CIRCULATING NEUTROPHIL EXTRACELLULAR TRAPS IN TYPE 2 DIABETES MELLITUS PATIENTS WITH SUPPURATIVE AND NECROTIC COMPLICATIONS



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Aim of the study. Neutrophil extracellular traps (NETs) have been reported to participate in many pathological processes, which points at NET concentration in blood as an important indicator of the body condition. When studying NETs, their blood concentration is usually evaluated indirectly, which has a number of shortcomings. We aimed to measure directly the amount of NETs as integral structures, using blood smears as a most suitable tool for clinical practice, and to carry out measurements of NET levels in blood of type 2 diabetes mellitus (T2DM) patients with necrotic suppurative complications to elucidate the usefulness of this assay. Materials and methods of the research. NETs were quantified by counting in standardized blood smears stained with Romanovsky dyes. Results. The mean level of circulating NETs in the blood of patients was higher than that in healthy subjects. Blood NET concentration in healthy persons and patients with high levels of NETs increased as glycated hemoglobin (HbA1c) concentration increased in the blood. In other patients, NET concentration decreased as HbAlc increased. Elevated NET levels and a direct correlation between NETs and HbAlc were observed in patients with phlegmon, large-area infected wound, lower leg gangrene, suppurative mediastinitis. In other patients, the prevailing lesions were infected wounds, abscesses, finger gangrene. Conclusion. This pilot study demonstrated the usefulness of measurement (using blood smear) of circulating NET concentration in the blood as a clinical parameter that would help to estimate patient’s condition, to predict the duration of post-surgical wound healing and the probability of complications in the form of thrombosis in T2DM.

Full Text

Restricted Access

About the authors

D I Maximov

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

L Yu Basyreva

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

A A Gusev

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

T V Vakhrusheva

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

A V Yaskevich

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

S A Gusev

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

E M Ostrovsky

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

O M Panasenko

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

V I Sergienko

FSBI «Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency»

