GENETIC CHARACTERISTIC OF HIV-1 IN PATIENTS WITH EXPERIENCE OF THERAPY VIROLOGIC FAILURE IN KYRGYZ REPUBLIC IN 2017-2018


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Abstract

Objective. To carry out the molecular-genetic analysis of HIV-1 variants circulated in Kyrgyzstan in 2017-2018 among patients with failure of antiretroviral therapy. Materials and methods. Flasma samples (n = 100) was collected, HIV-1 pol-gene nucleotide sequences were studied, the subtype of the virus in the samples was determined, as well as the structure and level of drug resistance of HIV-1. Results. It was determinate the domination of HIV-1 recombinant variant CRF63 02A1 (in 56% of samples) and sub-subtype A6 ((in 56% of samples) in Kyrgyzstan. At the same time, there were revealed the cases of infection with a rare genetic form of HIV-1, close to CRF94_cpx and CRF95 02B. It was found a high frequency of resistance mutations to drugs included in the main treatment regimens used in the country: to EFV and NVP (in 48 and 50% of samples, respectively) and to FTC and 3TC (in 39% of samples). Conclusion. The high drug resistance frequency to the main ART regimen drugs in patients with high treatment adherence indicates the need for further drug resistance monitoring in the country, including among patients without treatment experience and who are just starting it.

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About the authors

Ilya A. LAPOVOK

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: i_lapovok@mail.ru
Cand. Biol. Sci., Senior Researcher Moscow, Russia

Daria V. SALEEVA

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: dasha_saleeva@inbox.ru
Laboratory Researcher Moscow, Russia

Aleksey E. LOPATUKHIN

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: a.lopatukhin@gmail.com
Researcher Moscow, Russia

Alina A. KIRICHENKO

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: kotova-kirichenko@mail.ru
Researcher Moscow, Russia

Anastasia V. MURZAKOVA

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: murzakova_a.v@mail.ru
Researcher Moscow, Russia

Aibek A. BEKBOLOTOV

Republican AIDS Center of Ministry of Health of Kyrgyz Republic

Email: aibek_0001@mail.ru
Deputy Director Bishkek, Kyrgyzstan

Baarnisa M. ISKANOVA

Republican AIDS Center of Ministry of Health of Kyrgyz Republic

Email: i.barnisa@hotmail.com
Laboratory Doctor Bishkek, Kyrgyzstan

Elmira B. NARMATOVA

Osh AIDS Center of Ministry of Health of Kyrgyz Republic

Email: narmatova@yandex.ru
Chief Physician Osh, Kyrgyzstan

Zhyldyz K. AKMATOVA

Republican AIDS Center of Ministry of Health of Kyrgyz Republic

Email: zhyldyz_akmatova@mail.ru
Epidemiologist Bishkek, Kyrgyzstan

Nazgul A. ASYBALIEVA

Republican AIDS Center of Ministry of Health of Kyrgyz Republic

Email: nazgul-asybaliev@mail.ru
Epidemiologist Bishkek, Kyrgyzstan

Dmitry E. KIREEV

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: dmitkireev@yandex.ru
Cand. Biol. Med., Researcher Moscow, Russia

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