Phenotypic characterization of Burkholderia mallei mutants defected in the synthesis of extracellular proteases
- 作者: Ageeva NP1, Merinova LK1, Viktorov DV1, Plekhanova NG1, Ageeva NP1, Merinova LK1, Victorov DV1, Plehanova NG1
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- 期: 卷 2, 编号 2 (2005)
- 页面: 11-16
- 栏目: Articles
- URL: https://journals.eco-vector.com/1994-9480/article/view/117670
- ID: 117670
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Strains of B. mallei, defective in production of extracellular enzymes were isolated using transposone mutagenesis method. Mutants were been characterized by activity of extracellular enzimes, virulence and some other properties. The method of electrophoresis in SDS - PAAG and immunoblotting were imployed for analysing of protein profiles. It was revealed that composition and quantity of protein accounted for changes of proteolytic activity.
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