Clonal groups of uropatogenic escherichia coli isolated in the russian federation in 2004–2019

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Resumo

Objective. Identification and characteristics of clonal groups of E. coli isolated from patients with urinary tract infections in the Russian Federation in 2004–2019.

Materials and methods. E. coli strains (n = 303) isolated in 2004–2019 from urine samples of patients with urological diagnoses. The microbiological, molecular genetic and bioinformatic methods for assessing resistance and virulence, as well as molecular genetic methods for identifying phylogenetic groups, O-groups and sequence types were used in the study.

Results. The widespread distribution of uropathogenic strains of the O25-B2-ST131 clonal group in Russia has been shown; in addition, O2-B2-ST141, O89-A-ST744, O4/O6-B2-ST127, B1-ST58, D-ST69, O2/O6-B2-ST73, A-CC10 and O75-B2-CC14clonal groups have been identified.

Conclusion. Assessing the distribution of clonal groups of uropathogenic strains in Russia allows to assess the epidemic situation, predict its development in the future, and also determine the optimal directions of therapy.

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Sobre autores

Pavel Slukin

State Scientific Center for Applied Microbiology and Biotechnology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Autor responsável pela correspondência
Email: xopgi@yandex.ru
ORCID ID: 0000-0002-4976-0145

Researcher, Department of Molecular Microbiology

Rússia, Obolensk, Moscow Region

Nadezhda Fursova

State Scientific Center for Applied Microbiology and Biotechnology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: n-fursova@yandex.ru
ORCID ID: 0000-0001-6053-2621

Cand. Biol. Sci., Leading Researcher, Department of Molecular Microbiology

Rússia, Obolensk, Moscow Region

Eduard Svetoch

State Scientific Center for Applied Microbiology and Biotechnology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: svetoch@obolensk.org
ORCID ID: 0000-0002-3185-1954

DVSc, Professor, Chief Researcher, Department of Molecular Microbiology

Rússia, Obolensk, Moscow Region

Tamara Perepanova

N.A. Lopatkin Scientific Research Institute of Urology and Interventional Radiology – Branch of the National Medical Research Radiological Center, Ministry of Health of Russia

Email: perepanova2003@mail.ru

МD, Professor, Head, Group of Infectious and Inflammatory Diseases and Clinical Pharmacology

Rússia, Moscow

Marina Ershova

Infectious Clinical Hospital № 1

Email: mary.erschowa2013@yandex.ru
ORCID ID: 0000-0003-4691-648X

Head, Microbiological Laboratory

Rússia, Yaroslavl

Elena Poletaeva

Infectious Clinical Hospital № 1

Email: mary.erschowa2013@yandex.ru
ORCID ID: 0000-0002-7074-6989

Bacteriologist

Rússia, Yaroslavl

Aleksandr Kruglov

Moscow Multidisciplinary Clinical Center «Kommunarka» of the Moscow Healthcare Department

Email: kruglov_a_n@mail.ru
ORCID ID: 0000-0001-6849-0008

Cand. Biol. Sci., Senior Researcher, Head, Laboratory of Clinical Microbiology

Rússia, Moscow

Tatyana Priputnevich

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: t_priputnevich@oparina4.ru
ORCID ID: 0000-0002-4126-9730

Professor, Corresponding Member of the Russian Academy of Sciences, МD, Director

Rússia, Moscow

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2. Figure. Representation of Е. coli collection strains (n = 303), resistant to antimicrobial drugs (а), carrying antibiotic resistance genes (b) and pathogenicity factor genes (с) АМР - ampicillin, АМС - amoxicillin/clavulanate, СТХ- cefotaxime, CIP - ciproftoxacin, LVX- levoftoxacin, GEN - gentamicin, AMI - amikacin, FOS - fosfomycin, NIT - nitrofurantoin, СТХ-М - blaстх-м gene, ТЕМ - blaTEM gene, ОХА - blaОХА-48 gene

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