A marker of proliferative activity Ki-67 in assessing the regeneration of the ciliated epithelium after a nasal injury in an experiment


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Introduction. Inflammation that occurs in the nasal mucosa in response to surgical trauma is characterized by the development of alterative-dystrophic, vascular-exudative and proliferative reactions. One of the most widely used methods in practice for quantifying proliferative activity is the method of immunohistochemical detection of Ki-67 protein expression. The purpose of the study was to quantify the proliferative activity of epithelial cells after injury to the nasal cavity in an experiment under the influence of regenerative therapy. Methods. A preclinical randomized experimental study was conducted on 480 white male rats after modeling their experimental rhinitis. The animals were divided into 6 groups depending on the prescribed regenerative therapy (0.25% solution of sodium deoxyribonucleate, dexpanthenol, sodium hyaluronate). Results. The immunohistochemical determination of the proliferation marker of the non-histone protein Ki-67, expressed in all cells in the process of preparation and actually in mitosis, seems relevant and allows it to be used as a universal marker of proliferating cells. Quantitative assessment of the proliferative activity of epithelial cells in dynamics is relevant, as it allows us to assess the effectiveness and safety of regenerative therapy.

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Iryna Berest

Federal State Budgetary Educational Institution of Higher Education "Saint Luke Lugansk State Medical University" of the Ministry of health of the Russian Federation

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Email: i_berest@mail.ru
ORCID iD: 0000-0003-1694-341X
SPIN 代码: 3837-3276

Доцент кафедры оториноларингологии и офтальмологии 

俄罗斯联邦, 291045, Lugansk, Russia, 50-letiya Oborony Luganska kv., 1G

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