GENETIC TYPING OF BRUCELLA MELITENSIS STRAINS ON THE BASIS OF VARIABILITY ANALYSIS OF INDEL LOCI


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Objective. To perform INDEL typing of Brucella melitensis strains isolated in different geographic regions in 1948 to 2015 for 10 loci (RS05410, RS06765, RS11140, RS12095, RS12365, RS14755, RS16525, RS05465, RS14470, and RS16540). Materials and methods. Molecular genetic typing of 11 B. melitensis strains was carried out by polymerase chain reaction with electrophoretic detection of results. Forty-eight B. melitensis strains present in the GenBank international database were genotyped in silico. Results. Five INDEL genotypes were identif ied among the 59 studied strains. Based on cluster analysis, all genotypes were grouped into 5 clusters. A correlation was established between the INDEL genotype of B. melitensis strains and their belonging to one of the 5 main genetic lines of the microorganism based on whole genome SNP analysis (wgSNP). Conclusion. The findings allow us to consider the developed INDEL typing method as a promising additional tool for defining the origin of individual B. melitensis isolates during epidemiological investigations of brucellosis outbreaks.

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Sobre autores

Dmitry KOVALEV

Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: kovalev_da.stv@list.ru
Cand. Med. Sci., Head, Laboratory of Biochemistry Stavropol, Russia

Irina KUZNETSOVA

Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
Bacteriologist, Laboratory of Biochemistry Stavropol, Russia

Andrew ZHIROV

Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
Researcher, Laboratory of Biochemistry, Antiplague Institute Stavropol, Russia

Natalia SERDUK

Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
Cand. Biol. Sci., Biologist, Laboratory of Collection of Pathogenic Microorganisms Stavropol, Russia

Elena ZHILCHENKO

Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
Cand. Biol. Sci., Head, Laboratory of Collection of Pathogenic Microorganisms Stavropol, Russia

Dmitry PONOMARENKO

Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
Cand. Biol. Sci., Head, Laboratory of Brucellosis Stavropol, Russia

Alexey VODOPYANOV

Rostov-on-Don Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: alexvod@gmail.com
Cand. Med. Sci., Head, Group of Virology Rostov-on-Don, Russia

Sergey VODOPYANOV

Rostov-on-Don Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: serge100v@gmail.com
MD, Head, Laboratory of Microbial Biochemistry Rostov-on-Don, Russia

Aleksandr KULICHENKO

Stavropol Antiplague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
Professor, MD, Corresponding Member of the Russian Academy of Sciences; Director Stavropol, Russia

Bibliografia

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