Innovation methods of beta vulgaris l. molecular breeding

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Abstract

Molecular-genetic selection of sugar beet parental lines for hybridization has been performed. The results of molecular evaluation of sugar beet initial lines using microsattelite markers are presented. Object of the investigations are plants of male sterile lines and linesmultigerm pollinators. The investigations of polymorphism of 12 microsattelite markers have resulted in selection of 2 primer pairs –
Bvv 30 + Bvv 64 and Bvv 23 + Bvv 32 – as the most informative to reveal heterogeneity of starting material and effective to forecast heterosis. The PCR-analysis of 12 sugar beet initial lines with these pairs of microsattelite primers has allowed determination of specific DNA-profiles for breeding material. Genetical distances have been determined, and the cluster analysis has been performed that allow differentiation of the studied varieties between clusters depending on genetical relationship. Parental forms are recommended for crosses to produce highly productive hybrids. It has been revealed that crossbreeding of the lines being the most distantly related genetically plays an important part in exhibiting heterosis effect in hybrids. It is advisable to exclude hybrid combinations with genetical distance less than 1.41 from further studying in connection with low efficiency. The investigations results presented are important for sugar beet practical breeding when producing heterosis hybrids.

About the authors

N. N. Bogacheva

The A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar

Author for correspondence.
Email: arpnal@rambler.ru

candidate of biological sciences

Russian Federation, Voronezhskaya oblast, Ramonsky ryion, p. VNIISS

T. P. Fedulova

The A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar

Email: arpnal@rambler.ru

doctor of biological sciences

Russian Federation, Voronezhskaya oblast, Ramonsky ryion, p. VNIISS

A. A. Nalbandyan

The A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar

Email: arpnal@rambler.ru

doctor of biological sciences

Russian Federation, Voronezhskaya oblast, Ramonsky ryion, p. VNIISS

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