References

  1. Brinkmann V., Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss D.S., Weinrauch Y., Zychlinsky A. Neutrophil extracellular traps kill bacteria // Science. 2004. Vol. 303, No 5663. P. 1532-1535.
  2. Brinkmann V, Zychlinsky A. Neutrophil extracellular traps: is immunity the second function of chromatin // J. Cell Biol. 2012. Vol. 198, No 5. P. 773-783.
  3. Marin-Esteban V., Turbica I., Dufour G., Semiramoth N, Gleizes A, Gorges R. Afa/Dr diffusely adhering Escherichia coli strain C1845 induces neutrophil extracellular traps that kill bacteria and damage human enterocyte-like cells // Infect. Immun. 2012. Vol. 80, No 5. P. 1891-1899.
  4. Delgado-Rizo V., Martnnez-Guzman M.A., Idguez-Gutierrez L, Garcna-Orozco A., Alvarado-Navarro A., Fafutis-Morris M. Neutrophil extracellular traps and its implications in inflammation: an overview // Front. Immunol. 2017. Vol. 8. P. 81.
  5. Simon D., Simon H.U., Yousefi S. Extracellular DNA traps in allergic, infectious, and autoimmune diseases // Allergy. 2013. Vol. 68, No 4. P. 409-416.
  6. Perez-Sanchez C, Ruiz-Limyn P, Aguirre M.A., Jimenez-Gymez Y, Arias-de la Rosa I., Abalos-Aguilera M.C., Rodriguez-Ariza A, Castro-Villegas M.C., Ortega-Castro R., Segui P., Martinez C., Gonzalez-Conejero R., Rodrnguez-Lypez S., Gonzalez-Reyes J.A., Villalba J.M., Collantes-Estevez E., Escudero A., Barbarroja N., Lypez-Pedrera C. Diagnostic potential of NETosis-derived products for disease activity, atherosclerosis and therapeutic effectiveness in Rheumatoid Arthritis patients // J. Autoimmun. 2017. Vol. 82. P. 31-40.
  7. Silk E., Zhao H., Weng H., Ma D. The role of extracellular histone in organ injury // Cell Death Dis. 2017. Vol. 8. Р. 2812.
  8. Fuchs T.A., Brill A, Duerschmied D., Schaizberg D., Monestier M., Myers D.D.Jr., Wrobleski S.K., Wakefield T.W., Hartwig J.H., Wagner D.D. Extracellular DNA traps promote thrombosis // Proc. Natl. Acad. Sci. U.S.A. 2010. Vol. 107, No 36. P. 15880-15885.
  9. Lizcano A., Secundino I., Dyhrmann S., Corriden R., Rohena C., Diaz S., Ghosh P., Deng L., Nizet V., Varki A. Erythrocyte sialoglycoproteins engage Siglec-9 on neutrophils to suppress activation // Blood. 2017. Vol. 129, No 23. P. 3100-3110.
  10. Hamaguchi S., Hirose T., Akeda Y, Matsumoto N., Irisawa T, Seki M, Hosotsubo H., Tasaki O., Oishi K, Shimazu T, Tomono K. Identification of neutrophil extracellular traps in the blood of patients with systemic inflammatory response syndrome // J. Int. Med. Res. 2013. Vol. 41, No 1. P. 162-168.
  11. Beiter T, Fragasso A, Hudemann J., Schild M, Steinacker J., Mooren F.C., Niess A.M. Neutrophils release extracellular DNA traps in response to exercise // J. Appl. Physiol. 2014. Vol. 117, No 3. P. 325-333.
  12. Hirose T, Hamaguchi S, Matsumoto N, Irisawa T, Seki M, Tasaki O, Hosotsubo H, Yamamoto N, Yamamoto K, Akeda Y, Oishi K, Tomono K, Shimazu T. Presence of Neutrophil Extracellular Traps and Citrullinated Histone H3 in the Bloodstream of Critically ill Patients // PLoS One. 2014. Vol. 9, No 11. e111755.
  13. Долгушин И.И., Андреева Ю.С., Савочкина А.Ю. Нейтрофильные внеклеточные ловушки и методы оценки функционального статуса нейтрофилов. М.: Изд-во РАМН, 2009. 203 с. [Dolgushin I.I., Andreeva Yu.S., Savochkina A.Yu. Neutrophil extracellular traps and methods to assess the functional state of neutrophils. Moscow: Izdatel’stvo RAMS, 2009. 203 p.]
  14. Basyreva L.Y, Brodsky I.B., Gusev A.A., Zhapparova O.N., Mikhalchik E.V, Gusev S.A., Shor D.B., Dahan S, Blank M, Shoenfeld Y. The effect of intravenous immunoglobulin (IVIG) on ex vivo activation of human leukocytes // Hum. Antibodies. 2016. Vol. 24, No 3-4. P. 39-44.
  15. Wellen K.E., Hotamisligil G.S. Inflammation, stress, and diabetes // J. Clin. Invest. 2005. Vol. 115, No 5. P. 1115-1119.
  16. Fernandez-Real J.M., Pickup J.C. Innate immunity, insulin resistance and type 2 diabetes // Diabetologia. 2012. Vol. 55, No 2. P. 273-278.
  17. Villanueva E, Yalavarthi S, Berthier C.C., Hodgin J.B., Khandpur R, Lin A.M., Rubin C.J., Zhao W., Olsen S.H., Klinker M, Shealy D, Denny M.F., Plumas J., Chaperot L, Kretzler M, Bruce A.T., Kaplan M.J. Netting neutrophils induce endothelial damage, infiltrate tissues, and expose immunostimulatory molecules in systemic lupus erythematosus // J. Immunol. 2011. Vol. 187, No 1. P. 538-552.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2018 Maximov D.I., Basyreva L.Y., Gusev A.A., Vakhrusheva T.V., Yaskevich A.V., Gusev S.A., Ostrovsky E.M., Panasenko O.M., Sergienko V.I.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 74760 от 29.12.2018 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies