Sex features of the circadian rhythmicity of some biochemical parameters in wistar rats under constant lighting and chronic alcohol intoxication
- Authors: Areshidze D.A.1, Kozlova M.A.1, Makartseva L.A.1, Kirillov Y.A.1
-
Affiliations:
- A.P. Avtsyn Research Institute of Human Morphology, Petrovsky National Research Centre of Surgery
- Issue: Vol 22, No 3 (2022)
- Pages: 49-60
- Section: Original research
- URL: https://journals.eco-vector.com/MAJ/article/view/108606
- DOI: https://doi.org/10.17816/MAJ108606
- ID: 108606
Cite item
Abstract
BACKGROUND: Biological systems of all levels of organization are characterized by the rhythm of functioning processes, which are one of the fundamental properties of living matter. The complex of circadian rhythms of mammals, being genetically determined, is quite plastically modulated by the action of periodic factors of the external and internal environment. Significant factors in the disorganization of biorhythms in the modern world include a violation of the light-dark regime, so-called light pollution. Alcohol abuse remains one of the most important medical and social problems of modern society. One of the important effects of alcohol is its destructive effect on the circadian rhythms of many physiological processes.
AIM: The aim of the research was to study the influence of constant lighting, chronic alcohol intoxication and joint effect of these factors on the diurnal dynamics of several biochemical parameters in Wistar rats of both sexes.
MATERIALS AND METHODS: The study was conducted on 200 and 160 female outbred Wistar rats at the age of 6 months, weighing 350 ± 15 g. Rats were divided into 8 groups. 1st group (control ♂) is kept at fixed light regime (light/dark 10:14 hours with lights on at 8:00 and off at 18:00). 2nd group, males (n = 50) is kept under the same conditions as the control, but receives a 15 % aqueous solution of ethanol ad libitum as a drink instead of water, i.e. is subjected to chronic alcohol intoxication. Group 3, males (n = 50) are kept under constant light. The 4th group, males (n = 50) are also kept under constant illumination and receive 15 % aqueous ethanol solution ad libitum as a drink. Group 5 (control ♀) females (n = 40), are kept at a fixed light regime (light/dark 10:14 am with lights on at 8:00 and off at 18:00). The 6th group, females (n = 40) are kept under the same conditions as the control, but receive 15 % aqueous ethanol solution ad libitum instead of water as a drink, i.e. subjected to chronic alcohol intoxication. Group 7, females (n = 40) are kept under constant light. The 8th group, females (n = 40) are also kept under constant light and receive 15 % aqueous ethanol solution ad libitum as a drink. In the blood samples taken at 9:00, 15:00, 21:00 and 3:00 hours the content of AST, ALT, glucose, total protein, albumin, total and direct bilirubin was measured. The reliability of circadian rhythmicity of studied parameters was assessed with use of cosinor analysis.
RESULTS: It is established that the chronic alcohol intoxication, constant illumination and joint action of this factors lead to similar changes in biochemical parameters in rats of both sexes, but in female rats the level of AST, total and direct bilirubin changes as a result of three weeks of intoxication, which is not observed in males. In turn, both individual and joint effects of chronic alcohol intoxication and dark deprivation lead to significant changes in rhythmostasis in rats, however, circadian rhythms of total protein, as well as both types of bilirubin, are more resistant to dark deprivation in females than in males.
CONCLUSIONS: The conducted study testifies that a three-week chronic alcohol intoxication causes more significant changes in the biochemical profile in female rats compared to males. At the same time, the studied circadian rhythms of the biochemical parameters of the organism of females turn out to be more resistant to dark deprivation than those of males, being destroyed only under the combined action of chronic alcohol intoxication and constant illumination.
Full Text
About the authors
David A. Areshidze
A.P. Avtsyn Research Institute of Human Morphology, Petrovsky National Research Centre of Surgery
Author for correspondence.
