EFFECT OF LIPID PEROXIDATION INTENSITY IN BLOOD PLASMA FROM DIFFERENT REGIONS ON THE AREA OF ULCER DEFECT IN STOMACH OF RATS

Abstract

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Purpose of the work was to determine the correlative dependence of the ulcer defect zone on the concentration of primary and secondary lipid peroxidation products in a blood plasma of the subclavian and portal vein. Materials and methods of the study: the runs involved 20 Wistar rats of both sexes weighed 180-230 g. The animals were kept under normal conditions of the vivarium on a standard diet. The animals were modeled with an experimental gastric ulcer acetate method S. Okabe (2005). After 7 days, animals we took the animals out of the experiment, obtained plasma from the portal and subclavian veins, visually evaluated and measured the area of the ulcer by using the planimetry. The blood plasma from the subclavian and portal veins was determined for the content of diene conjugate (primary product of lipid peroxidation) and malondialdehyde (secondary product of lipid peroxidation). For statistical processing of the results of research we used Student's t test, and Pearson’s correlation analysis. Results: the area of an experimental acetate experimental gastric ulcer after 7 days from the modeling amounted to 30.95±6.7 mm². After 7 days from the modeling of the ulcer we observed a weak correlative dependence between the square of an experimental gastric ulcers and primary content (r=0.11), (r=0.23) and secondary (r=0.26), (r=0.27) peroxidation products in a blood plasma of the subclavian and portal vein respectively. Conclusions: thus, the intensity of peroxidation in plasma obtained from the subclavian and portal vein does not significantly affect the size of gastric ulcer in rats with experimental acetate ulcer.