Difco manual. Dehydrated culture media and reagents for microbiology. Тenth edition. // Difco laboratories, Detroit, Michigan 48232, USA, 1155 pp.
- Korbsrisate S., Suwanasai N., Lulaporn A., et al. // J. Clin. Microbiol. 1999. Vol. 37. P. 3742-3745.
- Laemmli U.K. // Nature. 1970. Vol. 227. P. 680-685.
补充文件
![](/img/style/loading.gif)