Email: labcelpat@mail.ru
ORCID iD: 0000-0003-3006-6281
SPIN-code: 4348-6781
Scopus Author ID: 55929152900
ResearcherId: G-8387-2014
Cand. Sci. (Biol.), Leading Research Associate, Head of the Laboratory of Pathology of Cell
Russian Federation, MoscowMaria A. Kozlova
Email: ma.kozlova2021@outlook.com
ORCID iD: 0000-0001-6251-2560
SPIN-code: 5647-1372
Scopus Author ID: 55976515700
ResearcherId: AAE-5096-2021
Research Associate of the Laboratory of Pathology of Cell
Russian FederationLyudmila A. Makartseva
A.P. Avtsyn Research Institute of Human Morphology, Petrovsky National Research Centre of Surgery
Email: la.makartseva@outlook.com
ORCID iD: 0000-0002-1882-8848
SPIN-code: 4254-1571
Scopus Author ID: 57201418859
ResearcherId: AAE-5136-2021
Junior Research Associate of the Laboratory of Pathology of Cell
Russian Federation, MoscowYuri A. Kirillov
A.P. Avtsyn Research Institute of Human Morphology, Petrovsky National Research Centre of Surgery
Email: nihilist78@mail.ru
ORCID iD: 0000-0003-3555-0902
SPIN-code: 6514-5577
Scopus Author ID: 56531783200
ResearcherId: AAE-7630-2021
MD, Dr. Sci. (Med.), Leading Research Associate of the Laboratory of Clinical Morphology
Russian Federation, MoscowReferences
- McKenna H, van der Horst GTJ, Reiss I, Martin D. Clinical chronobiology: a timely consideration in critical care medicine. Crit Care. 2018;22(1):124. doi: 10.1186/s13054-018-2041-x
- Michel S, Meijer JH. From clock to functional pacemaker. Eur J Neurosci. 2020;51(1):482–493. doi: 10.1111/ejn.14388
- Panda S. Circadian physiology of metabolism. Science. 2016;354(6315):1008–1015. doi: 10.1126/science.aah4967
- Zimmet P, Alberti KGMM, Stern N, et al. The circadian syndrome: is the metabolic syndrome and much more! J Intern Med. 2019;286(2):181–191. doi: 10.1111/joim.12924
- Verlande A, Masri S. Circadian clocks and cancer: Timekeeping governs cellular metabolism. Trends Endocrinol Metab. 2019;30(7):445–458. doi: 10.1016/j.tem.2019.05.001
- Alyakrinskii BS. Biologicheskie ritmy i organizatsiya zhizni cheloveka v kosmose. Vol. 46. Series “Problemy kosmicheskoi biologii”. Moscow: Nauka; 1983. 248 p. (In Russ.)
- Zucker I, Beery AK. Males still dominate animal studies. Nature. 2010;465(7299):690. doi: 10.1038/465690a
- Kuljis DA, Loh DH, Truong D, et al. Gonadal- and sex-chromosome-dependent sex differences in the circadian system. Endocrinology. 2013;154(4):1501–1512. doi: 10.1210/en.2012-1921
- Estevez ME, Fogerson PM, Ilardi MC, et al. Form and function of the M4 cell, an intrinsically photosensitive retinal ganglion cell type contributing to geniculocortical vision. J Neurosci. 2012;32(39):13608–13620. doi: 10.1523/JNEUROSCI.1422-12.2012
- Bailey M, Silver R. Sex differences in circadian timing systems: implications for disease. Front Neuroendocrinol. 2014;35(1):111–139. doi: 10.1016/j.yfrne.2013.11.003
- Zhang YK, Yeager RL, Klaassen CD. Circadian expression profiles of drug-processing genes and transcription factors in mouse liver. Drug Metab Dispos. 2009;37(1):106–115. doi: 10.1124/dmd.108.024174
- Justo R, Boada J, Frontera M, et al. Gender dimorphism in rat liver mitochondrial oxidative metabolism and biogenesis. Am J Physiol Cell Physiol. 2005;289(2):C372–378. doi: 10.1152/ajpcell.00035.2005
- Zheng D, Wang X, Antonson P, et al. Genomics of sex hormone receptor signaling in hepatic sexual dimorphism. Mol Cell Endocrinol. 2018;471:33–41. doi: 10.1016/j.mce.2017.05.025
- Hirao J, Nishimura M, Arakawa S, et al. Sex and circadian modulatory effects on rat liver as assessed by transcriptome analyses. J Toxicol Sci. 2011;36(1):9–22. doi: 10.2131/jts.36
- Xu YQ, Zhang D, Jin T, et al. Diurnal variation of hepatic antioxidant gene expression in mice. PLoS One. 2012;7(8):e44237. doi: 10.1371/journal.pone.0044237
- Fárková E, Schneider J, Šmotek M, et al. Weight loss in conservative treatment of obesity in women is associated with physical activity and circadian phenotype: a longitudinal observational study. Biopsychosoc Med. 2019;13:24. doi: 10.1186/s13030-019-0163-2
- Poggiogalle E, Jamshed H, Peterson CM. Circadian regulation of glucose, lipid, and energy metabolism in humans. Metabolism. 2018;84:11–27. doi: 10.1016/j.metabol.2017.11.017
- Yalçin M, El-Athman R, Ouk K, et al. Analysis of the circadian regulation of cancer hallmarks by a cross-platform study of colorectal cancer time-series data reveals an association with genes involved in Huntington’s disease. Cancers (Basel). 2020;12(4):963. doi: 10.3390/cancers12040963
- Bailey SM. Emerging role of circadian clock disruption in alcohol-induced liver disease. Am J Physiol Gastrointest Liver Physiol. 2018;315(3):G364–G373. doi: 10.1152/ajpgi.00010.2018
- Kozlova MA, Kirillov YA, Makartseva LA, et al. Morphofunctional state and circadian rhythms of the liver under the influence of chronic alcohol intoxication and constant lighting. Int J Mol Sci. 2021;22(23):13007. doi: 10.3390/ijms222313007
- Cornelissen G. Cosinor-based rhythmometry. Theor Biol Med Model. 2014;11:1–24. doi: 10.1186/1742-4682-11-16
- Idrovo JP, Shults JA, Curtis BJ, et al. Alcohol intoxication and the postburn gastrointestinal hormonal response. J Burn Care Res. 2019;40(6):785–791. doi: 10.1093/jbcr/irz083
- Lin Y, Ying YY, Li SX, et al. Association between alcohol consumption and metabolic syndrome among Chinese adults. Public Health Nutr. 2021;24(14):4582–4590. doi: 10.1017/S1368980020004449
- Abdullaev SM, Mukhin NA. Spravochnik po gepatologii. Moscow: Litterra; 2009. (In Russ.)
- Roslyi IM, Vodolazhskaya MG. Pravila chteniya biokhimicheskogo analiza. Rukovodstvo dlya vracha. 3rd ed. Moscow; 2020. (In Russ.)
- Oishi K, Amagai N, Shirai H, et al. Genome-wide expression analysis reveals 100 adrenal gland-dependent circadian genes in the mouse liver. DNA Res. 2005;12(3):191–202. doi: 10.1093/dnares/dsi003
- Chrousos GP, Kino T. Intracellular glucocorticoid signaling: a formerly simple system turns stochastic. Sci STKE. 2005;2005(304):pe48. doi: 10.1126/stke.3042005pe48
- Kloehn I, Pillai SB, Officer L, et al. Sexual differentiation of circadian clock function in the adrenal gland. Endocrinology. 2016;157(5):1895–1904. doi: 10.1210/en.2015-1968
- Cain SW, Dennison CF, Zeitzer JM, et al. Sex differences in phase angle of entrainment and melatonin amplitude in humans. J Biol Rhythms. 2010;25(4):288–296. doi: 10.1177/0748730410374943
- Dallmann R, Touma C, Palme R, et al. Impaired daily glucocorticoid rhythm in Per1 (Brd) mice. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2006;192(7):769–775. doi: 10.1007/s00359-006-0114-9