Innovation methods of beta vulgaris l. molecular breeding

Cover Page

Abstract


Molecular-genetic selection of sugar beet parental lines for hybridization has been performed. The results of molecular evaluation of sugar beet initial lines using microsattelite markers are presented. Object of the investigations are plants of male sterile lines and linesmultigerm pollinators. The investigations of polymorphism of 12 microsattelite markers have resulted in selection of 2 primer pairs –
Bvv 30 + Bvv 64 and Bvv 23 + Bvv 32 – as the most informative to reveal heterogeneity of starting material and effective to forecast heterosis. The PCR-analysis of 12 sugar beet initial lines with these pairs of microsattelite primers has allowed determination of specific DNA-profiles for breeding material. Genetical distances have been determined, and the cluster analysis has been performed that allow differentiation of the studied varieties between clusters depending on genetical relationship. Parental forms are recommended for crosses to produce highly productive hybrids. It has been revealed that crossbreeding of the lines being the most distantly related genetically plays an important part in exhibiting heterosis effect in hybrids. It is advisable to exclude hybrid combinations with genetical distance less than 1.41 from further studying in connection with low efficiency. The investigations results presented are important for sugar beet practical breeding when producing heterosis hybrids.


About the authors

N. N. Bogacheva

The A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar

Author for correspondence.
Email: arpnal@rambler.ru

Russian Federation, Voronezhskaya oblast, Ramonsky ryion, p. VNIISS

candidate of biological sciences

T. P. Fedulova

The A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar

Email: arpnal@rambler.ru

Russian Federation, Voronezhskaya oblast, Ramonsky ryion, p. VNIISS

doctor of biological sciences

A. A. Nalbandyan

The A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar

Email: arpnal@rambler.ru

Russian Federation, Voronezhskaya oblast, Ramonsky ryion, p. VNIISS

doctor of biological sciences

References

  1. Gärtner T.,Steinfath M., Andorf S., Lisec Jan, Meyer Rhonda C., Altmann Th., Willmitzer L., Selbig J.Improved Heterosis Prediction by Combining Information on DNAand Metabolic Markers // PLoS One.– 2009. –4 (4).
  2. Srivastava S., Pathak A., Kumar R., Joshi B. Genetic diversity of sugar beet genotypes evaluated by microsatellite DNA markers // Journal of Environmental Biology. – 2017. – V.37. – P 777-783.
  3. Iquebal M., Jaiswal S., Angadi U., Sablok G., Arora V. SBMDb: first whole genome putative microsatellite DNA marker database of sugarbeet for bioenergy and industrial application // Database. –2015. – V.111. – P.1-10.
  4. Izzatullayeva V., Akparov Z., Babayeva S., Ojaghi D. Efficiency of using RAPD and ISSR markers in evaluation genetic diversity in sugar beet // Turkish Journal of Biology. – 2014. – V.38. – P.429-438.
  5. Zakrzewski F., Wenke T., Holtgrawe D.,Weisshaar B., Schmidt Th. Analysis of a Cot-1 library enables the targeted identification of minisatellite and satellite families in Beta vulgaris // BMC Plant Biology. –2010. – V.10. – 8. – P.5-14.
  6. Хемлебен В.,Беридзе Т.Г., Бахман Л., Коварик Я., Торрес Р. Сателлитные ДНК // Успехи биологической химии. –2003. – Т.43. – С. 267-306.
  7. Sandhu S., Sarao N., Goyal M., Uppal SK. Profiling of sugar beet genotypes for agronomical, sugar quality and forage traits and their genetic diversity analysis using SSR markers // Electronic Journal of Plant Breeding. –2015. – V.7. – P. 253-266.
  8. Taski-Ajdukovic K., Nagi N., Curcic Z., Zoric M. Estimation of genetic diversity and relationship in sugar beet pollinators based on SSR markers // Electronic Journal of Biotechnology. –2017. – V.27. – P. 1-7.
  9. Abbasi Z., Arzani A., Majidi M. Evaluation of Genetic Diversity of Sugar Beet (Beta vulgaris L.) Crossing Parents Using Agro-morphological Traits and Molecular Markers // J. Agr. Sci. – 2014. – V.16. – P. 1397-1411.
  10. Stancic I., Zivic J., Petrovic S., Knezevic D. Impact of Genes and Proportional Contribution of Parental Genotypes to Inheritance of Root Yield and Sugar Content in Diploid Hybrids of Sugar Beet // The Scientific World Journal. –2014. – Article ID 580623. – P. 1-5.
  11. Chomczynski P.,Sacchi N. Single-StepMetod of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol- Chloroform Extraction // Anal. Biochem. – 1987. – V.162. – P. 156-159.
  12. Rogers, S.O., Bendich A.J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues // Plant Molecular Biologi. – 1985. – V.5. – P. 69-67.
  13. Smulders M.J.M., Esselink G.D., Everaert I., Riek J.De, Vosman B. Characterisation of sugar beet (Beta vulgaris L. ssp. vulgaris) varieties using microsatellite markers // ВМС Genetics. – 2010. – V.11(41). – P. 1-11.
  14. Омаров, Д.С. К методике учета и оценки гетерозиса у растений // С.-х. биология. – 1975. – Т.10. – № 1. – C. 123–127. 15. Хуссейн А.С., Богачева Н.Н., Налбандян А.А. Полиморфизм селекционных линий сахарной свеклы по микро- сателлитным локусам //Доклады Российской акаде- мии сельскохозяйственных наук. – 2016. – №2-3. – С. 10-13.

Statistics

Views

Abstract - 137

PDF (Russian) - 27

Cited-By


PlumX

Refbacks

  • There are currently no refbacks.

Copyright (c) 1970 Russian academy of sciences

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies