DAUCUS CAROTA FRUITS INFLUENCE ON CERTAIN LINKS OF ANTIOXIDANT PROTECTION OF ANIMALS WITH PARACETAMOL INTOXICATION

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Purpose of the work is the study for an influence of Daucus carota on the course of free-radical oxidation; capability to increase the resistance of cells to lipid peroxidation in the conditions of medical intoxication, provoked by paracetamol. Materials and methods of the study: to achieve a desired goal, we obtained a powder from the fruits of Daucus carota, which was injected during 14 days into stomachs of healthy animals. The powder was administered at dose 250 mg/kg as a suspension on 5% starch at volume of 2.0 ml. Control animal group received 5% solution of starch at the same volume. The second series of runs was carried out in the conditions of pharmaceutical intoxication with paracetamol. The experiment was conducted following the same scheme. We administrated paracetamol at toxic dose 3.5 mg/kg on 7th and 8th day of the experiment, an hour after the injection of the substance under study. Control group was injected with 5% solution of starch with paracetamol. Every group had 10 animals. Rats were kept in standard conditions of vivarium. Corn silks were used as a comparison drug. After the tests termination, animals were decapitated. The level of lipid peroxidation was determined in blood serum using general methods, by the content of malondialdehyde (MDA), thiobarbituric acid-active products (TBA-AP) and diene conjucates (DC). Antioxidant action was studied by the activity of catalase, superoxide dismutase (SOD) in post-nuclear fraction of liver (PFL) and the level of antioxidant activity (AOA). Results: course injection of the powder of the fruits of Daucus carota relevantly decreased the level of lipid peroxidation and activated an antioxidant system of healthy animals which follows after decrease of DC, TBA-AP, MDA content and increase of catalase activity, SOD and AOA in comparison with the intact and control groups of animals. The powder of Daucus carota decreased the content of DC by 27.2%, TBA-AP decreased by 37.0% in relation to an intact group of animals. MA level did not exceed the normal index in all groups under study. Activity of catalase increased by 22.2% in healthy animals; content of SOD in PFL increased by 18.7% after the application of the powder of Daucus carota. The substance under study influenced positively the AOA percent. The powder effect from corn silks did not differ significantly from the action of the powder of Daucus carota. After the appliance of paracetamol in the control group we encountered in a significant lipid peroxidation level elevation. The content of DC, TBA-AP, and MA increased several times in relation to the healthy animals. The work of antioxidant system was disturbed; catalase activity decreased by 28.1%, and SOD index decreased by 56.0%. Animals group with intoxication had the same indices after the injection of the substances under study, and their inclination towards the level of healthy animals indices. The powder of Daucus carota manifested a stable antioxidant effect. The catalase level increased in relation to the control group of animals by 52.5%; activity of SOD in blood serum increased by 119.8%; percent of AOA amounted to 59.8%. This effect was comparable with the action of comparison substance - corn silks, and exceeded them by several indices. Conclusions: the powder of Daucus carota conduces the reduction of lipid peroxidation level and activates antioxidant mechanisms. The results obtained provide grounds for further researches with the objective to obtain new drugs with antioxidant properties.