The severity of COVID-19 is accompanied by a change in the expression of the surface marker CD38 in T lymphocytes and NK cells

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Abstract

BACKGROUND: COVID-19 mediates activation of immunocompetent cells. Little is known about the phenotypic marker CD38 on the surface of T and NK cells and its association with the severity of COVID-19.

AIM: To analyze the distribution of the surface marker CD38 in subsets of T and NK cells at the different stages of their differentiation in the groups of patients with COVID-19 and convalescents, and to reveal the relationship between the level of CD38 expression in subpopulations of T and NK cells with the severity of COVID-19.

MATERIALS AND METHODS: Peripheral blood mononuclear cells (PBMC) from patients and donors were isolated using ficoll density gradient followed by cytofluorimetric analysis of the surface markers: CD3, CD56, CD38.

RESULTS: In PBMC samples the level of CD38 expression on NK cells (CD3–CD56+), including CD56bright and CD56dim subsets, as well as on T lymphocytes, differentiating conventional CD56–(CD3+CD56–) and NKT-like (CD3+CD56+) cells, were analyzed. Among CD56– T cells and NKT-like cells the highest levels of CD38 expression were registered in the groups of patients of moderate severity and convalescents, and, in contrast, the decreased CD38 levels were detected in the group of patients from the intensive care unit.

CONCLUSIONS: The findings suggest that the level of CD38+ T cell in COVID-19 may be of use as diagnostic value.

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About the authors

Julia D. Vavilova

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Author for correspondence.
Email: juliateterina12@gmail.com
ORCID iD: 0000-0002-9075-218X

Junior Research Associate

Russian Federation, Moscow

Anna A. Boyko

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: boyko_anna@mail.ru
ORCID iD: 0000-0002-8996-2905

Cand. Sci. (Biol.), Research Associate

Russian Federation, Moscow

Maria A. Streltsova

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: mstreltsova@mail.ru
ORCID iD: 0000-0002-5403-0753
Scopus Author ID: 57077066400

Cand. Sci. (Biol.), Research Associate

Russian Federation, Moscow

Sofya A. Kust

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: sonya.erokhina@gmail.com
ORCID iD: 0000-0001-6365-0279

Cand. Sci. (Biol.), Junior Research Associate

Russian Federation, Moscow

Gaukhar M. Yusubalieva

Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies FMBA of Russia

Email: gaukhar@gaukhar.org
ORCID iD: 0000-0003-3056-4889
Scopus Author ID: 16041151500

MD, Cand. Sci. (Med.), Senior Research Associate

Russian Federation, Moscow

Oksana N. Novikova

Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies FMBA of Russia

Email: Novikova_oksana_@mail.ru
ORCID iD: 0000-0003-2779-0383

MD, Cand. Sci. (Med.), Head of the Therapeutic Department

Russian Federation, Moscow

Anna G. Sotnikova

Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies FMBA of Russia

Email: sotnikoffaa@gmail.com

MD, Cand. Sci. (Med.), Head of the Department of Pulmonology

Russian Federation, Moscow

Mikhail V. Bychinin

Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies FMBA of Russia

Email: drbychinin@gmail.com
ORCID iD: 0000-0001-8461-4867
Scopus Author ID: 57221999178

MD, Cand. Sci. (Med.), Head of the Department of Resuscitation and Intensive Care

Russian Federation, Moscow

Rustam N. Ischakov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: Rustam110798Rus@gmail.com

Student

Russian Federation, Moscow

Alexander M. Sapozhnikov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: amsap@mail.ru
ORCID iD: 0000-0002-4934-677X
Scopus Author ID: 7006548229

Dr. Sci. (Biol.), Professor, Head of the Laboratory of Cellular Interactions

Moscow

Elena I. Kovalenko

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: lenkovalen@mail.ru
ORCID iD: 0000-0001-8119-8247
Scopus Author ID: 7102778244
ResearcherId: S-2086-2016

Cand. Sci. (Biol.), Senior Research Associate

Russian Federation, Moscow

References

  1. Abdullaev A, Odilov A, Ershler M, et al. Viral load and patterns of SARS-CoV-2 dissemination to the lungs, mediastinal lymph nodes, and spleen of patients with COVID-19 associated lymphopenia. Viruses. 2021;13(7):1410. doi: 10.3390/V13071410
  2. Godfrey DI, MacDonald HR, Kronenberg M, et al. NKT cells: what’s in a name? Nat Rev. Immunol. 2004;4(3):231–237. doi: 10.1038/NRI1309
  3. Vivier E, Tomasello E, Baratin M, et al. Functions of natural killer cells. Nat Immunol. 2008;9(5):503–510. doi: 10.1038/NI1582
  4. Du J, Wei L, Li G, et al. Persistent high percentage of HLA-DR+CD38high CD8+ T cells associated with immune disorder and disease severity of COVID-19. Front Immunol. 2021;12:735125. doi: 10.3389/fimmu.2021.735125
  5. Zambello R, Barilà G, Manni S, et al. NK cells and CD38: Implication for (immuno)therapy in plasma cell dyscrasias. Cells. 2020;9(3):768. doi: 10.3390/CELLS9030768
  6. Magnone M, Bauer I, Poggi A, et al. NAD+ levels control Ca2+ store replenishment and Mitogen-induced Increase of Cytosolic Ca2+ by Cyclic ADP-ribose-dependent TRPM2 channel gating in human T lymphocytes. J Biol Chem. 2012;287(25):21067–21081. doi: 10.1074/JBC.M111.324269
  7. Maucourant C, Filipovic I, Ponzetta A, et al. Natural killer cell immunotypes related to COVID-19 disease severity. Sci Immunol. 2020;5(50):eabd6832. doi: 10.1126/SCIIMMUNOL.ABD6832
  8. Chini CCS, Peclat TR, Warner GM, et al. CD38 ecto-enzyme in immune cells is induced during aging and regulates NAD+ and NMN levels. Nat Metab. 2020;2(11):1284–1304. doi: 10.1038/s42255-020-00298-z
  9. Schiavoni I, Scagnolari C, Horenstein AL, et al. CD38 modulates respiratory syncytial virus-driven proinflammatory processes in human monocyte-derived dendritic cells. Immunology. 2018;154(1):122–131. doi: 10.1111/IMM.12873
  10. Zeidler JD, Kashyap S, Hogan KA, Chini EN. Implications of the nadase CD38 in COVID pathophysiology. Physiol Rev. 2022;102:339–341. doi: 10.1152/PHYSREV.00007.2021
  11. Dai M, Liu D, Liu M, et al. Patients with cancer appear more vulnerable to SARS-CoV-2: A multicenter study during the COVID-19 outbreak. Cancer Discov. 2020;10(6):783–791. doi: 10.1158/2159-8290.CD-20-0422
  12. Van Eeden C, Khan L, Osman MS, Tervaert JWC. Natural killer cell dysfunction and its role in COVID-19. Int J Mol Sci. 2020;21(17):6351. doi: 10.3390/IJMS21176351
  13. Menzies FM, Fleming A, Caricasole A, et al. Autophagy and neurodegeneration: pathogenic mechanisms and therapeutic opportunities. Neuron. 2017;93:(5):1015–1034. doi: 10.1016/j.neuron.2017.01.022
  14. Morandi F, Horenstein AL, Chillemi A, et al. CD56brightCD16− NK cells produce adenosine through a CD38-mediated pathway and act as regulatory cells inhibiting autologous CD4+ T Cell proliferation. J Immunol. 2015;195(3):965–972. doi: 10.4049/jimmunol.1500591
  15. Horenstein AL, Faini AC, Malavasi F. CD38 in the age of COVID-19: Amedical perspective. Physiol Rev. 2021;101(4):1457–1486. doi: 10.1152/physrev.00046.2020

Supplementary files

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2. Fig. 1. Gating strategy for cell subpopulations in flow cytometry data analysis

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3. Fig. 2. Proportion of CD38+ cells in the populations of CD56– T cells (a) and NKT-like cells (b) in the group of the intensive care unit (ICU) (n = 19), moderate patients (n = 24), convalescents (n = 29) and healthy volunteers (n = 28). n is the number of volunteers in the group. * p < 0.05, ** p < 0.01, *** p < 0.001

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4. Fig. 3. Proportion of CD38+ cells in populations of NK cells (a), CD56bright NK cells (b) and CD56dim NK cells (c) in the group of: intensive care unit (ICU) patients (n = 19), moderate patients (n = 28), convalescents (n = 33) and healthy volunteers (n = 28). n is the number of volunteers in the group. * p < 0.05, ** p < 0.01, *** p < 0.001

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