Experience of using continuous-spectrum pulsed UV radiation of plasma xenon lamps for decontamination of PCR products

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Objective. Evaluation of the efficiency of short PCR amplicons destruction using a continuous spectrum pulsed radiation source.

Materials and methods. Using a pulsed UV source, experiments were conducted on 117 bp DNA amplicons. The efficiency of decontamination was assessed based on the residual amount of amplicons in washes from work surfaces such as plastic, glass, and metal.

Results. High efficiency of pulsed UV radiation in combating DNA contamination was demonstrated, and the optimal irradiation time (10–12 min), sufficient for complete destruction of amplicons on various types of surfaces (plastic, glass, metal) was determined. The data obtained make it possible to reduce the time of room treatment and reduce the negative impact of UV radiation on laboratory equipment and premises.

Conclusion. The feasibility of using pulsed units generating high-intensity continuous spectrum UV radiation flows for the destruction of amplicons on various surfaces was demonstrated.

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Sobre autores

Aleksey Tutelyan

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Autor responsável pela correspondência
Email: bio-tav@yandex.ru
ORCID ID: 0000-0002-2706-6689

Professor, Corresponding Member of the Russian Academy of Sciences, МD, Head, Laboratory of Infections Related to Medical Cares

Rússia, Moscow

Evgeny Cherkashin

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: e.cherkashin@pcr.ms
ORCID ID: 0000-0002-3627-6047

Cand. Chem. Sci., Head, Center for Product Development, Innovation and Development

Rússia, Moscow

Tatiana Zamotaeva

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: zamotaevatat@gmail.com
ORCID ID: 0009-0003-9799-3749

Researcher, Center for Product Development, Innovation and Development, Center for Development, Product Development and Innovation

Rússia, Moscow

Olga Abrosimova

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: kvasova@cmd.su
ORCID ID: 0000-0002-4545-1804

Epidemiologist, Head, Epidemiological Service, Center for Development, Product Development and Innovation

Rússia, Moscow

Aleksey Potapenko

Entreprise de recherche et de production «Melitta»

Email: alexeu1999pt@gmail.com

Researcher

Rússia, Moscow

Sergey Shashkovsky

Entreprise de recherche et de production «Melitta»

Email: melitta916@gmail.com

Cand. Techn. Sci., Chief Designer

Rússia, Moscow

Yakov Goldshtein

Entreprise de recherche et de production «Melitta»

Email: yagmed@gmail.com

General Director

Rússia, Moscow

Vasily Akimkin

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: vgakimkin@yandex.ru
ORCID ID: 0000-0003-4228-9044

Professor, Academician of the Russian Academy of Sciences, MD, Director

Rússia, Moscow

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