Mechanisms of platelet-leukocyte aggregates formation in chronic obstructive pyelonephritis


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Abstract

Aim of the study. To ascertain participation of platelets and the role of agonists (epinephrine and ADP) in the formation of platelet-leukocyte aggregates at maintaining the chronic inflammation and the initiation of acute inflammatory response in patients with chronic obstructive pyelonephritis (COPN). Material & Methods. 43 COPN patients (26 in remission and 17 in relapse) were included in the study. Platelets were isolated following centrifugation of citrated peripheral blood. Evaluation of platelet aggregation was performed by Chrono-Log Platelet Aggregometer (USA) using agonist in EC50 concentration. The formation of platelet-leukocyte aggregates (PLA) was simulated in vitro by incubation of platelets stimulated by epinephrine or ADP and intact leukocytes isolated from peripheral blood of COPN patients. Morphometric analysis of the smears was conducted after staining of the cell suspension by the Romanovsky-Giemsa method. Results. The total number of intact platelet-leukocyte aggregates in the peripheral blood in COPN relapse was 29.8% higher than that in remission (p<0.001). After platelet pre-stimulation, their interaction with the intact (non-stimulated) leukocytes was found to increase. a more pronounced effect on platelet-leukocyte aggregate formation in COPN remission was produced by adrenaline and in relapse by ADP. Agonists simulate a certain ratio between platelets and different leukocytes, which reflects the characteristic features of blood cell interaction in the course of chronic and acute inflammation. Conclusion. Gaining insight into the mechanisms regulating the platelet-leukocyte aggregate formation in the course of inflammation offers new opportunities to substantiate COPN recurrence control.

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