Comparison of distribution of erythropoietin medications isoforms with two-dimensional gel-electrophoresis
- Authors: Shnaider A1
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- Issue: No 2 (2010)
- Pages: 50-53
- Section: Articles
- URL: https://journals.eco-vector.com/2075-3594/article/view/245864
- ID: 245864
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Abstract
Purpose. Comparison of physicochemical properties of different preparations of recombinant human erythropoietin.
Material and methods. Preparations of recombinant human erythropoietin - Eprex ® and Eralfon® - were compared with each other using two-dimensional gel electrophoresis and polyacrylamide gel electrophoresis over sodium dodecyl sulfate (SDS-PAGE electrophoresis) with Western blot.
Results. The degree of glycosylation of erythropoietin in the drug Eprex ® is exceed the same in the drug Eralfon ®. More basic isoforms of erythropoietin in the preparation Eralfon ® may indicate a short half-life. Eralfon ® contains a significantly greater number of isoforms of erythropoietin as compared with the drug Eprex ®.
Conclusion. Physico-chemical properties of recombinant human erythropoietin are different, that can lead to the change of their efficacy and tolerability.
Material and methods. Preparations of recombinant human erythropoietin - Eprex ® and Eralfon® - were compared with each other using two-dimensional gel electrophoresis and polyacrylamide gel electrophoresis over sodium dodecyl sulfate (SDS-PAGE electrophoresis) with Western blot.
Results. The degree of glycosylation of erythropoietin in the drug Eprex ® is exceed the same in the drug Eralfon ®. More basic isoforms of erythropoietin in the preparation Eralfon ® may indicate a short half-life. Eralfon ® contains a significantly greater number of isoforms of erythropoietin as compared with the drug Eprex ®.
Conclusion. Physico-chemical properties of recombinant human erythropoietin are different, that can lead to the change of their efficacy and tolerability.
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